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Granzymes
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ALX-201-118 Revised 23-May-07
Granzyme A (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Granzymes
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-118-C010   10 µg 310.00 USD Add To Cart
Product Specification
MW: ~52kDa (homodimer).
EC: 3.4.21.78
SOURCE/HOST: Produced in E. coli. Active human granzyme A.
CONCENTRATION: 0.2mg/ml.
FORMULATION: Liquid. In 0.05M MES, pH 6.0, containing 10% glycerol and ~0.52M sodium chloride. Contains no preservatives.
SPECIFIC ACTIVITY: ~110U/µg protein. One unit is defined as the amount of enzyme that hydrolyzes 1nmol Z-Lys-SBzl per min. at 25°C in 0.05M TRIS, pH 8.0, containing 0.15M NaCl, 0.01% Triton X-100 and 0.3mM DTNB.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -80°C. Do not dilute to more than 0.2mg/ml. Do not store dilute solutions (1-1000ng/ml) for more than 24 hours.
HANDLING: Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After opening, prepare aliquots and store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P12544: Granzyme A (human) (precursor)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-200-605 Revised 23-Apr-05
Granzyme A (human)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Granzymes
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-200-605-C010   10 µg 460.00 USD Add To Cart
Product Specification
MW: ~52kDa (fully glycosylated homodimer)
EC: 3.4.21.78
SOURCE/HOST: Human cytotoxic granules of NK-92 cells.
CONCENTRATION: 2.76mg/ml
PURITY DETAIL: Purified by differential solubilization and cation exchange chromatography. Dialyzed against 0.15M sodium chloride and concentrated.
FORMULATION: Liquid. In ~0.15M sodium chloride.
SPECIFIC ACTIVITY: ~9.2U/µg protein. One unit is defined as the amount of enzyme that hydrolyzes 1nmole of Z-Lys-SBzl per min. at 25°C in 0.05M TRIS, pH 8.0, containing 0.15M NaCl, 0.01% Triton X-100, 0.3mM DTNB.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 6 months when stored at -80°C. Do not store dilute solutions (1-1000ng/ml) for more than 24 hours.
HANDLING: Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
Granzyme A induces caspase-independent mitochondrial damage, a required first step for apoptosis: D. Martinvalet, et al.; Immunity 22, 355 (2005) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P12544: Granzyme A (human) (precursor)
Further Categories Containing This Product:
Enzymes • Natural Proteins
 
 
ALX-200-602 Revised 16-Oct-07
Granzyme B (human)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Granzymes
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-200-602-C010   10 µg 495.00 USD Add To Cart
Product Specification
MW: ~32kDa.
EC: 3.4.21.79
SOURCE/HOST: Isolated from IL-2 activated human lymphocytes [1]. Active enzyme.
CONCENTRATION: ~5.6mg/ml
FORMULATION: Liquid. In 150mM sodium chloride. Contains no preservatives.
BIOLOGICAL ACTIVITY: Using standard cell lines, 10µg is sufficient to kill 1x108 cells [2].
SPECIFIC ACTIVITY: ≥10U/µg protein. One unit is defined as the amount of enzyme that hydrolyzes 1nmol BAADT per min. (Prod. No. ALX-260-050) [1].
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Do not dilute below 1mg/ml. Dilute solutions should be used within 24 hours.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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Product Specific Literature References
[1] Rapid purification of cationic granule proteases: application to human granzymes: W.L. Hanna, et al.; Protein Purif. Exp. 4, 398 (1993) Abstract
[2] New paradigm for lymphocyte granule-mediated cytotoxicity. Target cells bind and internalize granzyme B, but an endosomolytic agent is necessary for cytosolic delivery and subsequent apoptosis: C.J. Froelich, et al.; J. Biol. Chem. 271, 29073 (1996) Abstract; Full Text
[3] Granzyme B binds to target cells mostly by charge and must be added at the same time as perforin to trigger apoptosis: L. Shi, et al.; J. Immunol. 174, 5456 (2005) Abstract
[4] Granzyme A induces a pro-inflammatory cytokine response and not cell death.: C. J. Froelich, et al.; Immunity Epub ahead of print, (2007)
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P10144: Granzyme B (human) (precursor)
Further Categories Containing This Product:
Natural Proteins • Enzymes
 
 
ALX-201-112 Revised 18-Mar-08
Granzyme B (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Granzymes
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-112-C010   10 µg 310.00 USD Add To Cart
Product Specification
MW: ~25.5kDa.
EC: 3.4.21.79
SOURCE/HOST: Produced in E. coli. Active human granzyme B.
CONCENTRATION: 0.2mg/ml
FORMULATION: Liquid. In PBS containing 10% glycerol. Contains no preservatives.
SPECIFIC ACTIVITY: ≥7.0U/µg protein. One unit is defined as the amount of enzyme that hydrolyzes 1nmol Ac-IEPD-pNA (Prod. No. ALX-260-152) per min. at 25°C in 0.05M TRIS, pH 8.0, containing 0.15M NaCl and 0.01% Triton X-100.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Dilute solutions (e. g. 1-1000ng/ml) should be used within 24 hours.
HANDLING: For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
Crystal structure of the caspase activator human granzyme B, a proteinase highly specific for an Asp-P1 residue: E. Estebanez-Perpina, et al.; Biol. Chem. 381, 1203 (2000) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P10144: Granzyme B (human) (precursor)
General Literature References
Definition and redesign of the extended substrate specificity of granzyme B  : J.L. Harris, et al.; J. Biol. Chem. 273, 27364 (1998) Abstract; Full Text
Generation of catalytically active granzyme K from Escherichia coli inclusion bodies and identification of efficient granzyme K inhibitors in human plasma  : E. Wilharm, et al.; J. Biol. Chem. 274, 27331 (1999) Abstract; Full Text
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-117 Revised 13-Dec-04
Granzyme K (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Granzymes
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-117-C010   10 µg 310.00 USD Add To Cart
Product Specification
MW: ~26kDa.
EC: 3.4.21.-
SOURCE/HOST: Produced in E. coli. Active human granzyme K.
CONCENTRATION: 0.2mg/ml
FORMULATION: Liquid. Recombinant protein in PBS containing glycerol. Does not contain any preservatives.
SPECIFIC ACTIVITY: ~29U/µg protein. One unit is defined as the amount of enzyme that hydrolyzes 1nmol Z-Lys-SBzl per min. at 25°C in 0.05M TRIS, pH 8.0, containing 0.15M NaCl, 0.01% Triton X-100 and 0.3mM DTNB.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 6 months when stored at -80°C. Dilute solutions (e. g. 1-1000ng/ml) should be used within 24 hours.
HANDLING: For maximum product recovery after thawing, centrifuge the vial before opening the cap. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
Generation of catalytically active granzyme K from Escherichia coli inclusion bodies and identification of efficient granzyme K inhibitors in human plasma: E. Wilharm, et al.; J. Biol. Chem. 274, 27331 (1999) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P49863: Granzyme K (human) (precursor)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
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