ALEXIS Biochemicals: Fas (human):Fc (human) (recombinant)

ALX-522-002

Revised 06-Jun-07

Fas (human):Fc (human) (recombinant)
SYNONYMS	APO-1 (human):Fc (human) (recombinant) CD95 (human):Fc (human) (recombinant) TNFRSF 6 (human):Fc (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Fas

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-002-C050		50 µg	360.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of human Fas (CD95; APO-1) (aa 7-154) is fused to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions can be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds human, mouse and rat FasL (APO-1L; CD95L; CD178).
BIOLOGICAL ACTIVITY:	Inhibits the activity of human and mouse soluble Fas (sFasL) and thereby sFasL-mediated lysis of Fas sensitive cells in a concentration range of 20-100µg/ml. When used with cross-linking enhancer (see Set Prod. No. ALX-850-016), the inhibitory activity of rhFas:Fc is increased approximately 20- to 50-fold. Concentrations of rhFas:Fc required to inhibit may vary depending on the cell viability and on the concentrations of sFasL used to kill the cells.
APPLICATION:	ELISA (capture, 1µg/ml).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Images

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Product Specific Literature References

Characterization of Fas (Apo-1, CD95)-Fas ligand interaction: P. Schneider, et al.; J. Biol. Chem. 272, 18827 (1997) Abstract; Full Text
Cysteine 230 is essential for the structure and activity of the cytotoxic ligand TRAIL: J.L. Bodmer, et al.; J. Biol. Chem. 275, 20632 (2000) Abstract; Full Text
Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000) Abstract
TGF-beta induces the expression of the FLICE-inhibitory protein and inhibits Fas-mediated apoptosis of microglia: R. Schlapbach, et al.; Eur. J. Immunol. 30, 3680 (2000) Abstract
Mechanisms underlying neuronal death induced by chromogranin A-activated microglia: J. Ciesielski-Treska, et al.; J. Biol. Chem. 276, 13113 (2001) Abstract; Full Text
T cell costimulation by the TNF ligand BAFF: B. Huard et al.; J. Immunol. 167, 6225 (2001) Abstract
Productive HIV-1 infection of primary CD4+ T cells induces mitochondrial membrane permeabilization leading to a caspase-independent cell death: F. Petit et al; J. Biol. Chem. 277, 1477 (2002) Abstract; Full Text
Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex: N. Holler, et al.; Mol. Cell. Biol. 23, 1428 (2003) Abstract; Full Text
High expression of Fas ligand by synovial fluid-derived gamma delta T cells in Lyme arthritis: K. Roessner, et al.; J. Immunol. 170, 2702 (2003) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P25445: Fas (human) (precursor) AfCS Signalling Gateway link A000600: Fas (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.