ALEXIS Biochemicals: IL-35 (human):Fc (human) (recombinant)

ALX-522-140

Revised 08-Aug-08

IL-35 (human):Fc (human) (recombinant)
SYNONYMS	Interleukin-35 (human):Fc (human) (recombinant) EBI3 (human):IL-12A (human):Fc (human) (recombinant) IL-27B (human):CLMF p35 (human):Fc (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	Interleukins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-140-C010		10 µg	360.00 USD

Product Specification

MW:	~85kDa
SOURCE/HOST:	Produced in HEK 293 cells. Human EBI3 (aa 21-229) linked to human IL-12A (aa 23-219) via the linker GGGSGGGSGGGSGGGS is fused at the C-terminus to the Fc portion of human IgG1 and a Flag^®-tag.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Lonza).
RECONSTITUTION:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
BIOLOGICAL ACTIVITY:	Inhibits human mononuclear cells proliferation.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Description

IL-35 (interleukin-35) is a heterodimeric cytokine from the IL-12 family composed of EBI3 (Epstein-Barr virus-induced gene 3 protein; EBV-induced gene 3 protein; IL-27B; IL-27 subunit β) and IL-12A (IL-12 subunit p35) involved in T cell regulation. IL-35 is highly expressed in regulatory T cell (Treg) but not in effector or naive T cells. IL-35 is required for the suppressive function of T-reg in vitro and in vivo.

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link Q14213: IL-27B (human)
Swiss-Prot link P29459: IL-12A (human)

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

The inhibitory cytokine IL-35 contributes to regulatory T-cell function: L.W. Collison, et al. ; Nature 450, 566 (2007) Abstract

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