ALEXIS Biochemicals: Guanylyl Cyclase (α1β1), Soluble (human) (recombinant) (His) (purified)

ALX-201-177

Revised 04-Aug-08

Guanylyl Cyclase (α1β1), Soluble (human) (recombinant) (His) (purified)
SYNONYMS	GTP Pyrophosphate-lyase (cycling), Soluble (human) (recombinant) (His) (purified) sGCα₁/sGCβ₁ (human) (recombinant) (His) (purified)
PRODUCT LINE	Nitric Oxide Pathway
PRODUCT CATEGORY	Guanylyl Cyclases, Guanosine Phosphate Metabolism/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-177-C010		10 µg	295.00 USD

Product Specification

MW:	~70kDa/~86kDa / subunit, respectively; heterodimer.
EC:	4.6.1.2
SOURCE/HOST:	Produced in Sf9 cells. Fused at the C-terminus to a His-tag.
CONCENTRATION:
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. In 50mM TEA/MES, pH 7.4 containing 1mM EDTA/EGTA, 5mM GSH and 30% glycerol.
SPECIFIC ACTIVITY:	- Basal: ≥0.03µmol/mg/min. - 100µM DEA NONOate (Prod. No. ALX-430-034) ≥10µmol/mg/min. - 1µM BAY 41-2272 (Prod. No. ALX-420-030): ≥0.8µmol/mg/min.
APPLICATION:	Biophysical and enzymatic studies and drug screening.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-80°C
USE/STABILITY:	We do not recommend to store for longer periods after opening and thawing of the product.
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Soluble guanylate cyclase purified from bovine lung contains heme and copper: R. Gerzer, et al.; FEBS Lett. 132, 71 (1981) Abstract
Human soluble guanylate cyclase: functional expression and revised isoenzyme family: U. Zabel, et al.; Biochem. J. 335 (Pt 1), 51 (1998) Abstract; Full Text
Purified soluble guanylyl cyclase expressed in a baculovirus/Sf9 system: stimulation by YC-1, nitric oxide, and carbon monoxide: M. Hoenicka, et al.; J. Mol. Med. 77, 14 (1999) Abstract
Human recombinant soluble guanylyl cyclase: expression, purification, and regulation: Y.C. Lee, et al.; PNAS 97, 10763 (2000) Abstract; Full Text
Human soluble guanylate cyclase: functional expression, purification and structural characterization: D.N. Kosarikov, et al.; Arch. Biochem. Biophys. 388, 185 (2001) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-prot link:
Human α₁ subunit-accession AAB94794, locus CYG3_human
Human β₁ subunit-accession AAH47620, locus CYG1_human

Further Categories Containing This Product:

Enzymes • Recombinant Proteins/Fusion Proteins

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