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Acute Phase Response Proteins / Related Products
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ALX-201-124 Revised 20-Jun-08
PTX3 (human) (recombinant)
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SYNONYMS Pentraxin 3 (human) (recombinant)
TSG14 (human) (recombinant)
TNF Stimulated Gene-14 (human) (recombinant)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Pentraxin3 [PTX3] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-124-C010   10 µg 298.00 USD Add To Cart
Product Specification
MW: ~42kDa. Due to glycosylation, PTX3 may migrate between 40-45kDa.
SOURCE/HOST: Produced in CHO cells. Human full-length PTX3.
CONCENTRATION: 0.1mg/ml.
FORMULATION: Liquid. In PBS containing 2% BSA and 0.1% sodium azide.
APPLICATION: ELISA and Western blot standard together with MAb to PTX3 (Prod. No. ALX-804-464 (capture antibody)) and PAb to PTX3-Biotin (Prod. No. ALX-210-365 (detection antibody)).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Multimer Formation and Ligand Recognition by the Long Pentraxin PTX3. Similarities and differences with the short pentraxins C-reactive protein and serum amyloid P component: B. Bottazzi, et al.; J. Biol. Chem. 272, 32817 (1997) Abstract; Full Text
Expression of a long pentraxin, PTX3, by monocytes exposed to the mycobacterial cell wall component lipoarabinomannan: V. Vouret-Craviari, et al.; Infect. and Immunity 65, 1345 (1997) Abstract
PTX3, A prototypical long pentraxin, is an early indicator of acute myocardial infarction in humans: G. Peri, et al.; Circulation 102, 636 (2000) Abstract; Full Text
The long pentraxin PTX3 binds to apoptotic cells and regulates their clearance by antigen-presenting dendritic cells: P. Rovere, et al.; Blood 96, 4300 (2000) Abstract; Full Text
PTX3 in small-vessel vasculitides: an independent indicator of disease activity produced at sites of inflammation: F. Fazzini, et al.; Arthritis Rheum. 44, 2841 (2001) Abstract
Dynamic induction of the long pentraxin PTX3 in the CNS after limbic seizures: evidence for a protective role in seizure-induced neurodegeneration: T. Ravizza, et al.; Neuroscience 105, 43 (2001) Abstract
Circulating levels of the long pentraxin PTX3 correlate with severity of infection in critically ill patients: B. Muller, et al.; Crit. Care Med. 29, 1404 (2001) Abstract
Non-redundant role of the long pentraxin PTX3 in anti-fungal innate immune response: C. Garlanda, et al.; Nature 420, 182 (2002) Abstract
Long pentraxin PTX3 upregulates tissue factor expression in human endothelial cells: a novel link between vascular inflammation and clotting activation: E. Napoleone, et al.; Art. Thromb. Vasc. Biol. 22, 782 (2002) Abstract
The long pentraxin Ptx3 is synthesized in IgA glomerulonephritis and activates mesangial cells: B. Bussolati, et al.; J. Immunol. 170, 1466 (2003) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Sandwich ELISA for PTX3

- 96 well-ELISA  plates (Nunc MaxiSorp 446612) were coated with 100µl of MAb to PTX3 (human) (MNB4) (Prod. No. ALX-804-464), 700ng/ml in 100µl coating buffer (15mM carbonate buffer pH 9.6) and incubated overnight at 4°C.
- After incubation, the plates were washed three times with 300µl/well of washing buffer (PBS + 0.05% Tween 20), and then 300µl of 5% dry milk in washing buffer were added to block non-specific binding.
- The plates were incubated for 2 hours at room temperature and then washed three times with washing buffer.
- 50µl (in duplicate) recombinant human PTX3 standard (Prod. No. ALX-201-124) (75pg/ml to 2.4ng/ml ), cell supernatants or biological fluids diluted in RPMI 1640 + 2% BSA were added and then incubated for 2 hours at 37°C.
- The plates were washed five times with washing buffer, and then 100µl of PAb to PTX3 (human) (Biotin) (Prod. No. ALX-210-365B) 25ng/ml in washing buffer were added in each well.
- The plates were incubated for 1 hour at 37°C and then washed five times with 300µl of washing buffer.
- 100µl/well streptavidin-horseradish peroxidase diluted 1:4’000 were added and the plates incubated for 1 hour at room temperature.
- After incubation the plates were washed 5 times and 100µl of chromogen substrate ABTS were added. Plates were read after 15 minutes at 405nm in an automatic ELISA reader.
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-804-463 Revised 31-Oct-06
Monoclonal Antibody to PTX3 (MNB1)
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SYNONYMS anti-Pentraxin 3 MAb (MNB1)
anti-TSG14 MAb (MNB1)
anti-TNF Stimulated Gene-14 MAb (MNB1)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Pentraxin3 [PTX3] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-463-C100   100 µg 350.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: MNB1
ISOTYPE: Rat IgG2b
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Purified antibody in PBS containing 0.1% sodium azide.
IMMUNOGEN: Recombinant human PTX3 (pentraxin 3).
SPECIFICITY: Recognizes the C-terminus of human and mouse PTX3.
APPLICATION: Immunohistochemistry (frozen sections, paraffin sections)
Western Blot (excellent)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-464 Revised 06-Nov-07
Monoclonal Antibody to PTX3 (MNB4)
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SYNONYMS anti-Pentraxin 3 MAb (MNB4)
anti-TSG14 MAb (MNB4)
anti-TNF Stimulated Gene-14 MAb (MNB4)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Pentraxin3 [PTX3] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-464-C100   100 µg 350.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
CLONE: MNB4
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 0.1% sodium azide.
IMMUNOGEN: Recombinant human PTX3 (pentraxin 3).
SPECIFICITY: Recognizes the N-terminus of human and rat PTX3.
APPLICATION: ELISA (capture - excellent)
Immunohistochemistry (frozen sections (1µg/ml))
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
Multimer formation and ligand recognition by the long pentraxin PTX3. Similarities and differences with the short pentraxins C-reactive protein and serum amyloid P component: B. Bottazzi, et al.; J. Biol. Chem. 272, 32817 (1997) Abstract
PTX3, A Prototypical Long Pentraxin, is an Early Indicator of Acute Myocardial Infarction in Humans: G. Peri, et al.; Circulation 102, 636 (2000) Abstract; Full Text
Production of the Long Pentraxin PTX3 in Advanced Atherosclerotic Plaques: M.S. Rolph, et al.; Arterioscler. Thromb. Vasc. Biol. 22, e10 (2002) Abstract
The long Pentraxin Ptx3 is synthesized in IgA glomerulonephritis and Activates Mesangial Cells: B. Bussolati, et al.; J. Immunol. 170, 1466 (2003) Abstract
Pentraxin 3 is elevated in haemodialysis patients and is associated with cardiovascular disease: M. Boehme, et al.; Nephrol. Dial. Transplant. 22, 2224 (2007) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Sandwich ELISA for PTX3

- 96 well-ELISA  plates (Nunc MaxiSorp 446612) were coated with 100µl of MAb to PTX3 (human) (MNB4) (Prod. No. ALX-804-464), 700ng/ml in 100µl coating buffer (15mM carbonate buffer pH 9.6) and incubated overnight at 4°C.
- After incubation, the plates were washed three times with 300µl/well of washing buffer (PBS + 0.05% Tween 20), and then 300µl of 5% dry milk in washing buffer were added to block non-specific binding.
- The plates were incubated for 2 hours at room temperature and then washed three times with washing buffer.
- 50µl (in duplicate) recombinant human PTX3 standard (Prod. No. ALX-201-124) (75pg/ml to 2.4ng/ml ), cell supernatants or biological fluids diluted in RPMI 1640 + 2% BSA were added and then incubated for 2 hours at 37°C.
- The plates were washed 5 x with washing buffer, and then 100µl of PAb to PTX3 (human) (Biotin) (Prod. No. ALX-210-365) 25ng/ml in washing buffer were added to each well.
- The plates were incubated for 1 hour at 37°C and then washed five times with 300µl of washing buffer.
- 100µl/well streptavidin-horseradish peroxidase diluted 1:4’000 were added and the plates incubated for 1 hour at room temperature.
- After incubation the plates were washed 5 x and 100µl of chromogen substrate ABTS were added. Plates were read after 15 minutes at 405nm in an automatic ELISA reader.
Related Products
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-210-365 Revised 06-Nov-07
Polyclonal Antibody to PTX3
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SYNONYMS anti-Pentraxin 3 PAb
anti-TSG14 PAb
anti-TNF Stimulated Gene-14 PAb
PRODUCT LINE Inflammation
PRODUCT CATEGORY Pentraxin3 [PTX3] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-365-C050   50 µg 370.00 USD Add To Cart
ALX-210-365B-C050 Biotin 50 µg 470.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In PBS containing 0.1% sodium azide.
IMMUNOGEN: Recombinant human PTX3.
SPECIFICITY: Recognizes human, mouse, rat  and bovine (weak) PTX3 and chicken homolog PPTX.
APPLICATION: ELISA (detection - excellent): 1:20’000. Use as detection antibody in an ELISA format together with MAb to PTX3 (Prod. No. ALX-804-464 (capture)) and PTX3 standard (Prod. No. ALX-201-124).
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Once thawed store biotinylated antibody at 4°C and do not re-freeze.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Inducible expression of PTX3, a new member of the pentraxin family, in human mononuclear phagocytes: V.V. Alles, et al.; Blood 84, 3483 (1994) Abstract; Full Text
Expression of a long pentraxin, PTX3, by monocytes exposed to the mycobacterial cell wall component lipoarabinomannan: V. Vouret-Craviari; Infect. and Immunity 65, 1345 (1997) Abstract
PTX3, A prototypical long pentraxin, is an early indicator of acute myocardial infarction in humans: G. Peri, et al.; Circulation 102, 636 (2000) Abstract; Full Text
The long pentraxin Ptx3 is synthesized in IgA glomerulonephritis and activates mesangial cells: B. Bussolati, et al.; J. Immunol. 170, 1466 (2003) Abstract
A secretory-type protein, containing a pentraxin domain, interacts with an A-type K+ channel: D.E. Duzhyy, et al.; J. Biol. Chem. 280, 15165 (2005) Abstract
Pentraxin 3 is elevated in haemodialysis patients and is associated with cardiovascular disease: M. Boehme, et al.; Nephrol. Dial. Transplant. 22, 2224 (2007) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Sandwich ELISA for PTX3

- 96 well-ELISA  plates (Nunc MaxiSorp 446612) were coated with 100µl of MAb to PTX3 (human) (MNB4) (Prod. No. ALX-804-464), 700ng/ml in 100µl coating buffer (15mM carbonate buffer pH 9.6) and incubated overnight at 4°C.
- After incubation, the plates were washed three times with 300µl/well of washing buffer (PBS + 0.05% Tween 20), and then 300µl of 5% dry milk in washing buffer were added to block non-specific binding.
- The plates were incubated for 2 hours at room temperature and then washed three times with washing buffer.
- 50µl (in duplicate) recombinant human PTX3 standard (Prod. No. ALX-201-124) (75pg/ml to 2.4ng/ml ), cell supernatants or biological fluids diluted in RPMI 1640 + 2% BSA were added and then incubated for 2 hours at 37°C.
- The plates were washed five times with washing buffer, and then 100µl of PAb to PTX3 (human) (Biotin) (Prod. No. ALX-210-365B) 25ng/ml in washing buffer were added in each well.
- The plates were incubated for 1 hour at 37°C and then washed five times with 300µl of washing buffer.
- 100µl/well streptavidin-horseradish peroxidase diluted 1:4'000 were added and the plates incubated for 1 hour at room temperature.
- After incubation the plates were washed 5 times and 100µl of chromogen substrate ABTS were added. Plates were read after 15 minutes at 405nm in an automatic ELISA reader.
Related Products
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-850-299 Revised 18-Aug-08
PTX3 (human) Detection Set [For ELISA Application]
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PRODUCT LINE Inflammation
PRODUCT CATEGORY Pentraxin3 [PTX3] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-299-KI01   1 Set 995.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: Detection limit: 75pg/ml.
KIT/SET CONTAINS: Contains
- 1 vial (10µg) PTX3 (human) (rec.) (Prod. No. ALX-201-124)
- 1 vial (50µg) PAb to PTX3 (Biotin) [Detection Antibody] (Prod. No. ALX-210-365B)
- 1 vial (100µg) MAb to PTX3 (human) (MNB4) [Capture Antibody]  (Prod. No. ALX-804-464)
APPLICATION: For the quantitative determination of PTX3 in human and bovine (weak) cell culture media, EDTA-plasma or serum (weak) samples.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Once thawed store biotinylated antibody at 4°C and do not re-freeze.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
DNA microarray analysis of gene expression in alveolar epithelial cells in response to TNFalpha, LPS, and cyclic stretch: C.C. dos Santos, et al.; Physiol. Genomics 19, 331 (2004) Abstract; Full Text
TNFalpha-induced long pentraxin PTX3 expression in human lung epithelial cells via JNK: B. Han, et al.; J. Immunol. 175, 8303 (2005) Abstract; Full Text
The tissue pentraxin PTX3 limits C1q-mediated complement activation and phagocytosis of apoptotic cells by dendritic cells: P. Baruah, et al.; J. Leukoc. Biol. 80, 87 (2006) Abstract; Full Text
Pentraxin 3 is elevated in haemodialysis patients and is associated with cardiovascular disease: M. Boehme, et al.; Nephrol. Dial. Transplant. 22, 2224 (2007) Abstract
Establishment of a high sensitivity plasma assay for human pentraxin3 as a marker for unstable angina pectoris: K. Inoue, et al.; Arterioscler. Thromb. Vasc. Biol. 27, 161 (2007) Abstract; Full Text
Calorie restriction modulates inactivity-induced changes in the inflammatory markers C-reactive protein and pentraxin-3: A. Bosutti, et al.; J. Clin. Endocrinol. Metab. 93, 3226 (2008) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Sandwich ELISA for PTX3

- 96 well-ELISA  plates (Nunc MaxiSorp 446612) were coated with 100µl of MAb to PTX3 (human) (MNB4) (Prod. No. ALX-804-464), 700ng/ml in 100µl coating buffer (15mM carbonate buffer pH 9.6) and incubated overnight at 4°C.
- After incubation, the plates were washed three times with 300µl/well of washing buffer (PBS + 0.05% Tween 20), and then 300µl of 5% dry milk in washing buffer were added to block non-specific binding.
- The plates were incubated for 2 hours at room temperature and then washed three times with washing buffer.
- 50µl (in duplicate) recombinant human PTX3 standard (Prod. No. ALX-201-124) (75pg/ml to 2.4ng/ml ), cell supernatants or biological fluids diluted in RPMI 1640 + 2% BSA were added and then incubated for 2 hours at 37°C.
- The plates were washed five times with washing buffer, and then 100µl of PAb to PTX3 (human) (Biotin) (Prod. No. ALX-210-365) 25ng/ml in washing buffer were added in each well.
- The plates were incubated for 1 hour at 37°C and then washed five times with 300µl of washing buffer.
- 100 µl/well streptavidin-horseradish peroxidase diluted 1:4’000 were added and the plates incubated for 1 hour at room temperature.
- After incubation the plates were washed 5 times and 100µl of chromogen substrate ABTS were added. Plates were read after 15 min. at 405nm in an automatic ELISA reader.

Note: Do not use heparin plasma or serum samples with this assay. EDTA samples are recommended. The detection value in serum is generally less than in plasma samples.
Further Categories Containing This Product:
ELISA Sets
 
 
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