ALEXIS Biochemicals: HAT Activity Assay Kit

ALX-850-326

Revised 07-Jan-08

HAT Activity Assay Kit
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Histone Acetyl- & Methyl-Transferases/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-326-KI01		1 Kit	413.00 USD

Product Specification

QUANTITY:	~100 tests.
KIT/SET CONTAINS:	2x HAT Assay Buffer HAT Substrate I HAT Substrate II NADH Generating Enzyme NE (Nuclear Extract 4 mg/ml) HAT Reconstitution Buffer
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C

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Product Description

Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active recombinant HAT as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.

Product Specific Literature References

Quercetin inhibits TNF-induced NF-kappaB transcription factor recruitment to proinflammatory gene promoters in murine intestinal epithelial cells: P.A. Ruiz, et al.; J. Nutr. 137, 1208 (2007) Abstract

General Information

MANUFACTURER

Manufactured by BioVision.

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