Advanced Search
© 2008 Alexis Corporation
You are here:
Product Lines
>
Cell Death / Apoptosis / Autophagy
>
Caspases / Related Products
> Caspase Substrates
Toolbar - View Selection
Current View:
Price List
Brief
Detail
Search Results
Current Sort:
Sort by Product Name
Sort by Product ID
Sort by Result Relevance
Sort by Class
Show:
20 Products
50 Products
100 Products
All Products
Show Selected Products Only
Show
Products Only
Items
1-20
of 44
Page 1
of 3
Select Page:
1
2
3
>>
ALX-260-094
Revised 09-May-06
D2R (high purity)
SYNONYMS
(L-Asp)
2
rhodamine 110 (high purity) . TFA salt
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-094-M001
1 mg
120.00 USD
ALX-260-094-5001
5x1 mg
420.00 USD
Product Specification
FORMULA:
C
28
H
24
N
4
O
9
. CF
3
COOH
MW:
560.5 . 114.0
PURITY:
≥98%
APPEARANCE:
Off-white to orange powder.
SOLUBILITY:
Soluble in DMSO.
RECONSTITUTION:
Reconstitute in DMSO followed by the addition of ethanol at a ratio 1:1.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Aliquots in DMSO:ethanol (1:1) are stable for at least 1 month when stored at -20°C and stable for at least 6 months when stored at -80°C. Use high purity reagents only as the dye is instable in water. Discard surplus aqueous solutions.
HANDLING:
After reconstitution, prepare aliquots and store at -20°C. Protect from light.
Product Description
Substrate for detection of intracellular caspase activation by flow cytometry and fluorescence microscopy after apoptosis induction in intact hematopoietic cell lines. For detailed application protocols see [2].
Product Specific Literature References
[1]
Rhodamine 110-linked amino acids and peptides as substrates to measure caspase activity upon apoptosis induction in intact cells:
H. Hug, et al.; Biochemistry
38
, 13906 (1999)
Abstract
[2]
Fluorogenic substrates as detectors of caspase activity during natural killer cell-induced apoptosis:
M. Los, et al.; Methods Mol. Biol.
121
, 155 (2000)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
D
2
R can be used to detect caspase activity (predominantly caspase-3) in intact cells by flow cytometry [1], fluorescence microscopy as well as in cell extracts followed by analysis with a fluorometer (λex: 488nm/λem 550nm). Free (cleaved) Rhodamine 110 emits light both in the FL1 and FL2 channel. Dual staining is possible by employing the FL3 channel for flow cytometry. At least 160 assays can be performed (100µl sample volume (e.g. whole blood sample) and 100µM D
2
R) [2]. Optimal substrate concentrations for other applications range from 25-50µM D
2
R and have to be determined empirically for the particular cell system.
ALX-260-023
Revised 04-Jan-07
Mca-YVADAPK(Dnp)-OH
SYNONYMS
Caspase-1 Substrate (Fluorogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-023-M001
1 mg
390.00 USD
Product Specification
SEQUENCE:
Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-OH (Mca = (7-Methoxycoumarin-4-yl)acetyl; Dnp = Dinitrophenyl)
FORMULA:
C
53
H
64
N
10
O
19
MW:
1145.1
PURITY:
≥95% (HPLC)
APPEARANCE:
White to yellow powder.
SOLUBILITY:
Soluble in DMSO or 80% acetic acid (1mg/ml, with agitation).
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Description
Specific, highly fluorescent substrate for caspase-1. Also substrate for caspase-4.
Product Specific Literature References
Sequential activation of ICE-like and CPP32-like proteases during Fas- mediated apoptosis:
M. Enari, et al.; Nature
380
, 723 (1996)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Mca has an excitation maximum of 325nm and an emission maximum of 392nm.
ALX-260-024
Revised 17-Jan-06
Ac-YVAD-AMC
SYNONYMS
Caspase-1 Substrate (Fluorogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-024-M001
1 mg
37.00 USD
ALX-260-024-M005
5 mg
98.00 USD
Product Specification
SEQUENCE:
Ac-Tyr-Val-Ala-Asp-AMC (AMC = 7-Amino-4-methylcoumarin)
FORMULA:
C
33
H
39
N
5
O
10
MW:
665.7
PEPTIDE CONTENT:
75-95%
PURITY:
≥99% (HPLC)
APPEARANCE:
White to off-white powder.
SOLUBILITY:
Soluble in DMSO. Dilute with distilled water or buffer, pH 7.0-7.5 (e. g. TRIS, HEPES).
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Keep cool and dry.
Product Description
Fluorogenic substrate for caspase-1.
Product Specific Literature References
A novel heterodimeric cysteine protease is required for interleukin-1 β processing in monocytes:
N.A. Thornberry, et al.; Nature
356
, 768 (1992)
Abstract
Interleukin-1 β converting enzyme:
N.A. Thornberry; Meth. Enzymol.
244
, 615 (1994)
Abstract
Crystal structure of the cysteine protease interleukin-1 β-converting enzyme: a (p20/p10)2 homodimer:
N.P.C. Walker, et al.; Cell
78
, 343 (1994)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
AMC has an excitation maximum of 380nm and an emission maximum of 460nm.
ALX-260-026
Revised 24-Feb-05
Ac-YVAD-pNA
SYNONYMS
Caspase-1 Substrate (Chromogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-026-M001
1 mg
32.00 USD
ALX-260-026-M005
5 mg
68.00 USD
Product Specification
SEQUENCE:
Ac-Tyr-Val-Ala-Asp-pNA (pNA=p-Nitroaniline)
FORMULA:
C
29
H
36
N
6
O
10
MW:
628.6
PEPTIDE CONTENT:
75-95%
PURITY:
≥99% (HPLC)
APPEARANCE:
White to off-white powder.
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in DMSO. Dilute with buffer pH 7.5.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Keep cool and dry.
Product Description
Chromogenic substrate for caspase-1.
Product Specific Literature References
A novel heterodimeric cysteine protease is required for interleukin-1 β processing in monocytes:
N.A. Thornberry, et al.; Nature
356
, 768 (1992)
Abstract
Peptidic p-nitroanilide substrates of interleukin-1 β-converting enzyme:
L.A. Reiter; Int. J. Pept. Protein Res.
43
, 87 (1994)
Abstract
Interleukin-1 β converting enzyme:
N.A. Thornberry; Meth. Enzymol.
244
, 615 (1994)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
λmax of pNA is 400nm.
ALX-260-031
Revised 02-Mar-06
Ac-DEVD-AMC
SYNONYMS
Caspase-3 Substrate (Fluorogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-031-M001
1 mg
40.00 USD
ALX-260-031-M005
5 mg
115.00 USD
Product Specification
SEQUENCE:
Ac-Asp-Glu-Val-Asp-AMC (AMC = 7-Amino-4-methylcoumarin)
FORMULA:
C
30
H
37
N
5
O
13
MW:
675.7
PEPTIDE CONTENT:
70-95%
PURITY:
≥97% (HPLC)
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in DMSO. Dilute with buffer, pH 7.5.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from light.
Product Description
Fluorogenic substrate for caspase-3. Also a substrate for caspase-1, -4, -7 and -8. The peptide sequence is based on the PARP cleavage site Asp
216
for caspase-3.
Product Specific Literature References
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis:
D.W. Nicholson, et al.; Nature
376
, 37 (1995)
Abstract
Purification and catalytic properties of human caspase family members:
M. Garcia-Calvo, et al.; Cell Death Differ.
6
, 362 (1999)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
AMC has an excitation maximum of 380nm and an emission maximum of 460nm.
ALX-260-032
Revised 27-Apr-05
Ac-DEVD-AFC
SYNONYMS
Caspase-3 Substrate (Fluorogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-032-M001
1 mg
47.00 USD
ALX-260-032-M005
5 mg
138.00 USD
Product Specification
SEQUENCE:
Ac-Asp-Glu-Val-Asp-AFC (AFC = 7-Amino-4-trifluoromethylcoumarin)
FORMULA:
C
30
H
34
F
3
N
5
O
13
MW:
729.6
PEPTIDE CONTENT:
65-95%
PURITY:
≥96% (HPLC)
APPEARANCE:
White to off-white powder.
SOLUBILITY:
Soluble in dimethyl formamide, DMSO or methanol; slightly soluble in water (0.4mg/ml).
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Keep cool and dry.
Product Description
Fluorogenic substrate for caspase-3. Also a substrate for caspase-1, -4 and -7. This product is similar to Ac-DEVD-AMC (Prod. No.
ALX-260-031
) but the AFC fluorophore has a greater Stokes’ shift upon cleavage. The peptide sequence is based on the PARP cleavage site Asp
216
for caspase-3.
Product Specific Literature References
BAX-induced cell death may not require interleukin 1 β-converting enzyme-like proteases:
J. Xiang, et al.; Proc. Natl. Acad. Sci. USA
93
, 14559 (1996)
Abstract
Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver:
J.M. Chandler, et al.; J. Biol. Chem.
273
, 10815 (1998)
Abstract
;
Full Text
Caspase-7 is activated during lovastatin-induced apoptosis of the prostate cancer cell line LNCaP:
M. Marcelli, et al.; Cancer Res.
58
, 76 (1998)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
AFC has an excitation maximum of 400nm and an emission maximum of 505nm.
ALX-260-033
Revised 10-Sep-08
Ac-DEVD-pNA
SYNONYMS
Caspase-3 Substrate (Chromogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-033-M001
1 mg
27.00 USD
ALX-260-033-M005
5 mg
73.00 USD
Product Specification
SEQUENCE:
Ac-Asp-Glu-Val-Asp-pNA (pNA = p-Nitroaniline)
FORMULA:
C
26
H
34
N
6
O
13
MW:
638.6
PEPTIDE CONTENT:
70-95%
PURITY:
≥97% (HPLC)
APPEARANCE:
White to off-white powder.
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in DMSO or buffer, pH 7.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Always use fresh solutions.
HANDLING:
Keep cool and dry.
Product Description
Chromogenic substrate for caspase-3. Also a substrate for caspase-1, -4 and -7.
Product Specific Literature References
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis:
D.W. Nicholson, et al.; Nature
376
, 37 (1995)
Abstract
Activation of the CPP32 protease in apoptosis induced by 1-beta-D- arabinofuranosylcytosine and other DNA-damaging agents:
R. Datta, et al.; Blood
88
, 1936 (1996)
Abstract
Substrate specificities of caspase family proteases:
R.V. Talanian, et al.; J. Biol. Chem.
272
, 9677 (1997)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
λmax of pNA is 400nm.
ALX-260-035
Revised 10-Feb-05
Z-YVAD-AFC
SYNONYMS
Caspase-1 Substrate (Fluorogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-035-M001
1 mg
40.00 USD
ALX-260-035-M005
5 mg
148.00 USD
Product Specification
SEQUENCE:
Z-Tyr-Val-Ala-Asp-AFC (AFC = 7-Amido-4-trifluoromethylcoumarin)
FORMULA:
C
39
H
40
N
5
O
11
F
3
MW:
811.8
PURITY:
≥95% (HPLC).
Single spot (TLC)
APPEARANCE:
White to off-white powder.
SOLUBILITY:
Soluble in DMSO, DMF or methanol; insoluble in water.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
Product Description
Fluorogenic substrate for caspase-1. AFC has an excitation maximum of 400nm and an emission maximum of 505nm. This product is similar to Ac-YVAD-AMC, Prod. No.
ALX-260-024
, but the AFC fluorophore has a greater Stokes' shift upon cleavage and the N-terminal is blocked by a benzyloxycarbonyl moiety, which increases cell permeability.
Product Specific Literature References
A novel heterodimeric cysteine protease is required for interleukin-1 β processing in monocytes:
N.A. Thornberry, et al.; Nature
356
, 768 (1992)
Abstract
Peptidic p-nitroanilide substrates of interleukin-1 β-converting enzyme:
L.A. Reiter; Int. J. Pept. Protein Res.
43
, 87 (1994)
Abstract
N.A. Thornberry; Meth. Enzymol.
244
, 615 (1994)
Abstract
N.P. Walker, et al.; Cell
78
, 343 (1994)
Abstract
Activation of multiple interleukin-1beta converting enzyme homologues in cytosol and nuclei of HL-60 cells during etoposide-induced apoptosis:
L.M. Martins, et al.; J. Biol. Chem.
272
, 7421 (1997)
Abstract
;
Full Text
ALX-260-042
Revised 02-Oct-07
Ac-IETD-AMC
SYNONYMS
Caspase-8 Substrate (Fluorogenic)
Granzyme B Substrate (Fluorogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-042-M001
1 mg
42.00 USD
ALX-260-042-M005
5 mg
163.00 USD
Product Specification
SEQUENCE:
Ac-Ile-Glu-Thr-Asp-AMC (AMC=7-Amino-4-methylcoumarin)
FORMULA:
C
31
H
41
N
5
O
12
MW:
675.7
PEPTIDE CONTENT:
70-90%
PURITY:
≥98% (HPLC)
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in DMSO. Dilute with buffer, pH 7.5.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
Product Description
Fluorogenic substrate for caspase-8 and granzyme B. Also a substrate for caspase-10.
Product Specific Literature References
Bid truncation, bid/bax targeting to the mitochondria, and caspase activation associated with neutrophil apoptosis are inhibited by granulocyte colony-stimulating factor
:
N.A. Maianski, et al.; J. Immunol.
172
, 7024 (2004)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
AMC has an excitation maximum of 380nm and an emission maximum of 460nm.
Further Categories Containing This Product:
Granzyme Substrates
ALX-260-044
Revised 05-Apr-05
Z-IETD-pNA
SYNONYMS
Caspase-8 Substrate (Chromogenic)
Granzyme B Substrate (Chromogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-044-M001
1 mg
22.00 USD
ALX-260-044-M005
5 mg
83.00 USD
Product Specification
SEQUENCE:
Z-Ile-Glu-Thr-Asp-pNA (pNA = p-Nitroaniline)
FORMULA:
C
33
H
42
N
6
O
13
MW:
730.7
PEPTIDE CONTENT:
70-90%
PURITY:
≥99% (HPLC)
APPEARANCE:
White powder.
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in water.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Keep cool and dry.
Product Description
Fluorogenic substrate for caspase-8 and granzyme B with increased cell permeability. Also substrate for caspase-10.
General Information
BACKGROUND/TECHNICAL INFORMATION
λmax of pNA is 400nm.
Further Categories Containing This Product:
Granzyme Substrates
ALX-260-045
Revised 15-Dec-05
Ac-IETD-pNA
SYNONYMS
Caspase-8 Substrate (Chromogenic)
Granzyme B Substrate (Chromogenic)
PRODUCT LINE
