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ALX-804-137
Revised 07-Dec-05
Monoclonal Antibody to A20 (human) (8E8.38)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
TLRs Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-137-C050
50 µg
360.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
8E8.38
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Zinc finger domain corresponding to aa 375-537 of human A20.
SPECIFICITY:
Recognizes human A20.
APPLICATION:
Western Blot (1:1'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored as recommended.
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Description
A20 is a zinc finger protein that renders cells resistant to apoptosis. A20-deficient mice develop severe inflammation and are hyper-responsive to LPS. S.M. O'Reilly & P.N. Moynagh have shown that full length A20 inhibits activation of NF-kB and AP-1 by TLR4 [1]. A20 associates with A20-binding inhibitor of NF-kB (ABIN)-1 and -2 [2-4], which interfere with NF-kB activation upstream of the IKK complex.
Product Specific Literature References
A20 inhibits NF-kappa B activation downstream of multiple Map3 kinases and interacts with the I kappa B signalosome
:
F.S. Zetoune, et al.; Cytokine
15
, 282 (2001)
Abstract
General Information
MANUFACTURER
Manufactured by Apotech Corporation.
General Literature References
[1]
Regulation of Toll-like receptor 4 signalling by A20 zinc finger protein
:
S.M. O'Reilly and P.N. Moynagh; Biochem. Biophys. Res. Commun.
3303
, 586 (2003)
Abstract
[2]
The zinc finger protein A20 inhibits TNF-induced NF-kappaB-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-kappaB-inhibiting protein ABIN
:
K. Heyninck, et al.; J. Cell Biol.
145
, 1471 (1999)
Abstract
;
Full Text
[3]
Identification of a novel A20-binding inhibitor of nuclear factor-kappa B activation termed ABIN-2
:
S. Van Huffel, et al.; J Biol Chem
276
, 30216 (2001)
Abstract
;
Full Text
[4]
Structure-function analysis of the A20-binding inhibitor of NF-kappa B activation, ABIN-1
:
K. Heyninck, et al.; FEBS Lett.
536
, 135 (2003)
Abstract
Further Categories Containing This Product:
NF-kB Pathway Other Products
•
Cell Death / Apoptosis / Autophagy Other Products
•
Monoclonal Antibodies
ALX-804-134
Revised 01-Mar-07
Monoclonal Antibody to Bcl-10 (Carmen-1)
SYNONYMS
anti-CARMEN MAb (Carmen-1)
anti-CIPER MAb (Carmen-1)
anti-CLAP MAb (Carmen-1)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Caspase Recruitment Domain [CARD] Proteins/Related Products
Ordering Information
Product Numbers:
Format:
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ALX-804-134-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
Carmen-1
ISOTYPE:
Rat IgM
SOURCE/HOST:
Purified from concentrated hybridoma cell culture supernatant.
CONCENTRATION:
1mg/ml
PURITY:
>95% (SDS-PAGE)
PURITY DETAIL:
Purified.
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human Bcl-10.
SPECIFICITY:
Recognizes human and mouse Bcl-10.
APPLICATION:
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
USE/STABILITY:
Stable for at least 6 months after receipt when stored undiluted at +4°C.
HANDLING:
Do not freeze/thaw.
Product Images
Please click on thumbnails to enlarge.
General Information
MANUFACTURER
Manufactured by Apotech Corporation.
Further Categories Containing This Product:
NF-kB Pathway Other Products
•
Innate Immunity Other Products
•
Monoclonal Antibodies
ALX-804-847
Revised 19-Jun-08
Monoclonal Antibody to Cardif (human) (Adri-1)
SYNONYMS
anti-CARD Adapter Inducing Interferon-β (human) MAb (Adri-1)
anti-IPS-1 Cardif (human) MAb (Adri-1)
anti-Interferon-β Promoter Stimulator Protein 1 (human) MAb (Adri-1)
anti-MAVS (human) MAb (Adri-1)
anti-Mitochondrial Antiviral Signalling Protein (human) MAb (Adri-1)
anti-VISA (human) MAb (Adri-1)
anti-Virus-induced Signalling Adapter (human) MAb (Adri-1)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
Antiviral Signalling/Related Products
Ordering Information
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Format:
Size:
Unit Price:
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ALX-804-847-C100
100 µg
370.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
Adri-1
ISOTYPE:
Mouse IgG2b
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450).
SPECIFICITY:
Recognizes human Cardif.
APPLICATION:
Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections)
Immunoprecipitation (1:200)
Western Blot (1:1’000)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
TRADD Protein Is an Essential Component of the RIG-like Helicase Antiviral Pathway:
M.C. Michallet, et al.; Immunity
28
, 651 (2008)
Abstract
General Information
RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons.
Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.
MANUFACTURER
Manufactured by Apotech Corporation.
General Literature References
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus:
E. Meylan, et al.; Nature
437
, 1167 (2005)
Abstract
VISA is an adapter protein required for virus-triggered IFN-beta signaling:
L.G. Xu, et al.; Mol. Cell
19
, 727 (2005)
Abstract
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction:
T. Kawai, et al.; Nat. Immunol.
6
, 981 (2005)
Abstract
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3:
R.B. Seth, et al.; Cell
122
, 669 (2005)
Abstract
CARD games between virus and host get a new player:
C.L. Johnson and M. Gale, Jr.; Trends Immunol.
27
, 1 (2006)
Abstract
Further Categories Containing This Product:
NF-kB Pathway Other Products
•
Caspase Recruitment Domain [CARD] Proteins/Related Products
•
Polyclonal Antibodies
ALX-804-530
Revised 14-Aug-07
Monoclonal Antibody to Caspase-1 (mouse) (1H11)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Antibodies
Ordering Information
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Format:
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ALX-804-530-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
1H11
ISOTYPE:
Rat IgG1
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. Protein G sepharose purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN:
p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T
206
ALEMVKEVKEFAAC
220
) of mouse caspase-1.
SPECIFICITY:
Recognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45kDa by Western blot. Does not cross-react with mouse caspase-11 or -12.
APPLICATION:
ELISA
Flow Cytometry
Western Blot
Other applications not tested.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death:
V.S. Marsden, et al.; Blood
107
, 1872 (2006)
Abstract
;
Full Text
General Information
Capases are a highly conserved family of cysteine proteases and are synthesized as inactive zymogens, which are cleaved at Asp-x sites during apoptosis, generating large and small subunits that heterodimerize to form the active enzyme. Caspase-1 is a member of the ICE/CED-3 family of caspases and is detected in the pro-form as a protein of 45 kDa.
Caspase-1 generates the bioactive form of the pro-inflammatory cytokine IL-1β from its biologically inactive precursor. Overexpression of caspase-1 in cells induces apoptosis, which can be inhibited by cytokine response modifier A (CrmA). Caspase-1 mRNA is predominantly expressed in the spleen and lung and to a lesser extent in skeletal muscle, kidney, testis and heart and within these tissues macrophages preferentially express caspase-1. Caspase-1 deficient mice have no gross abnormalities, however, macrophages from caspase-1-deficient mice are defective in IL-β processing and release.
General Literature References
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins
:
L.A. O'Reilly, et al.; Biotechniques
25
, 824 (1998), Methodology
Abstract
Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9
:
L.A. O'Reilly, et al.; Cell Death Differ.
9
, 832 (2002), Methodology
Abstract
Further Categories Containing This Product:
Inflammasome
•
Monoclonal Antibodies
ALX-804-531
Revised 10-Mar-06
Monoclonal Antibody to Caspase-1 (mouse) (4G8)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-531-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
4G8
ISOTYPE:
Rat IgG1
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. Protein G sepharose purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN:
p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T
206
ALEMVKEVKEFAAC
220
) of mouse caspase-1.
SPECIFICITY:
Recognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45kDa by Western blot. Does not cross-react with mouse caspase-11 or -12.
APPLICATION:
ELISA
Flow Cytometry
Western Blot
Other applications not tested.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
Product Images
Please click on thumbnails to enlarge.
General Information
Capases are a highly conserved family of cysteine proteases and are synthesized as inactive zymogens, which are cleaved at Asp-x sites during apoptosis, generating large and small subunits that heterodimerize to form the active enzyme. Caspase-1 is a member of the ICE/CED-3 family of caspases and is detected in the pro-form as a protein of 45 kDa.
Caspase-1 generates the bioactive form of the pro-inflammatory cytokine IL-1β from its biologically inactive precursor. Overexpression of caspase-1 in cells induces apoptosis, which can be inhibited by cytokine response modifier A (CrmA). Caspase-1 mRNA is predominantly expressed in the spleen and lung and to a lesser extent in skeletal muscle, kidney, testis and heart and within these tissues macrophages preferentially express caspase-1. Caspase-1 deficient mice have no gross abnormalities, however, macrophages from caspase-1-deficient mice are defective in IL-β processing and release.
General Literature References
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins
:
L.A. O'Reilly, et al.; Biotechniques
25
, 824 (1998), Methodology
Abstract
Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9
:
L.A. O'Reilly, et al.; Cell Death Differ.
9
, 832 (2002), Methodology
Abstract
Further Categories Containing This Product:
Inflammasome
•
Monoclonal Antibodies
ALX-804-209
Revised 27-May-08
Monoclonal Antibody to IκBα (6A920)
SYNONYMS
anti-NF-κB Inhibitor α MAb (6A920)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Inhibitors of kappa B [IkB]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-209-C100
100 µg
293.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
6A920
ISOTYPE:
Mouse IgG1
CONCENTRATION:
0.5mg/ml
PURITY DETAIL:
Protein G affinity-purified.
FORMULATION:
Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN:
Recombinant human IκBα
(aa 32-391).
SPECIFICITY:
Recognizes both phosphorylated and non-phosphorylated forms of human and mouse IκBα. Detects a band of ~40kDa by Western blot.
APPLICATION:
Immunoprecipitation (1µg/ml)
Western Blot (1-2µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 6 months when stored at +4°C.
Product Images
Please click on thumbnails to enlarge.
General Literature References
Two waves of nuclear factor kappaB recruitment to target promoters
:
S. Saccani, et al.; J. Exp. Med.
193
, 1351 (2001)
Abstract
;
Full Text
p38-Dependent marking of inflammatory genes for increased NF-kappa B recruitment
:
S. Saccani, et al.; Nat. Immunol.
3
, 69 (2002)
Abstract
Modulation of NF-kappaB activity by exchange of dimers
:
S. Saccani, et al.; Mol. Cell
11
, 1563 (2003)
Abstract
PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock
:
T. Ikezoe, et al.; Mol. Pharmacol.
64
, 1521 (2003)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-440
Revised 19-Sep-05
Monoclonal Antibody to IκBα (cleaved) (human) (5D1623)
SYNONYMS
anti-NF-κB Inhibitor α (cleaved) (human) MAb (5D1623)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Inhibitors of kappa B [IkB]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-440-C100
100 µg
319.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
5D1623
ISOTYPE:
Mouse IgG1
CONCENTRATION:
0.5mg/ml
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to the caspase-3 mediated cleavage site of IκBα (NF-κB Inhibitor α).
SPECIFICITY:
Recognizes caspase-3 cleaved human IκBα.
APPLICATION:
Western Blot (1µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 6 months when stored at +4°C.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-235
Revised 31-Oct-06
Monoclonal Antibody to IκBα (human) (phosphorylated) (pSer32/36) (39A1431)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Inhibitors of kappa B [IkB]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-235-C100
100 µg
319.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
39A1431
ISOTYPE:
Mouse IgG1
CONCENTRATION:
0.5mg/ml
PURITY DETAIL:
Protein G affinity-purified.
FORMULATION:
Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to a portion of human IκBα phosphorylated at Ser
32
and Ser
36
.
SPECIFICITY:
Recognizes phosphorylated human IκBα. Detects a band of ~40kDa by Western blot. Does not cross-react with non-phosphorylated IκBα
APPLICATION:
ELISA
Immunoprecipitation (1-2µg/ml)
Western Blot (1-2µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 6 months when stored at +4°C.
Product Images
Please click on thumbnails to enlarge.
Further Categories Containing This Product:
Phospho-specific Antibodies
•
Monoclonal Antibodies
ALX-804-236
Revised 27-Feb-07
Monoclonal Antibody to IKKα (14A231)
SYNONYMS
anti-Inhibitor of Nuclear Factor κ-B Kinase α MAb (14A231)
anti-IKK1 MAb (14A231)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
IkB Kinases [IKKs]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-236-C100
100 µg
293.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Monkey
CLONE:
14A231
ISOTYPE:
Mouse IgG1
CONCENTRATION:
0.5mg/ml
PURITY DETAIL:
Protein G affinity-purified.
FORMULATION:
Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN:
Recombinant human IKKα (inhibitor of NF-κB kinase α; IKK1).
SPECIFICITY:
Recognizes human, mouse and monkey IKKα. Detects a band of ~85kDa by Western blot.
APPLICATION:
Immunoprecipitation (1-2µg/ml)
Western Blot (1µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 6 months when stored at +4°C.
Product Specific Literature References
IFN-alpha suppresses activation of nuclear transcription factors NF-kappa B and activator protein 1 and potentiates TNF-induced apoptosis:
S.K. Manna, et al.; J. Immunol.
165
, 4927 (2000)
Abstract
;
Full Text
KKalpha provides an essential link between RANK signaling and cyclin D1 expression during mammary gland development
:
Y. Cao, et al.; Cell
107
, 763 (2001)
Abstract
Tumor suppressor MMAC/PTEN inhibits cytokine-induced NFkappaB activation without interfering with the IkappaB degradation pathway
:
D. Koul, et al.; J. Biol. Chem.
276
, 11402 (2001)
Abstract
;
Full Text
Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation
:
S. Majumdar & B.B. Aggarwal; J. Immunol.
167
, 2911 (2001)
Abstract
;
Full Text
Complete reconstitution of human IkappaB kinase (IKK) complex in yeast. Assessment of its stoichiometry and the role of IKKgamma on the complex activity in the absence of stimulation
:
B.S. Miller & E. Zandi; J. Biol. Chem.
276
, 36320 (2001)
Abstract
;
Full Text
NEMO trimerizes through its coiled-coil C-terminal domain
:
F. Agou, et al.; J. Biol. Chem.
277
, 17464 (2002)
Abstract
;
Full Text
The lymphotoxin-beta receptor induces different patterns of gene expression via two NF-kappaB pathways
:
E. Dejardin, et al.; Immunity
17
, 525 (2002)
Abstract
Thalidomide suppresses NF-kappa B activation induced by TNF and H2O2, but not that activated by ceramide, lipopolysaccharides, or phorbol ester:
S. Majumdar, et al.; J. Immunol.
168
, 2644 (2002)
Abstract
;
Full Text
The carboxyl-terminal region of IkappaB kinase gamma (IKKgamma) is required for full IKK activation
:
C. Makris, et al.; Mol. Cell. Biol.
22
, 6573 (2002)
Abstract
;
Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-237
Revised 27-Feb-07
Monoclonal Antibody to IKKβ (human) (10AG2)
