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Lipid Signalling Pathways
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ALX-350-310 Revised 03-Apr-08
3-O-Acetyl-11-keto-β-boswellic acid
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SYNONYMS AKβBA
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Antitumor Reagents
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ALX-350-310-M005   5 mg 130.00 USD Add To Cart
Product Specification
FORMULA: C32H48O5
MW: 512.7
CAS NUMBER: 67416-61-9
SOURCE/HOST: Isolated from Boswellia serrata.
PURITY: ≥99% (HPLC, NMR)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in acetone, dichloromethane, diethyl ether or DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Stock solutions are stable for up to 3 months when stored at -20°C.
HANDLING: Protect from light.
HAZARD: MAY BE CARCINOGENIC. HARMFUL.

Product Description
Shows antitumor and anti-inflammatory activities. Potent non-redox, noncompetitive 5-lipoxygenase and topoisomerase I and IIa inhibitor leading to apoptosis. 10-times more potent than 3-O-acetyl-β-boswellic acid (Prod. No. ALX-350-308). Exhibits in vivo efficacy in tumor growth and inhibition.
Product Specific Literature References
Acetyl-11-keto-beta-boswellic acid induces apoptosis in HL-60 and CCRF-CEM cells and inhibits topoisomerase I: R.F. Hoernlein, et al.; J. Pharmacol. Exp. Ther. 288, 613 (1999) Abstract; Full Text
Boswellic acids activate p42(MAPK) and p38 MAPK and stimulate Ca(2+) mobilization: A. Altmann, et al.; BBRC 290, 185 (2002) Abstract
Coupling of boswellic acid-induced Ca2+ mobilisation and MAPK activation to lipid metabolism and peroxide formation in human leucocytes: A. Altmann, et al.; Br. J. Pharmacol. 141, 223 (2004) Abstract; Full Text
Inhibition of IkappaB kinase activity by acetyl-boswellic acids promotes apoptosis in androgen-independent PC-3 prostate cancer cells in vitro and in vivo: T. Syrovets, et al.; J. Biol. Chem. 280, 6170 (2005) Abstract; Full Text
Boswellic acids: biological actions and molecular targets: D. Poeckel & O. Werz; Curr. Med. Chem. 13, 3359 (2006), Review Abstract
Mechanisms underlying the anti-inflammatory actions of boswellic acid derivatives in experimental colitis: C. Anthoni, et al.; Am. J. Physiol. Gastrointest. Liver Physiol. 290, G1131 (2006) Abstract
Potentiation of antinociceptive effect of NSAIDs by a specific lipooxygenase inhibitor, acetyl 11-keto-beta boswellic acid: M. Bishnoi, et al.; Indian J. Exp. Biol. 44, 128 (2006) Abstract
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Further Categories Containing This Product:
Natural Products - Protein Kinase Inhibitors • Antitumor Agents (Apoptosis Inducers) • NF-kB Pathway Inhibitors • Natural Products - Topoisomerase Inhibitors • Natural Products - Anti-inflammatory Agents • Natural Products - NF-kB Pathway Inhibitors • Natural Products - Apoptosis Inducers & Inhibitors • Lipoxygenases / Related Products
 
 
ALX-270-381 Revised 28-Sep-07
Anacardic acid
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SYNONYMS 2-Hydroxy-6-pentadecylbenzoic acid
6-Pentadecylsalicylic acid
AA
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Histone Acetyl- & Methyl-Transferases / Related Products
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ALX-270-381-M005   5 mg 40.00 USD Add To Cart
ALX-270-381-M025   25 mg 140.00 USD Add To Cart
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Product Specification
FORMULA: C22H36O3
MW: 348.5
MERCK INDEX: 14: 621
SOURCE/HOST: Synthetic.
PURITY: ≥95% (1H-NMR)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in 100% ethanol, methanol, DMSO, dichloromethane or ethylacetate.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
USE/STABILITY: Stock solutions are stable for up to 3 months when stored at -20°C.
HANDLING: Protect from light. After reconstitution, prepare aliquots and store at -20°C. Packaged under inert gas.

Product Description
Cell permeable salicylic acid analog that acts as a potent, non-competitive inhibitor of p300 and PCAF (p300/CBP-associated factor) histone acetyltransferase (HAT) activities (IC50~8.5µM and ~5µM, respectively). Displays a variety of other biological activities, such as antibacterial, antimicrobial, prostaglandin synthase inhibition, tyrosinase, and lipoxygenase inhibition.
Product Specific Literature References
Anacardic Acid: molluscicide in cashew nut shell liquid: J.T. Sullivan, et al.; Planta Med. 44, 175 (1982) Abstract
Inhibition of lipoxygenase and prostaglandin endoperoxide synthase by anacardic acids: R. Grazzini, et al.; BBRC 176, 775 (1991) Abstract
Antibacterial activity of anacardic acid and totarol, alone and in combination with methicillin, against methicillin-resistant Staphylococcus aureus: H. Muroi & I. Kubo; J. Appl. Bacteriol. 80, 387 (1996) Abstract
Small molecule modulators of histone acetyltransferase p300: K. Balasubramanyam, et al.; J. Biol. Chem. 278, 19134 (2003) Abstract; Full Text
Inhibition of histone acetyltransferase activity by anacardic acid sensitizes tumor cells to ionizing radiation: Y. Sun, et al.; FEBS Lett. 580, 4353 (2006) Abstract
General Literature References
Antibacterial activity of anacardic acid and totarol, alone and in combination with methicillin, against methicillin-resistant Staphylococcus aureus: H. Muroi and I. Kubo; J. Appl. Bacteriol. 80, 387 (1996) Abstract
Synthesis of sildenafil analogues from anacardic acid and their phosphodiesterase-5 inhibition: R. Paramashivappa, et al.; J. Agric. Food Chem. 50, 7709 (2002) Abstract
Further Categories Containing This Product:
Lipoxygenases / Related Products • Prostaglandin Synthases / Related Products • Antibiotics Other Products
 
 
ALX-201-116 Revised 21-Jan-08
Annexin V (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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ALX-201-116-C100   100 µg 194.00 USD Add To Cart
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Product Specification
MW: ~52kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. Recombinant human annexin V fused to GST.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Affinity purified.
FORMULATION: Lyophilized. Contains PBS, pH 7.4, and 0.02% sodium azide.
RECONSTITUTION: Reconstitute with 100µl sterile water.
SPECIFICITY: Binds to phosphatidylserine (PS).
BIOLOGICAL ACTIVITY: Exhibits anti-phospholipase activity.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Reconstituted solutions can be kept at 4°C under sterile conditions for one week or should be frozen in aliquots at -20°C. Repeated freeze/thaw cycles (>3) may result in an adverse effect on the activity of annexin V.
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-209-257 Revised 18-May-06
Annexin V (mouse) (recombinant) (Alexa Fluor® 488)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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ALX-209-257-T100   100 tests 245.00 USD Add To Cart
Product Specification
QUANTITY: 100 tests/500µl.
FORMULATION: Liquid. Contains 0.02% sodium azide.
SPECIFICITY: Binds phosphatidylserine (PS) residues.
APPLICATION: Detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Protect from light. Do not freeze.
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Product Description
Alexa Fluor® 488-labelled recombinant mouse annexin shows bright green fluorescence (Ex(max): 495nm; Em(max): 519nm).
Product Specific Literature References
[1] Engagement of the antigen-receptor on immature murine B lymphocytes results in death by apoptosis: A. Norvell, et al.; J. Immunol. 154, 4404 (1995) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION General Protocol: Detection of apoptotic cells by flow cytometry

- Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS).
- Resuspend cells in 100µl binding buffer (10mM HEPES, pH 7.4, 140mM sodium chloride, 2.5mM calcium dichloride), add 5µl annexin V-Alexa Fluor® 488 to the cells and mix gently.
  For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1µg/ml or 1.5µM).
- Incubate for 15 min. at room temperature in the dark.
- Add 400µl binding buffer and analyze the staining in flow cytometry as soon as possible (within an hour).

Swiss-Prot link P48036: Annexin V (mouse)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-209-252 Revised 26-Aug-08
Annexin V (human) (recombinant) (APC)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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ALX-209-252-T020   20 tests 106.00 USD Add To Cart
ALX-209-252-T100   100 tests 279.00 USD Add To Cart
Product Specification
MW: ~35.8kDa.
SOURCE/HOST: Produced in E. coli.
QUANTITY: 20 tests/100µl; 100 tests/500µl.
PURITY: ≥98% (SDS-PAGE, HPLC)
FORMULATION: Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Do not freeze. Protect from light.
Product Description
APC-labelled recombinant human annexin V is suitable for dual staining experiments to detect phosphatidylserine whilst cell-surface protein can be detected with a suitable MAb (FITC-labelled or labelled secondary antibody). The fluorochrome APC (allophycocyanin) shows far red fluorescence (Ex(max): 650nm; Em(max): 660nm).
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:

 - Prepare binding buffer (10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2;
    filtered through 0.2µm pore filter);
 - Brief centrifugation before use is recommended
 - Wash cells in PBS by gentle shaking or pipetting up and down
 - resuspend cells in binding buffer; adjust cell density to 2-5x105/ml
 - Take 195µl cell suspension and add 5µl Annexin V-FITC
 - Mix and incubate 10 min. in the dark at room temperature
 - Wash cells 1x with PBS and resuspend in 190µl binding buffer
 - FACS analysis

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281 : Annexin V (mouse)
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-209-258 Revised 29-May-06
Annexin V (human) (recombinant) (ATTO 488)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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ALX-209-258-T100   100 tests 260.00 USD Add To Cart
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Product Specification
MW: ~52kDa (SDS-PAGE).
QUANTITY: 100 tests/0.5ml
PURITY: ≥90%
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry or immunofluorescence.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least one year after receipt when stored at +4°C.
HANDLING: Do not freeze. Protect from light.
Product Description
New ATTO-fluorescent dyes show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 488 shows bright green fluorescence (λabs (max): 501nm, λem (max): 523nm, εmax: 90'000).
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:

- Wash cells in PBS, and resuspend in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm filter).
- Adjust cells to a density between 200’000 and 500’000 cells/ml.
- Take 195µl cell suspension and add 5µl Annexin-ATTO 488. Mix and incubate 10 min. in the dark.
- Wash cells 1x and resuspend in 190µl binding buffer.
- Add 10µl of 20µg/ml propidium iodide (PI) stock solution to stain for necrotic cells.
- Analyze by FACS.
Note: These are general recommendations. Optimal conditions must be determined individually for each application:

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-209-259 Revised 11-Oct-06
Annexin V (human) (recombinant) (ATTO 647N)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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ALX-209-259-T100   100 tests 260.00 USD Add To Cart
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Product Specification
MW: ~52kDa (SDS-PAGE).
QUANTITY: 100 tests/0.5ml
PURITY: ≥90%
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry or immunofluorescence.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least one year after receipt when stored at +4°C.
HANDLING: Protect from light. Do not freeze.
Product Description
New ATTO-fluorescent dyes show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 647N shows red fluorescence (λabs (max): 645nm, λem (max): 669nm, εmax: 120’000).
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:

- Wash cells in PBS, and resuspend in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm filter).
- Adjust cells to a density between 200’000 and 500’000 cells/ml.
- Take 195µl cell suspension and add 5µl Annexin-ATTO 647N. Mix and incubate 10 min. in the dark.
- Wash cells 1x and resuspend in 190µl binding buffer.
- Add 10µl of 20µg/ml propidium iodide (PI) stock solution to stain for necrotic cells.
- Analyze by FACS.
Note: These are general recommendations. Optimal conditions must be determined individually for each application:

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-209-002 Revised 02-Aug-06
Annexin V (human) (recombinant) (Biotin)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-209-002-T020   20 tests 106.00 USD Add To Cart
ALX-209-002-T100   100 tests 352.00 USD Add To Cart
ALX-209-002-T300   300 tests 531.00 USD Add To Cart
Product Specification
MW: ~35.8kDa.
QUANTITY: 20 tests/100µl; 100 tests/500µl; 300 tests/1'500µl.
PURITY: ≥98% (SDS-PAGE, HPLC)
FORMULATION: Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
[1] Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers: H.A. Andree, et al.; J. Biol. Chem. 265, 4923 (1990) Abstract; Full Text
[2] Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages: V.A. Fadok, et al.; J. Immunol. 148, 2207 (1992) Abstract
[3] Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis: G. Koopman, et al.; Blood 84, 1415 (1994) Abstract
[4] Human neutrophils lose their surface Fc gamma RIII and acquire Annexin V binding sites during apoptosis in vitro: C.H. Homburg, et al.; Blood 85, 532 (1995) Abstract
[5] A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V: I. Vermes, et al.; J. Immunol. Meth. 184, 39 (1995) Abstract
[6] Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl: S. Martin, et al.; J. Exp. Med. 182, 1545 (1995) Abstract
[7] Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry 24, 123 (1996) Abstract
[7] Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry 24, 123 (1996) Abstract
[8] CD95 engagement releases calcium from intracellular stores of long term activated, apoptosis-prone gammadelta T cells: P. Rovere, et al.; J. Immunol. 156, 4631 (1996) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:
- Brief centrifugation before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down
- Resuspend cells in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered
through 0.2µm pore filter); adjust cell density to 2-5x105/ml
- Take 195µl cell suspension and add 5µl Annexin V-Biotin
- Mix and incubate 15 minutes at room temperature
- Wash cells 2x in binding buffer
- Resuspend in binding buffer
- Add Streptavidin-FITC
- Mix and incubate for 30 minutes in the dark at room temperature
- Wash cells 1x in binding buffer and resuspend in 190µl binding buffer
- Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1mg/ml)
- FACS analysis

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281 : Annexin V (mouse)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • Biotinylated Reagents Other Products
 
 
ALX-209-250 Revised 08-Aug-08
Annexin V (human) (recombinant) (FITC)