ALEXIS Biochemicals: IL-33, Soluble (human) Detection Set [For ELISA Application]

APO-54N-025

Revised 08-Apr-08

IL-33, Soluble (human) Detection Set [For ELISA Application]
SYNONYMS	Interleukin-33, Soluble (human) Detection Set [For ELISA Application] NF-HEV, Soluble (human) Detection Set [For ELISA Application]
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	Interleukin Kits & Sets

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-025-KI01		1 Set	630.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SENSITIVITY:	5pg/ml (range 0 to 500pg/ml).
QUANTITY:	For 5x96 wells.
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).
APPLICATION:	For the quantitative determination of soluble human IL-33 from biological fluids (serum and cell culture supernatant). Does not cross-react with mouse IL-33.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Images

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General Information

Interleukin-1 (IL-1) family members, such as IL-1α/β and IL-18 play important roles in host defense, immune regulation, neuronal injury and inflammation [1, 2]. Recently, a new member of this family, IL-33, also named Nuclear Factor from High Endothelial Venules (NF-HEV) [3], has been reported to be the specific ligand for ST2 [4], an orphan receptor of the IL-1 receptor family [5]. Structurally, IL-33 is closest to IL-18, rather than IL-1β. The human IL-33 is expressed with a prodomain (aa 1-111) that is cleaved by caspase-1 to produce a 18kDa mature protein (112-270) [6]. The stimulation of ST2 by IL-33 induces the NF-κB and MAPK athways. IL-33 is synthesized in response to cytokines such as IL-1β (but also TNFα) and plays an important role in T_H2-associated immunology, by triggering an increase secretion of IL-5 and IL-13 from polarized T_H2 cells. Overexpression of IL-33 in mice leads to severe pathological changes in mucosal organs.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q2YEJ5: Interleukin-33 (human)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

[1] Differences in signaling pathways by IL-1beta and IL-18: J.K. Lee, et al. ; Proc. Natl. Acad. Sci. 101, 8815 (2004) Abstract
[2] Interleukin-1 and neuronal injury: S.M. Allan, et al.; Nat. Rev. Immunol. 5, 629 (2005) Abstract
[3] Molecular characterization of NF-HEV, a nuclear factor preferentially expressed in human high endothelial venules: E.S. Baekkevold, et al. ; Am. J. Pathol. 163, 69 (2003) Abstract
[4] IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines: J. Schmitz, et al.; Immunity 23, 479 (2005) Abstract
[5] ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance: E.K. Brint, et al. ; Nat. Immunol. 5, 373 (2005) Abstract
[6] Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases: F. Martinon & J. Tschopp; Cell 117, 561 (2004) Abstract

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Interleukin-33 [IL-33] & Receptors / Related Products • ELISA Sets

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