ALEXIS Biochemicals: HNE-histidine STRESS ELISA Kit

ALX-850-322

Revised 03-Jul-08

HNE-histidine STRESS ELISA Kit
PRODUCT LINE	Oxidative Stress
PRODUCT CATEGORY	Oxidative Stress Markers & Reagents/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-322-KI01		1 Kit	750.00 USD

Product Specification

SENSITIVITY:	8.1nmol/mg (0.143-1.714nmol/mg)
QUANTITY:	2x96 wells
APPLICATION:	For the quantitative determination of HNE-histidine conjugates in human cell lysates induced by strong oxidative stress.
SHIPPING:	SHIPPED ON BLUE ICE
HANDLING:	Store standards at -80°C. Store rest of the kit at +4°C.

Product Description

- Quantitative determination of HNE-histidine in human cell lysates
- Indirect enzyme immunoassay
- Based on a well described monoclonal antibody used in ICC, IHC & WB
- Range of detection between 71pmol/mg and 857pmol/mg
- Human plasma spiking tested
- Tissue homogenates have not been tested yet

(E)-4-Hydroxynonenal (HNE) is one of the most important breakdown products and reliable marker of lipid peroxidation (LPO). This indirect enzyme immunoassay (EIA) allows for the first time the quantitative determination of HNE-histidine conjugates from lysates of human cells which were exposed to strong oxidative stress.

Product Specific Literature References

Enzyme-linked immunosorbent assay for 4-hydroxynonenal-histidine conjugates: S. Borovic, et al.; Free Radic. Res. 40, 809 (2006) Abstract
Differential sensitivity to 4-hydroxynonenal for normal and malignant mesenchymal cells: S. Borovic, et al.; Redox Rep. 12, 50 (2007) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Do not use serum free cell culture supernatants. Running duplicate wells for samples and standards is recommended.

ALX-850-320	HNE-histidine FINE ELISA Kit
ALX-840-041	HNE-histidine FINE Standard
ALX-840-042	HNE-histidine STRESS Standard