ALEXIS Biochemicals: Polyclonal Antibody to Toll-like Receptor 4

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ALX-804-130

Revised 31-Oct-06

Monoclonal Antibody to Toll-like Receptor 1 (human) (GD2.F4)
SYNONYMS	anti-TLR1 (human) MAb (GD2.F4) anti-CD281 (human) MAb (GD2.F4)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	TLR Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-130-C100		100 µg	340.00 USD
ALX-804-130PF-C100	Preservative Free	100 µg	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	GD2.F4
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml
FORMULATION:	GD2.F4: Liquid. In PBS containing 0.02% sodium azide. GD2.F4 (preservative free): Liquid. In PBS.
IMMUNOGEN:	Recombinant human soluble TLR1 (Toll-like receptor 1) extracellular domain.
SPECIFICITY:	Recognizes human TLR1. Does not cross-react with human TLR2 or TLR4.
APPLICATION:	Flow Cytometry (5-15µg/ml) Incubate with antibody at 37°C and then on ice with GAM-FITC.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored as recommended.
HANDLING:	Avoid freeze/thaw cycles.

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Product Specific Literature References

Evidence for an accessory protein function for Toll-like receptor 1 in anti-bacterial responses: D.H. Wyllie, et al.; J. Immunol. 165, 7125 (2000) Abstract; Full Text
Regulation of Toll-like receptors in human monocytes and dendritic cells: A. Visintin, et al.; J. Immunol. 166, 249 (2001) Abstract; Full Text

General Information

MANUFACTURER

Manufactured by Apotech Corporation.

Related Products

ALX-211-200	Polyclonal Antibody to Mouse IgG1 (Human & Mouse Ig adsorbed) (FITC)
ALX-211-202	Polyclonal Antibody to Mouse IgG1 (Human & Mouse Ig adsorbed) (Biotin)
ALX-211-201	Polyclonal Antibody to Mouse IgG1 (Human & Mouse Ig adsorbed) (R-PE)

Further Categories Containing This Product:

Monoclonal Antibodies • CD Antibodies

ALX-804-323

Revised 31-Jul-07

Monoclonal Antibody to Toll-like Receptor 2 (human) (TL2.1)
SYNONYMS	anti-TLR2 (human) MAb (TL2.1) anti-Toll/Interleukin-1 Receptor-like Gene 4 (human) MAb (TL2.1) anti-TIL4 (human) MAb (TL2.1) anti-CD282 (human) MAb (TL2.1)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	TLR Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-323-C050		50 µg	230.00 USD
ALX-804-323PF-C050	Preservative Free	50 µg	230.00 USD
ALX-804-323PF-C100	Preservative Free	100 µg	290.00 USD
ALX-804-323F-T100	FITC	100 tests	390.00 USD
ALX-804-323TD-T100	ATTO 488	100 tests	395.00 USD
ALX-804-323TS-T100	ATTO 647N	100 tests	395.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	TL2.1
ISOTYPE:	Mouse IgG2a
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	TL2.1: Liquid. In PBS containing 0.02% sodium azide. TL2.1PF: Liquid. In PBS. TL2.1-ATTO 488: Liquid. In PBS containing 0.02% sodium azide. TL2.1-ATTO 647N: Liquid. In PBS containing 0.02% sodium azide. TL2.1-FITC: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	CHO cells stably transfected with human TLR2 (Toll-like receptor 2).
SPECIFICITY:	Recognizes human TLR2. Does not cross-react with human TLR1 or TLR4.
APPLICATION:	TL2.1: ELISA Flow Cytometry [2, 4]: Incubate with 37°C (1:10-1:100) and then on ice with GAM-FITC. Immunohistochemistry Immunoprecipitation TL2.1 (Preservative Free): ELISA Flow Cytometry [2, 4]: Incubate with 37°C (1:10-1:100) and then on ice with GAM-FITC. Immunohistochemistry Immunoprecipitation Functional Application (blocking) [1, 5-7]: Use preservative free formulation. TL2.1-ATTO 488: Flow Cytometry (1:100) TL2.1-ATTO 647N: Flow Cytometry (1:100) TL2.1-FITC: Flow Cytometry [2, 4]
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored as recommended.
HANDLING:	For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep ATTO, Biotin- and FITC-conjugated formats at +4°C. Do not freeze. Protect from light.

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Product Description

New ATTO-fluorescent antibodies show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 488 shows bright green fluorescence (λabs (max): 501nm, λem (max): 523nm, εmax: 90’000).
Licenced product covered by US Patent 7,071,310. Sold with permission of Leiv Eiriksson Nyskaping AS.

Product Specific Literature References

[1] Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products: E. Lien, et al.; J. Biol. Chem. 274, 33419 (1999) Abstract; Full Text
[2] Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide: T.H. Flo, et al.; J. Immunol. 164, 2064 (2000) Abstract; Full Text
[3] Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation: E. Faure, et al.; J. Immunol. 166, 2018 (2001) Abstract; Full Text
[4] Differential expression of Toll-like receptor 2 in human cells: T.H. Flo, et al.; J. Leukoc. Biol. 69, 474 (2001) Abstract
[5] A novel synthetic acyclic lipid A-like agonist activates cells via the lipopolysaccharide/toll-like receptor 4 signaling pathway: E. Lien, et al.; J. Biol. Chem. 276, 1873 (2001) Abstract; Full Text
[6] Monocytic cell activation by Nonendotoxic glycoprotein from Prevotella intermedia ATCC 25611 is mediated by toll-like receptor 2: S. Sugawara, et al.; Infect. Immun. 69, 4951 (2001) Abstract
[7] Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae: J.E. Wang, et al.; Infect. Immun. 69, 2402 (2001) Abstract; Full Text
[8] Toll-like receptor (TLR)2 and TLR4 in human peripheral blood granulocytes: a critical role for monocytes in leukocyte lipopolysaccharide responses: I. Sabroe, et al.; J. Immunol. 168, 4701 (2002) Abstract
[9] Two lipoproteins extracted from Escherichia coli K-12 LCD25 lipopolysaccharide are the major components responsible for Toll-like receptor 2-mediated signaling: H.K. Lee, et al.; J. Immunol. 168, 4012 (2002) Abstract; Full Text
[10] A novel host-parasite lipid cross-talk. Schistosomal lyso-phosphatidylserine activates toll-like receptor 2 and affects immune polarization: D. van der Kleij, et al.; J. Biol. Chem. 277, 48122 (2002) Abstract; Full Text
[11] Dysregulation of LPS-induced Toll-like receptor 4-MyD88 complex formation and IL-1 receptor-associated kinase 1 activation in endotoxin-tolerant cells: A.E. Medvedev, et al.; J. Immunol. 169, 5209 (2002) Abstract; Full Text
[12] Lipopolysaccharide rapidly traffics to and from the Golgi apparatus with the toll-like receptor 4-MD-2-CD14 complex in a process that is distinct from the initiation of signal transduction: E. Latz, et al.; J. Biol. Chem. 277, 47834 (2002) Abstract; Full Text
[13] Haemophilus influenzae porin induces Toll-like receptor 2-mediated cytokine production in human monocytes and mouse macrophages: M. Galdiero, et al.; Infect. Immun. 72, 1204 (2004) Abstract; Full Text
[14] Divergent trophoblast responses to bacterial products mediated by TLRs: V.M. Abrahams, et al.; J. Immunol. 173, 4286 (2004) Abstract
[15] The role of TLR2 in the inflammatory activation of mouse fibroblasts by human antiphospholipid antibodies: N. Satta, et al.; Blood 109, 1507 (2007) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION	TL2.1-FITC: 1 test means: 1µl of a 0.1mg/ml stock solution were used to stain 200’000 cells in a sample volume of 50µl.
MANUFACTURER	Manufactured by Apotech Corporation.

Further Categories Containing This Product:

Monoclonal Antibodies • CD Antibodies

ALX-804-324

Revised 09-May-07

Monoclonal Antibody to Toll-like Receptor 2 (human) (TL2.3)
SYNONYMS	anti-TLR2 (human) MAb (TL2.3) anti-Toll/Interleukin-1 Receptor-like Gene 4 (human) MAb (TL2.3) anti-TIL4 (human) MAb (TL2.3) anti-CD282 (human) MAb (TL2.3)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	TLR Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-324-C050		50 µg	230.00 USD
ALX-804-324PF-C100	Preservative Free	100 µg	290.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	TL2.3
ISOTYPE:	Mouse IgG2a
CONCENTRATION:	1mg/ml
FORMULATION:	TL2.3: Liquid. In PBS containing 0.02% sodium azide. TL2.3 (preservative free): Liquid. In PBS.
IMMUNOGEN:	CHO cells stably transfected with human TLR2 (Toll-like receptor 2).
SPECIFICITY:	Recognizes human TLR2. Does not cross-react with human TLR1 or TLR4.
APPLICATION:	ELISA Immunocytochemistry Immunohistochemistry (frozen sections)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored as recommended.
HANDLING:	Avoid freeze/thaw cycles.

Product Images

Please click on thumbnails to enlarge.

Product Description

Licenced product covered by US Patent 7,071,310. Sold with permission of Leiv Eiriksson Nyskaping AS.

Product Specific Literature References

Differential expression of Toll-like receptor 2 in human cells: T.H. Flo, et al.; J. Leukoc. Biol. 69, 474 (2001) Abstract
The extracellular toll-like receptor 2 domain directly binds peptidoglycan derived from Staphylococcus aureus: D. Iwaki, et al.; J. Biol. Chem. 277, 24315 (2002) Abstract; Full Text
Cutting edge: link between innate and adaptive immunity: Toll-like receptor 2 internalizes antigen for presentation to CD4+ T cells and could be an efficient vaccine target: K.W. Schjetne, et al.; J. Immunol. 171, 32 (2003) Abstract; Full Text

General Information

MANUFACTURER

Manufactured by Apotech Corporation.

Further Categories Containing This Product:

Monoclonal Antibodies • CD Antibodies

ALX-804-362

Revised 25-Sep-08

Monoclonal Antibody to Toll-like Receptor 3 (human) (40C1285.6)
SYNONYMS	anti-TLR3 MAb (40C1285.6) anti-CD283 (human) MAb (40C1285.6)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	TLR Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-362-C100		100 µg	293.00 USD
ALX-804-362B-C100	Biotin	100 µg	319.00 USD
ALX-804-362F-C100	FITC	100 µg	358.00 USD
ALX-804-362R-C100	R-PE	100 µg	397.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	40C1285.6
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.5mg/ml
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	40C1285.6: Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide. 40C1285.6-Biotin: Liquid. In PBS containing 0.02% sodium azide. 40C1285.6-FITC: Liquid. In 10mM TRIS containing 150mM sodium chloride and 0.02% sodium azide. 40C1285.6-R-PE: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptides corresponding to human TLR3 (Toll-like receptor 3) cytoplasmic portion.
SPECIFICITY:	Recognizes human TLR3.
APPLICATION:	Flow Cytometry (0.5-4µg/10⁶ cells for intracellular staining, for cell surface see [7]) Immunocytochemistry (see [3],[8] and [9]) Immunohistochemistry (paraffin sections) (see [4] and [6]) Immunoprecipitation (2µg/10⁶ transfected cells) Western Blot (1-3µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
HANDLING:	For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep Biotin-, FITC- and R-PE-conjugated formats at +4°C. Do not freeze. Protect from light.

Product Images

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Product Specific Literature References

[1] Subcellular localization of Toll-like receptor 3 in human dendritic cells: M. Matsumoto, et al.; J. Immunol. 171, 3154 (2003) Abstract
[2] The effect of innate immunity on autoimmune diabetes and the expression of Toll-like receptors on pancreatic islets: L. Wen, et al.; J. Immunol. 172, 3173 (2004) Abstract
[3] Regulation of the polymeric Ig receptor by signaling through TLRs 3 and 4: linking innate and adaptive immune responses: T.A. Schneeman, et al.; J. Immunol. 175, 376 (2005) Abstract
[4] Toll-like receptors-2, -3 and -4 expression patterns on human colon and their regulation by mucosal-associated bacteria: E. Furrie, et al.; Immunology 115, 565 (2005) Abstract
[5] Distinct poly(I-C) and virus-activated signaling pathways leading to interferon-beta production in hepatocytes: K. Li, et al.; J. Biol. Chem. 280, 16739 (2005 280, 16739 (2005) Abstract; Full Text
[6] Thyrocytes express a functional toll-like receptor 3: overexpression can be induced by viral infection and reversed by phenylmethimazole and is associated with Hashimoto’s autoimmune thyroiditis: N. Harii, et al.; Mol. Endocrinol. 19, 1231 (2005) Abstract
[7] Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils: C.K. Wong, et al.; Am. J. Respir. Cell Mol. Biol. 37, 85 (2007) Abstract
[8] Biochemical and functional analyses of the human Toll-like receptor 3 ectodomain: C.T. Ranjith-Kumar, et al.; J. Biol. Chem. 282, 7668 (2007) Abstract; Full Text
[9] Effects of single nucleotide polymorphisms on Toll-like receptor 3 activity and expression in cultured cells: C.T. Ranjith-Kumar, et al.; J. Biol. Chem. 282, 17696 (2007) Abstract; Full Text

Further Categories Containing This Product:

Antiviral Signalling / Related Products • Monoclonal Antibodies • CD Antibodies

ALX-804-474

Revised 24-Mar-05

Monoclonal Antibody to Toll-like Receptor 3 (human) (TLR3.7)
SYNONYMS	anti-TLR3 (human) MAb (TLR3.7) anti-CD283 (human) MAb (TLR3.7)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	TLR Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-474-C050		50 µg	220.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	TLR3.7
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.2mg/ml.
FORMULATION:	Liquid. Purified antibody in PBS containing 10% glycerol. Does not contain any preservatives.
IMMUNOGEN:	Recombinant human TLR3 (Toll-like receptor 3).
SPECIFICITY:	Recognizes human TLR3.
APPLICATION:	Flow Cytometry Immunocytochemistry Immunoprecipitation Functional Application (blocks double-stranded RNA-mediated signalling)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored as recommended.
HANDLING:	Avoid freeze/thaw cycles.

Product Images

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Product Specific Literature References

Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling: M. Matsumoto, et al.; BBRC 293, 1364 (2002) Abstract
Subcellular localization of Toll-like receptor 3 in human dendritic cells: M. Matsumoto, et al.; J. Immunol. 171, 3154 (2003) Abstract
Mechanism of up-regulation of human Toll-like receptor 3 secondary to infection of measles virus-attenuated strains: M. Tanabe, et al.; BBRC 311, 39 (2003) Abstract

General Information

MANUFACTURER

Manufactured by Apotech Corporation.

Further Categories Containing This Product:

Antiviral Signalling / Related Products • CD Antibodies • Monoclonal Antibodies

ALX-804-419

Revised 09-Jul-07

Monoclonal Antibody to Toll-like Receptor 4 (human) (HTA125)
SYNONYMS	anti-TLR4 (human) MAb (HTA125) anti-CD284 (human) MAb (HTA125)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	TLR Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-419-C100		100 µg	270.00 USD
ALX-804-419PF-C100	Preservative Free	100 µg	270.00 USD
ALX-804-419F-T100	FITC	100 tests	300.00 USD
ALX-804-419TD-T100	ATTO 488	100 tests	340.00 USD
ALX-804-419TS-T100	ATTO 647N	100 tests	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	HTA125
ISOTYPE:	Mouse IgG2a
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide. HTA125 (preservative free): Liquid. In PBS.
IMMUNOGEN:	Ba/F3 cell line expressing human TLR4 (Toll-like receptor 4).
SPECIFICITY:	Recognizes human TLR4 cell surface antigen.
APPLICATION:	HTA125: Flow Cytometry: Incubate at 37°C (1:10-1:100) and then on ice with GAM-FITC. Immunoprecipitation HTA125 (preservative free): Flow Cytometry: Incubate at 37°C (1:10-1:100) and then on ice with GAM-FITC. Immunoprecipitation Functional Application (blocks activation of monocytes with LPS; use preservative free formulation). HTA125-ATTO 488: Flow Cytometry (1:100) HTA125-ATTO 647N: Flow Cytometry HTA125-FITC: Flow Cytometry Immunoprecipitation Note: Not suitable for Western blot (does not detect denatured protein).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored as recommended.
HANDLING:	For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep ATTO, Biotin- and FITC-conjugated formats at +4°C. Do not freeze. Protect from light.

Product Images

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Product Specific Literature References

MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4: R. Shimazu, et al.; J. Exp. Med. 189, 1777 (1999) Abstract; Full Text
Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2: S. Akashi, et al.; BBRC 268, 172 (2000) Abstract
Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-κB: Q. Jiang, et al.; J. Immunol. 165, 3541 (2000) Abstract; Full Text
Toll-like receptors confer responsiveness to lipopolysaccharide from Porphyromonas gingivalis in human gingival fibroblasts: K. Tabeta, et al.; Infect. Immun. 68, 3731 (2000) Abstract; Full Text
Induction of cytokine production in human T cells and monocytes by highly purified lipoteichoic acid: involvement of Toll-like receptors and CD14: E. Ellingsen, et al.; Med. Sci. Monitor 8, BR149 (2002) Abstract; Full Text
Lipopolysaccharide rapidly traffics to and from the Golgi apparatus with the toll-like receptor 4-MD-2-CD14 complex in a process that is distinct from the initiation of signal transduction: E. Latz, et al.; J. Biol. Chem. 277, 47834 (2002) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION	1 test means: 1µl of MAb is used to stain 500’000 cells in a sample volume of 50µl.
MANUFACTURER	Manufactured by Apotech Corporation.

Further Categories Containing This Product:

CD Antibodies • Monoclonal Antibodies

ALX-804-430

Revised 24-Apr-07

Monoclonal Antibody to Toll-like Receptor 4/MD-2 (mouse) (MTS510)
SYNONYMS	anti-TLR4/MD-2 (mouse) MAb (MTS510)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	TLR Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-430PF-C100	Preservative Free	100 µg	270.00 USD
ALX-804-430-C100	Purified	100 µg	270.00 USD
ALX-804-430F-T100	FITC	100 tests	300.00 USD
ALX-804-430TD-T100	ATTO 488	100 tests	340.00 USD
ALX-804-430TS-T100	ATTO 647N	100 tests	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Mouse
CLONE:	MTS510
ISOTYPE:	Rat IgG2a
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide. MTS510 (Preservative Free): Liquid. In PBS.
IMMUNOGEN:	Ba/F3 cells expressing mouse TLR4 and MD-2 complex.
SPECIFICITY:	Recognizes the mouse TLR4/MD-2 complex.
APPLICATION:	MTS510 & MTS510 (Preservative Free): Flow Cytometry Immunoprecipitation Functional Application (inhibition of LPS-induced cytokine production; use preservative free formulation). MTS510-ATTO 488: Flow Cytometry MTS510-ATTO 647N: Flow Cytometry MTS510-FITC: Flow Cytometry