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ALX-201-042
Revised 09-Mar-06
MMP-3 (Catalytic Domain) (human) (recombinant)
SYNONYMS
Stromelysin-1 (human) (recombinant)
Matrix Metalloproteinase 3 (Catalytic Domain) (human) (recombinant)
Transin-1 (human) (recombinant)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
MMPs
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-042-C005
5 µg
197.00 USD
Product Specification
MW:
~22kDa.
EC:
3.4.24.17
SOURCE/HOST:
Produced in
E. coli
.
CONCENTRATION:
PURITY DETAIL:
No other MMP contaminants are detectable.
FORMULATION:
Liquid. In 50mM Tris-HCl, pH 7.5, containing 10mM CaCl
2
, 1µM ZnCl
2
and 0.05% sodium azide.
SPECIFIC ACTIVITY:
≥900mU/mg protein (H. Nagase, et al; J. Biol. Chem.
269
, 20952 (1994)). One unit is defined as the amount of enzyme that hydrolyzes 1µmol Mca-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(DNP)-NH
2
per min. at 37°C, pH 7.0.
SHIPPING:
SHIPPED ON DRY ICE
SHORT TERM STORAGE:
-20°C
LONG TERM STORAGE:
-80°C
USE/STABILITY:
Stable for several weeks when stored at at -20°C and for at least 1 week when stored at at +4°C.
HANDLING:
Avoid freeze/thaw cycles.
Product Description
Highly purified recombinant enzyme containing the 22kDa catalytic domain of MMP-3.
Product Specific Literature References
Stromelysin is an activator of procollagenase. A study with natural and recombinant enzymes:
G. Murphy, et al.; Biochem. J.
248
, 265 (1987)
Abstract
Evidence that human rheumatoid synovial matrix metalloproteinase 3 is an endogenous activator of procollagenase:
A. Ito & H. Nagase; Arch. Biochem. Biophys.
267
, 211 (1988)
Abstract
Matrix metalloproteinase 3 (stromelysin) activates the precursor for the human matrix metalloproteinase 9:
Y. Ogata, et al.; J. Biol. Chem.
267
, 3581 (1992)
Abstract
;
Full Text
A trypsin sensitive stromelysin isolated from rheumatoid synovial fluid is an activator for matrix metalloproteinases:
H. Kolkenbrock, et al.; Eur. J. Clin. Chem. Clin. Biochem.
31
, 625 (1993)
Abstract
Matrix metalloproteinase-3 (stromelysin-1). Identification as the cartilage acid metalloprotease and effect of pH on catalytic properties and calcium affinity:
S.M. Wilhelm, et al.; J. Biol. Chem.
268
, 21906 (1993)
Abstract
;
Full Text
Design and characterization of a fluorogenic substrate selectively hydrolyzed by stromelysin 1 (matrix metalloproteinase-3):
H. Nagase, et al.; J. Biol. Chem.
269
, 20952 (1994)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
MMP-3 hydrolyzes components of the extracellular matrix like proteoglycan, laminin, fibronectin, gelatin and collagen types III, IV and IX. It also activates pro-MMP-9 and pro-MMP-8 and superactivates plasmin activated MMP-1. MMP-3 is secreted as a latent proenzyme and is activated by a variety of proteinases, e.g. plasmin, trypsin, chymotrypsin, cathepsin G or human neutrophil elastase. The activating process generates an active 45kDa species which converts easily to a highly active 28kDa form. Product ALX-201-042 displays the same activity as the 28kDa form.
Swiss-Prot link P08254:
MMP-3 (human) (precursor)
AfCS Signalling Gateway link A001476:
MMP-3 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-047
Revised 14-Jul-08
Caspase-9 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-047-U025
25 U
231.00 USD
ALX-201-047-U100
100 U
399.00 USD
Product Specification
EC:
3.4.22.62
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with 25µl or 100µl PBS containing 15% glycerol.
Note:
It is normal to see some insoluble protein following reconstitution. This is mainly due to denaturation of small amounts of protein during preparation. Precipitate should not affect activity. If preferred, remove precipitate by brief centrifugation.
SPECIFIC ACTIVITY:
~400U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate LEHD-
p
NA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in screening caspase inhibitors, studying enzyme regulation, determining target substrates or as positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Specific Literature References
The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32:
S.M. Srinivasula, et al.; J. Biol. Chem.
271
, 27099 (1996)
Abstract
;
Full Text
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade:
P. Li, et al.; Cell
91
, 479 (1997)
Abstract
A conserved XIAP-interaction motif in caspase-9 and Smac/DIABLO regulates caspase activity and apoptosis:
S.M. Srinivasula, et al.; Nature
410
, 112 (2001)
Abstract
ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B:
H. Duan, et al.; J. Biol. Chem.
271
, 16720 (1996)
Abstract
;
Full Text
Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex:
G. Pan, et al.; J. Biol. Chem.
273
, 5841 (1998)
Abstract
;
Full Text
General Information
Caspase-9 (also known as Ice-Lap6 and Mch6) is a member of the interleukin-1
b converting enzyme (ICE) family of cysteine proteases. Similar as other caspases, caspase-9 also exists in cells as an inactive proenzyme. During the initiation of apoptosis procaspase-9 is processed at aspartate residues to form active caspase-9. As one of the initiator caspases, active caspase-9 functions to trigger the activation of downstream effector caspases, leading to disassembly of cell structures. The active recombinant caspase-9 is routinely tested for its ability to enzymatically cleave the caspase-9 colorimetric substrate Ac-LEHD-pNA (Prod. No.
ALX-260-081
).
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P55211:
Caspase-9 (human) (precursor)
AfCS Signalling Gateway link A000504:
Caspase-9 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-055
Revised 17-Apr-07
Phosphoinositide 3-kinase p110γ (human) (recombinant)
SYNONYMS
PI(3)K p110γ (human) (recombinant)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Phosphoinositide 3-kinase [PI(3)K] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-055-C010
10 µg
330.00 USD
Product Specification
EC:
2.7.1.137
SOURCE/HOST:
Produced in Sf9 cells. Full length PI(3)K p110γ (human phosphoinositide-3-kinase p110γ) is fused at the N-terminus to a His-tag.
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In 10mM HEPES, pH 7.5, containing 100mM sodium chloride, 0.5mM magnesium chloride and 50% glycerol.
SPECIFIC ACTIVITY:
~4.5nmol/mg/min using phosphatidylinositol as the substrate (lipid kinase activity test).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-20°C
Product Specific Literature References
Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase:
B. Stoyanov, et al.; Science
269
, 690 (1995)
Abstract
Linkage of G protein-coupled receptors to the MAPK signaling pathway through PI 3-kinase gamma:
M. Lopez-Ilasaca, et al.; Science
275
, 394 (1997)
Abstract
Lipid kinase and protein kinase activities of G-protein-coupled phosphoinositide 3-kinase gamma: structure-activity analysis and interactions with wortmannin:
S. Stoyanova, et al.; Biochem. J.
324
, 489 (1997)
Abstract
;
Full Text
Gbetagamma stimulates phosphoinositide 3-kinase-gamma by direct interaction with two domains of the catalytic p110 subuni:
D. Leopoldt, et al.; J. Biol. Chem.
273
, 7024 (1998)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P48736:
PI(3)K p110γ (human)
Lipid kinase activity test
:
Kinase reaction buffer: 20mM TRIS-HCl, pH 7.4; 4mM MgCl
2
; 100mM sodium chloride.
- Reaction mixture in total volume of 100ml was:
1x reaction buffer (final concentration), enzyme (4 reactions with 1, 2, 3 or 4µg were performed), 10µg phosphatidylinositol, 20µM cold ATP and 10µCi
32
P-ATP.
- Reaction was started by addition of the
32
P-ATP and incubate for 10 minutes at 37°C.
- Reaction was stopped by adding 150µl 1M HCl.
- L
ipids were extracted with 400µl chloroform:methanol (1:1) by vortexing.
- Extracts were washed
three times with 200µl 1M HCl.
- Incorporation of
32
P in the extracted lipids was measured by mixing extract with 3ml scintillation cocktail (ROTH) and counting in a liquid scintillation analyzer.
- To determine specific activity of PI3K p110γ the assay was performed under the same conditions stated above by using 10µCi
32
P-ATP with a value of 10'000 units.
- Average value of measurements of the lipid extracts of the performed 4 reactions was 227 units for 1µg.
Dividing 10’000 by 227 a factor of 44 times less for the incorporation was determined.
-Total ATP (20µM) was divided by 44 giving 0.454µM for 10 minutes reaction time or 45.4M/min/µg. Recalculation of this value for a 1 liter solution results in
4.5 nmoles/min/mg
.
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-056
Revised 21-Jul-08
Caspase-1 (active) (human) (recombinant)
SYNONYMS
Interleukin 1
β Converting Enzyme (active) (human) (recombinant)
IL-1β Converting Enzyme (active) (human) (recombinant)
ICE (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-056-U025
25 U
231.00 USD
ALX-201-056-U100
100 U
385.00 USD
Product Specification
EC:
3.4.22.36
SOURCE/HOST:
Produced in
E. coli
.
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
~20’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate YVAD-
p
NA (
p
NA:
p
-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
QUALITY CONTROL:
Routinely tested for its ability to cleave the caspase-1 colorimetric substrate Ac-YVAD-
p
NA (Prod. No.
ALX-260-026
).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
Caspase-1 is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20kDa) and small (10kDa) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form.
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P29466:
Caspase-1 (human) (precursor)
AfCS Signalling Gateway link A000492:
Caspase-1 (mouse)
Further Categories Containing This Product:
Inflammasome
•
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-057
Revised 30-Jun-08
Caspase-2 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-057-U025
25 U
203.00 USD
ALX-201-057-U100
100 U
371.00 USD
Product Specification
EC:
3.4.22.36
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥
95% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
10'000-13'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate VDVAD-pNA per hour at 37°C in reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in screening capase inhibitors, studying enzyme regulation and kinetics, determining specificity of capase substrates, or as positive control in caspase activity assays.
We recommend using 1 unit per assay for analyzing caspase activity.
For a complete caspase-2 assay protocol, please refer to Caspase-2 Fluorometric or Colorimetric Assay Kits (Prod. No.
ALX-850-214
or
ALX-850-213
).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
General Information
Caspase-2 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. Similar as other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis, procaspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed form of caspase-2 consists of large (19kDa) and small (12kDa) subunits which associate to form the active enzyme. The expressed caspase-2 spontaneously undergoes auto-processing to yield the subunits characteristic of the native enzyme. The active recombinant caspase-2 is routinely tested for its ability to enzymatically cleave the caspase-2 colorimetric substrate Ac-VDVAD-pNA (Prod. No.
ALX-260-059
). The signal can be detected by spectrophotometry.
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P42575:
Caspase-2 (human) (precursor)
AfCS Siganlling Gateway link A000497:
Caspase-2 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-059
Revised 21-Jul-08
Caspase-3 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-059-U025
25 U
119.00 USD
ALX-201-059-U100
100 U
273.00 USD
Product Specification
EC:
3.4.22.56
SOURCE/HOST:
Produced in
E. coli
.
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
300’000-400’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD-
p
NA (Prod. No.
ALX-260-033
) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in studying enzyme regulation, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
For a complete caspase-3 assay protocol, please refer to Caspase-3 Fluorometric or Colorimetric Assay Kits (Prod. No.
ALX-850-216
or
ALX-850-215
).
QUALITY CONTROL:
Routinely tested for its ability to cleave the caspase-3 substrates Ac-DEVD-
p
NA (Prod. No.
ALX-260-033
) and Ac-DEVD-AFC (Prod. No.
ALX-260-032
).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
General Information
Caspase-3 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. It exists in cells as an inactive 32kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6, caspase-8 and granzyme B. The processed form of caspase-3 consists of large (17kDa) and small (11kDa) subunits which associate to form the active enzyme. Active caspase-3 has been shown involved in the proteolysis of several important molecules, such as poly(ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK) and others.
BACKGROUND/TECHNICAL INFORMATION
Swiss Prot link:
Caspase-3 (human) (precursor)
AfCS Signalling Gateway link:
Caspase-3 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-060
Revised 02-Jun-08
Caspase-6 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-060-U025
25 U
203.00 USD
ALX-201-060-U100
100 U
371.00 USD
Product Specification
EC:
3.4.22.59
SOURCE/HOST:
Produced in
E. coli.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
BIOLOGICAL ACTIVITY:
Cleaves substrates exhibiting consensus sequence VEID, e.g. Ac-VEID-AFC (Prod. No.
ALX-260-111
), Ac-VEID-AMC (Prod. No.
ALX-260-064
) and Ac-VEID-pNA (Prod. No.
ALX-260-063
).
SPECIFIC ACTIVITY:
~13'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate VEID-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1U per assay for analyzing caspase activity.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
General Information
Caspase-6 (also known as Mch2) is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. Same as other caspases, caspase-6 also exists in cells as an inactive proenzyme. During apoptosis procaspase-6 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed form of caspase-6 consists of large (18kDa) and small (11kDa) subunits which associate to form the active enzyme. The active caspase-6 has been shown involving in the proteolysis of poly(ADP-ribose) polymerase (PARP), an enzyme that is involved in DNA repair and genomic maintenance.
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P55212:
Caspase-6 (human) (precursor)
AfCS Signalling Gateway link A000501:
Caspase-6 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-061
Revised 21-Jul-08
Caspase-7 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-061-U025
25 U
203.00 USD
ALX-201-061-U100
100 U
371.00 USD
Product Specification
EC:
3.4.22.60
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
~25’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD-
p
NA
(p
NA:
p
-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P55210:
Caspase-7 (human) (precursor)
AfCS Signalling Gateway link A000502:
Caspase-7 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-062
Revised 04-Aug-08
Caspase-8 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-062-U025
25 U
203.00 USD
ALX-201-062-U100
100 U
371.00 USD
Product Specification
EC:
3.4.22.-
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
5’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate IETD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1U per assay for analyzing caspase activity.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
General Information
Caspase-8 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. Same as other caspases, caspase-7 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed form of caspase-8 consists of large and small subunits which associate to form the active enzyme.
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link Q14790:
Caspase-8 (human) (precursor)
AfCS Signalling Gateway link A000503:
Caspase-8 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
