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APO-54N-025
Revised 08-Apr-08
IL-33, Soluble (human) Detection Set [For ELISA Application]
SYNONYMS
Interleukin-33, Soluble (human) Detection Set [For ELISA Application]
NF-HEV, Soluble (human) Detection Set [For ELISA Application]
PRODUCT LINE
Chemokines & Cytokines
PRODUCT CATEGORY
Interleukin Kits & Sets
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
APO-54N-025-KI01
1 Set
630.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY:
5pg/ml (range 0 to 500pg/ml).
QUANTITY:
For 5x96 wells.
KIT/SET CONTAINS:
1 vial Standard (lyophilized) (STD).
1 vial Coating Antibody (COAT).
1 vial Detection Antibody (DET).
APPLICATION:
For the quantitative determination of soluble human IL-33 from biological fluids (serum and cell culture supernatant). Does not cross-react with mouse IL-33.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
Interleukin-1 (IL-1) family members, such as IL-1α/β and IL-18 play important roles in host defense, immune regulation, neuronal injury and inflammation [1, 2]. Recently, a new member of this family, IL-33, also named Nuclear Factor from High Endothelial Venules (NF-HEV) [3], has been reported to be the specific ligand for ST2 [4], an orphan receptor of the IL-1 receptor family [5]. Structurally, IL-33 is closest to IL-18, rather than IL-1β. The human IL-33 is expressed with a prodomain (aa 1-111) that is cleaved by caspase-1 to produce a 18kDa mature protein (112-270) [6]. The stimulation of ST2 by IL-33 induces the NF-κB and MAPK athways. IL-33 is synthesized in response to cytokines such as IL-1β (but also TNFα) and plays an important role in T
H
2-associated immunology, by triggering an increase secretion of IL-5 and IL-13 from polarized T
H
2 cells. Overexpression of IL-33 in mice leads to severe pathological changes in mucosal organs.
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link Q2YEJ5:
Interleukin-33 (human)
MANUFACTURER
Manufactured by Apotech Corporation.
General Literature References
[1]
Differences in signaling pathways by IL-1beta and IL-18:
J.K. Lee, et al. ; Proc. Natl. Acad. Sci.
101
, 8815 (2004)
Abstract
[2]
Interleukin-1 and neuronal injury:
S.M. Allan, et al.; Nat. Rev. Immunol.
5
, 629 (2005)
Abstract
[3]
Molecular characterization of NF-HEV, a nuclear factor preferentially expressed in human high endothelial venules:
E.S. Baekkevold, et al. ; Am. J. Pathol.
163
, 69 (2003)
Abstract
[4]
IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines:
J. Schmitz, et al.; Immunity
23
, 479 (2005)
Abstract
[5]
ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance:
E.K. Brint, et al. ; Nat. Immunol.
5
, 373 (2005)
Abstract
[6]
Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases:
F. Martinon & J. Tschopp; Cell
117
, 561 (2004)
Abstract
Further Categories Containing This Product:
Interleukin-33 [IL-33] & Receptors/Related Products
•
ELISA Sets
ALX-201-363
Revised 18-Mar-08
IL-33 (human) (recombinant)
SYNONYMS
Interleukin-33 (human) (recombinant)
NF-HEV (human) (recombinant)
PRODUCT LINE
Chemokines & Cytokines
PRODUCT CATEGORY
Interleukins
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-363-C010
10 µg
295.00 USD
ALX-201-363-C050
50 µg
795.00 USD
Product Specification
SOURCE/HOST:
Produced in HEK 293 cells. Recombinant human IL-33 (aa 112-270) is fused at the C-terminus to a FLAG
®
-tag.
CONCENTRATION:
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Liquid. 0.2µm-filtered solution in PBS, pH 7.2.
ENDOTOXIN CONTENT:
<0.1EU/µg protein (LAL-test).
SPECIFICITY:
Binds to human ST2.
BIOLOGICAL ACTIVITY:
Activates ST2-dependent NF-κB pathway.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link Q2YEJ5:
Interleukin-33 (human) (recombinant)
FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER
Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
•
Interleukin-33 [IL-33] & Receptors/Related Products
ALX-522-098
Revised 27-Mar-08
IL-33 (human) (recombinant)
SYNONYMS
Interleukin-33 (human) (recombinant)
NF-HEV (human) (recombinant)
PRODUCT LINE
Chemokines & Cytokines
PRODUCT CATEGORY
Interleukins
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-522-098-C010
10 µg
190.00 USD
ALX-522-098-3010
SuperPack
3x10 µg
410.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
MW:
~18kDa
SOURCE/HOST:
Produced in
E. coli
. Untagged human IL-33 (aa 112-270).
CONCENTRATION:
0.1mg/ml after reconstitution.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:
<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:
Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY:
Binds to human ST2.
BIOLOGICAL ACTIVITY:
Activates human ST2-dependent NF-κB pathway. Does not activate mouse ST2-dependent NF-κB.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Specific Literature References
IL-33, the IL-1-like cytokine ligand for ST2 receptor, is a chromatin-associated nuclear factor in vivo:
V. Carriere et al.; PNAS
104
, 282 (2007)
Abstract
General Information
IL-33 is a new member of the IL-1 family. It is involved in T helper type 2 response via the activation of its receptor ST2 (IL-1 R4) driving the production of Th2-associated cytokines. The stimulation of ST2 by IL-33 induces the NF-κB and MAPK pathways. The human IL-33 protein is expressed with a prodomain (aa 1-111) which is cleaved by caspase-1 to produce the 18kDa mature protein (112-270).
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link Q2YEJ5:
Interleukin-33 (human)
MANUFACTURER
Manufactured by Apotech Corporation.
General Literature References
IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines:
J. Schmitz, et al.; Immunity
23
, 479 (2005)
Abstract
Related Products
ALX-804-856
Monoclonal Antibody to IL-33 (Nessy-2)
APO-54N-025
IL-33, Soluble (human) Detection Set [For ELISA Application]
ALX-804-840
Monoclonal Antibody to IL-33 (Nessy-1)
ALX-210-933
Polyclonal Antibody to IL-33 (AT110)
ALX-522-101
IL-33 (mouse) (recombinant)
ALX-522-125
IL-33 (rat) (recombinant)
Further Categories Containing This Product:
Interleukin-33 [IL-33] & Receptors/Related Products
•
Recombinant Proteins/Fusion Proteins
ALX-201-350
Revised 18-Mar-08
IL-33 (human) (recombinant) (His)
SYNONYMS
Interleukin-33 (human) (recombinant) (His)
NF-HEV (human) (recombinant) (His)
PRODUCT LINE
Chemokines & Cytokines
PRODUCT CATEGORY
Interleukins
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-350-C010
10 µg
295.00 USD
ALX-201-350-C050
50 µg
795.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
. Recombinant human IL-33 (aa 112-270) is fused at the C-terminus to a His-tag.
CONCENTRATION:
1mg/ml
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Liquid. 0.2µm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing
150mM sodium chloride.
ENDOTOXIN CONTENT:
<1.0EU/µg protein (LAL-test).
SPECIFICITY:
Binds to human ST2.
BIOLOGICAL ACTIVITY:
Activates ST2-dependent NF-κB pathway.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link Q2YEJ5:
Interleukin-33 (human) (recombinant)
MANUFACTURER
Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
•
Interleukin-33 [IL-33] & Receptors/Related Products
ALX-522-101
Revised 27-Mar-08
IL-33 (mouse) (recombinant)
SYNONYMS
Interleukin-33 (mouse) (recombinant)
NF-HEV (mouse) (recombinant)
PRODUCT LINE
Chemokines & Cytokines
PRODUCT CATEGORY
Interleukins
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-522-101-C010
10 µg
190.00 USD
ALX-522-101-3010
SuperPack
3x10 µg
410.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW:
~18kDa
SOURCE/HOST:
Produced in
E. coli
. Untagged mouse IL-33 (aa 109-266).
CONCENTRATION:
0.1mg/ml after reconstitution.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:
<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:
Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY:
Binds to mouse and human ST2.
BIOLOGICAL ACTIVITY:
Activates mouse and human ST2-dependent NF-κB pathway. Induces IL-6 production in P815 mastocytoma cells and in bone marrow derived mast cells.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
IL-1 receptor accessory protein is essential for IL-33-induced activation of T lymphocytes and mast cells:
S. Ali, et al.; PNAS
104
, 18660 (2007)
Abstract
General Information
IL-33 is a new member of the IL-1 family. It is involved in T helper type 2 response via the activation of its receptor ST2 (IL-1 R4) driving the production of Th2-associated cytokines. The stimulation of ST2 by IL-33 induces the NF-κB and MAPK pathways. The mouse IL-33 protein is expressed with a prodomain (aa 1-108) which is cleaved to produce the 18kDa mature protein (109-266).
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link Q2YEJ4:
Interleukin-33 (mouse)
MANUFACTURER
Manufactured by Apotech Corporation.
General Literature References
IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines:
J. Schmitz, et al.; Immunity
23
, 479 (2005)
Abstract
Related Products
ALX-804-856
Monoclonal Antibody to IL-33 (Nessy-2)
ALX-804-840
Monoclonal Antibody to IL-33 (Nessy-1)
ALX-522-125
IL-33 (rat) (recombinant)
ALX-210-933
Polyclonal Antibody to IL-33 (AT110)
ALX-522-098
IL-33 (human) (recombinant)
APO-54N-025
IL-33, Soluble (human) Detection Set [For ELISA Application]
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
•
Interleukin-33 [IL-33] & Receptors/Related Products
ALX-201-361
Revised 18-Mar-08
IL-33 (mouse) (recombinant) (His)
SYNONYMS
Interleukin-33 (mouse) (recombinant) (His)
NF-HEV (mouse) (recombinant) (His)
PRODUCT LINE
Chemokines & Cytokines
PRODUCT CATEGORY
Interleukins
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-361-C010
10 µg
295.00 USD
ALX-201-361-C050
50 µg
795.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
. Recombinant mouse IL-33 (aa 109-266) is fused at the C-terminus to a His-tag.
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Liquid. 0.2µm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing
150mM sodium chloride.
ENDOTOXIN CONTENT:
<1EU/µg protein (LAL-test).
SPECIFICITY:
Binds to human ST2.
BIOLOGICAL ACTIVITY:
Activates ST2-dependent NF-κB pathway.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link Q2YEJ4:
Interleukin-33 (mouse) (recombinant)
MANUFACTURER
Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
•
Interleukin-33 [IL-33] & Receptors/Related Products
ALX-522-125
Revised 27-Mar-08
IL-33 (rat) (recombinant)
SYNONYMS
Interleukin-33 (rat) (recombinant)
PRODUCT LINE
Chemokines & Cytokines
PRODUCT CATEGORY
Interleukins
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-522-125-C010
10 µg
290.00 USD
Product Specification
MW:
~16kDa
SOURCE/HOST:
Produced in
E. coli
. Untagged rat IL-33 (aa 109-264).
CONCENTRATION:
0.1mg/ml after reconstitution.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:
<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:
Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
General Information
IL-33 is a new member of the IL-1 family. It is involved in T helper type 2 response via the activation of its receptor ST2 (IL-1 R4) driving the production of Th2-associated cytokines. The stimulation of ST2 by IL-33 induces the NF-κB and MAPK pathways. The human IL-33 protein is expressed with a prodomain (aa 1-111) which is cleaved by caspase-1 to produce the 18kDa mature protein (112-270).
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link Q66H70:
Interleukin-33 (rat)
MANUFACTURER
Manufactured by Apotech Corporation.
General Literature References
IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines:
J. Schmitz, et al.; Immunity
23
, 479 (2005)
Abstract
IL-33 and ST2 comprise a critical biomechanically induced and cardioprotective signaling system:
S. Sanada, et al.; J. Clin. Invest.
117
, 1538 (2007)
Abstract
;
Full Text
Related Products
ALX-522-101
IL-33 (mouse) (recombinant)
ALX-522-098
IL-33 (human) (recombinant)
ALX-210-933
Polyclonal Antibody to IL-33 (AT110)
ALX-804-840
Monoclonal Antibody to IL-33 (Nessy-1)
ALX-804-856
Monoclonal Antibody to IL-33 (Nessy-2)
APO-54N-025
IL-33, Soluble (human) Detection Set [For ELISA Application]
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
•
Interleukin-33 [IL-33] & Receptors/Related Products
ALX-804-726
Revised 02-Aug-07
Monoclonal Antibody to IL-33 (human) (IL33305B)
SYNONYMS
anti-Interleukin-33 (human) MAb (IL33305B)
anti-NF-HEV (human) MAb (IL33305B)
PRODUCT LINE
Chemokines & Cytokines
PRODUCT CATEGORY
Interleukin Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-726-C050
50 µg
195.00 USD
ALX-804-726-C100
100 µg
360.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
IL33305B
ISOTYPE:
Mouse IgG2a
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:
Recombinant human IL-33 (interleukin-33).
SPECIFICITY:
Recognizes human IL-33. Does not cross-react with mouse IL-33.
APPLICATION:
ELISA
(direct and indirect: 1:2’000-1:5’000)
Western Blot
(1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Immunoprecipitation
Functional Application
(Neutralization)
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 1-2 weeks when stored at +4°C.
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
MANUFACTURER
Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Interleukin-33 [IL-33] & Receptors/Related Products
•
Polyclonal Antibodies
ALX-804-728
Revised 23-Aug-07
Monoclonal Antibody to IL-33 (IL33026B)
SYNONYMS
anti-Interleukin-33 MAb (IL33026B)
anti-NF-HEV MAb (IL33026B)
PRODUCT LINE
Chemokines & Cytokines
PRODUCT CATEGORY
Interleukin Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-728-C050
50 µg
195.00 USD
ALX-804-728-C100
100 µg
360.00 USD
