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ALX-260-023
Revised 04-Jan-07
Mca-YVADAPK(Dnp)-OH
SYNONYMS
Caspase-1 Substrate (Fluorogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-260-023-M001
1 mg
390.00 USD
Product Specification
SEQUENCE:
Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-OH (Mca = (7-Methoxycoumarin-4-yl)acetyl; Dnp = Dinitrophenyl)
FORMULA:
C
53
H
64
N
10
O
19
MW:
1145.1
PURITY:
≥95% (HPLC)
APPEARANCE:
White to yellow powder.
SOLUBILITY:
Soluble in DMSO or 80% acetic acid (1mg/ml, with agitation).
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Description
Specific, highly fluorescent substrate for caspase-1. Also substrate for caspase-4.
Product Specific Literature References
Sequential activation of ICE-like and CPP32-like proteases during Fas- mediated apoptosis:
M. Enari, et al.; Nature
380
, 723 (1996)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Mca has an excitation maximum of 325nm and an emission maximum of 392nm.
ALX-260-024
Revised 17-Jan-06
Ac-YVAD-AMC
SYNONYMS
Caspase-1 Substrate (Fluorogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-024-M001
1 mg
37.00 USD
ALX-260-024-M005
5 mg
98.00 USD
Product Specification
SEQUENCE:
Ac-Tyr-Val-Ala-Asp-AMC (AMC = 7-Amino-4-methylcoumarin)
FORMULA:
C
33
H
39
N
5
O
10
MW:
665.7
PEPTIDE CONTENT:
75-95%
PURITY:
≥99% (HPLC)
APPEARANCE:
White to off-white powder.
SOLUBILITY:
Soluble in DMSO. Dilute with distilled water or buffer, pH 7.0-7.5 (e. g. TRIS, HEPES).
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Keep cool and dry.
Product Description
Fluorogenic substrate for caspase-1.
Product Specific Literature References
A novel heterodimeric cysteine protease is required for interleukin-1 β processing in monocytes:
N.A. Thornberry, et al.; Nature
356
, 768 (1992)
Abstract
Interleukin-1 β converting enzyme:
N.A. Thornberry; Meth. Enzymol.
244
, 615 (1994)
Abstract
Crystal structure of the cysteine protease interleukin-1 β-converting enzyme: a (p20/p10)2 homodimer:
N.P.C. Walker, et al.; Cell
78
, 343 (1994)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
AMC has an excitation maximum of 380nm and an emission maximum of 460nm.
ALX-260-026
Revised 24-Feb-05
Ac-YVAD-pNA
SYNONYMS
Caspase-1 Substrate (Chromogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-026-M001
1 mg
32.00 USD
ALX-260-026-M005
5 mg
68.00 USD
Product Specification
SEQUENCE:
Ac-Tyr-Val-Ala-Asp-pNA (pNA=p-Nitroaniline)
FORMULA:
C
29
H
36
N
6
O
10
MW:
628.6
PEPTIDE CONTENT:
75-95%
PURITY:
≥99% (HPLC)
APPEARANCE:
White to off-white powder.
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in DMSO. Dilute with buffer pH 7.5.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Keep cool and dry.
Product Description
Chromogenic substrate for caspase-1.
Product Specific Literature References
A novel heterodimeric cysteine protease is required for interleukin-1 β processing in monocytes:
N.A. Thornberry, et al.; Nature
356
, 768 (1992)
Abstract
Peptidic p-nitroanilide substrates of interleukin-1 β-converting enzyme:
L.A. Reiter; Int. J. Pept. Protein Res.
43
, 87 (1994)
Abstract
Interleukin-1 β converting enzyme:
N.A. Thornberry; Meth. Enzymol.
244
, 615 (1994)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
λmax of pNA is 400nm.
ALX-260-031
Revised 02-Mar-06
Ac-DEVD-AMC
SYNONYMS
Caspase-3 Substrate (Fluorogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-031-M001
1 mg
40.00 USD
ALX-260-031-M005
5 mg
115.00 USD
Product Specification
SEQUENCE:
Ac-Asp-Glu-Val-Asp-AMC (AMC = 7-Amino-4-methylcoumarin)
FORMULA:
C
30
H
37
N
5
O
13
MW:
675.7
PEPTIDE CONTENT:
70-95%
PURITY:
≥97% (HPLC)
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in DMSO. Dilute with buffer, pH 7.5.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from light.
Product Description
Fluorogenic substrate for caspase-3. Also a substrate for caspase-1, -4, -7 and -8. The peptide sequence is based on the PARP cleavage site Asp
216
for caspase-3.
Product Specific Literature References
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis:
D.W. Nicholson, et al.; Nature
376
, 37 (1995)
Abstract
Purification and catalytic properties of human caspase family members:
M. Garcia-Calvo, et al.; Cell Death Differ.
6
, 362 (1999)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
AMC has an excitation maximum of 380nm and an emission maximum of 460nm.
ALX-260-032
Revised 27-Apr-05
Ac-DEVD-AFC
SYNONYMS
Caspase-3 Substrate (Fluorogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-032-M001
1 mg
47.00 USD
ALX-260-032-M005
5 mg
138.00 USD
Product Specification
SEQUENCE:
Ac-Asp-Glu-Val-Asp-AFC (AFC = 7-Amino-4-trifluoromethylcoumarin)
FORMULA:
C
30
H
34
F
3
N
5
O
13
MW:
729.6
PEPTIDE CONTENT:
65-95%
PURITY:
≥96% (HPLC)
APPEARANCE:
White to off-white powder.
SOLUBILITY:
Soluble in dimethyl formamide, DMSO or methanol; slightly soluble in water (0.4mg/ml).
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Keep cool and dry.
Product Description
Fluorogenic substrate for caspase-3. Also a substrate for caspase-1, -4 and -7. This product is similar to Ac-DEVD-AMC (Prod. No.
ALX-260-031
) but the AFC fluorophore has a greater Stokes’ shift upon cleavage. The peptide sequence is based on the PARP cleavage site Asp
216
for caspase-3.
Product Specific Literature References
BAX-induced cell death may not require interleukin 1 β-converting enzyme-like proteases:
J. Xiang, et al.; Proc. Natl. Acad. Sci. USA
93
, 14559 (1996)
Abstract
Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver:
J.M. Chandler, et al.; J. Biol. Chem.
273
, 10815 (1998)
Abstract
;
Full Text
Caspase-7 is activated during lovastatin-induced apoptosis of the prostate cancer cell line LNCaP:
M. Marcelli, et al.; Cancer Res.
58
, 76 (1998)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
AFC has an excitation maximum of 400nm and an emission maximum of 505nm.
ALX-260-033
Revised 15-Nov-06
Ac-DEVD-pNA
SYNONYMS
Caspase-3 Substrate (Chromogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-033-M001
1 mg
27.00 USD
ALX-260-033-M005
5 mg
73.00 USD
Product Specification
SEQUENCE:
Ac-Asp-Glu-Val-Asp-pNA (pNA = p-Nitroaniline)
FORMULA:
C
26
H
34
N
6
O
13
MW:
638.6
PEPTIDE CONTENT:
70-95%
PURITY:
≥97% (HPLC)
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in DMSO or buffer, pH 7.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Always use fresh solutions.
HANDLING:
Keep cool and dry.
Product Description
Chromogenic substrate for caspase-3. Also a substrate for caspase-1, -4 and -7.
Product Specific Literature References
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis:
D.W. Nicholson, et al.; Nature
376
, 37 (1995)
Abstract
Activation of the CPP32 protease in apoptosis induced by 1-beta-D- arabinofuranosylcytosine and other DNA-damaging agents:
R. Datta, et al.; Blood
88
, 1936 (1996)
Abstract
Substrate specificities of caspase family proteases:
R.V. Talanian, et al.; J. Biol. Chem.
272
, 9677 (1997)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
λmax of pNA is 400nm.
ALX-260-035
Revised 10-Feb-05
Z-YVAD-AFC
SYNONYMS
Caspase-1 Substrate (Fluorogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-035-M001
1 mg
40.00 USD
ALX-260-035-M005
5 mg
148.00 USD
Product Specification
SEQUENCE:
Z-Tyr-Val-Ala-Asp-AFC (AFC = 7-Amido-4-trifluoromethylcoumarin)
FORMULA:
C
39
H
40
N
5
O
11
F
3
MW:
811.8
PURITY:
≥95% (HPLC).
Single spot (TLC)
APPEARANCE:
White to off-white powder.
SOLUBILITY:
Soluble in DMSO, DMF or methanol; insoluble in water.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
Product Description
Fluorogenic substrate for caspase-1. AFC has an excitation maximum of 400nm and an emission maximum of 505nm. This product is similar to Ac-YVAD-AMC, Prod. No.
ALX-260-024
, but the AFC fluorophore has a greater Stokes' shift upon cleavage and the N-terminal is blocked by a benzyloxycarbonyl moiety, which increases cell permeability.
Product Specific Literature References
A novel heterodimeric cysteine protease is required for interleukin-1 β processing in monocytes:
N.A. Thornberry, et al.; Nature
356
, 768 (1992)
Abstract
Peptidic p-nitroanilide substrates of interleukin-1 β-converting enzyme:
L.A. Reiter; Int. J. Pept. Protein Res.
43
, 87 (1994)
Abstract
N.A. Thornberry; Meth. Enzymol.
244
, 615 (1994)
Abstract
N.P. Walker, et al.; Cell
78
, 343 (1994)
Abstract
Activation of multiple interleukin-1beta converting enzyme homologues in cytosol and nuclei of HL-60 cells during etoposide-induced apoptosis:
L.M. Martins, et al.; J. Biol. Chem.
272
, 7421 (1997)
Abstract
;
Full Text
ALX-260-042
Revised 02-Oct-07
Ac-IETD-AMC
SYNONYMS
Caspase-8 Substrate (Fluorogenic)
Granzyme B Substrate (Fluorogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-042-M001
1 mg
42.00 USD
ALX-260-042-M005
5 mg
163.00 USD
Product Specification
SEQUENCE:
Ac-Ile-Glu-Thr-Asp-AMC (AMC=7-Amino-4-methylcoumarin)
FORMULA:
C
31
H
41
N
5
O
12
MW:
675.7
PEPTIDE CONTENT:
70-90%
PURITY:
≥98% (HPLC)
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in DMSO. Dilute with buffer, pH 7.5.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
Product Description
Fluorogenic substrate for caspase-8 and granzyme B. Also a substrate for caspase-10.
Product Specific Literature References
Bid truncation, bid/bax targeting to the mitochondria, and caspase activation associated with neutrophil apoptosis are inhibited by granulocyte colony-stimulating factor
:
N.A. Maianski, et al.; J. Immunol.
172
, 7024 (2004)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
AMC has an excitation maximum of 380nm and an emission maximum of 460nm.
Further Categories Containing This Product:
Granzyme Substrates
ALX-260-044
Revised 05-Apr-05
Z-IETD-pNA
SYNONYMS
Caspase-8 Substrate (Chromogenic)
Granzyme B Substrate (Chromogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-044-M001
1 mg
22.00 USD
ALX-260-044-M005
5 mg
83.00 USD
Product Specification
SEQUENCE:
Z-Ile-Glu-Thr-Asp-pNA (pNA = p-Nitroaniline)
FORMULA:
C
33
H
42
N
6
O
13
MW:
730.7
PEPTIDE CONTENT:
70-90%
PURITY:
≥99% (HPLC)
APPEARANCE:
White powder.
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in water.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Keep cool and dry.
Product Description
Fluorogenic substrate for caspase-8 and granzyme B with increased cell permeability. Also substrate for caspase-10.
General Information
BACKGROUND/TECHNICAL INFORMATION
λmax of pNA is 400nm.
Further Categories Containing This Product:
Granzyme Substrates
ALX-260-045
Revised 15-Dec-05
Ac-IETD-pNA
SYNONYMS
Caspase-8 Substrate (Chromogenic)
Granzyme B Substrate (Chromogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-260-045-M001
1 mg
22.00 USD
ALX-260-045-M005
5 mg
83.00 USD
Product Specification
SEQUENCE:
Ac-Ile-Glu-Thr-Asp-pNA (pNA=p-Nitroaniline)
FORMULA:
C
27
H
38
N
6
O
12
MW:
638.6
PEPTIDE CONTENT:
70-90%
PURITY:
98% (HPLC)
APPEARANCE:
White powder.
FORMULATION:
Lyophilized.
SOLUBILITY:
Soluble in DMSO. Dilute with buffer, pH 7.0-7.5.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Keep cool and dry.
Product Description
Chromogenic substrate for caspase-8 and granzyme B. Also a substrate for caspase-10.
Product Specific Literature References
A sequential two-step mechanism for the production of the mature p17:p12 form of caspase-3 in vitro:
Z. Han, et al.; J. Biol. Chem.
272
, 13432 (1997)
Abstract
;
Full Text
Apoptosome-independent pathway for apoptosis. Biochemical analysis of APAF-1 defects and biological outcomes:
C.A. Belmokhtar, et al.; J. Biol. Chem.
278
, 29571 (2003)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
λmax of pNA is 400nm.
Further Categories Containing This Product:
Granzyme Substrates
ALX-260-048
Revised 10-Feb-05
Z-DEVD-pNA
SYNONYMS
Caspase-3 Substrate (Chromogenic)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Substrates
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
