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Fas Signalling
You are here: Product Lines > Cell Death / Apoptosis / Autophagy > Fas Signalling
 
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ALX-748-015 Revised 24-Mar-05
Fas (human) cDNA Probe
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SYNONYMS CD95 (human) cDNA Probe
APO-1 (human) cDNA Probe
TNFRSF 6 (human) cDNA Probe
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Fas cDNA Probes
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-748-015-C001   1 µg 140.00 USD Add To Cart
Product Specification
SOURCE/HOST: Derived from the coding region of the human Fas (CD95) gene. Has a length of ~500bp located within the region between nucleotides 500-1000.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (agarose gel electrophoresis)
FORMULATION: Lyophilized powder.
RECONSTITUTION: Reconstitute with 10µl sterile water or 10mM Tris . HCl containing 0.1mM Na2EDTA.
APPLICATION: Northern Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
DNA & RNA Probes / Related Products
 
 
ALX-522-002 Revised 06-Jun-07
Fas (human):Fc (human) (recombinant)
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SYNONYMS APO-1 (human):Fc (human) (recombinant)
CD95 (human):Fc (human) (recombinant)
TNFRSF 6 (human):Fc (human) (recombinant)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Fas
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-002-C050   50 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human Fas (CD95; APO-1) (aa 7-154) is fused to the Fc portion of human IgG1.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions can be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds human, mouse and rat FasL (APO-1L; CD95L; CD178).
BIOLOGICAL ACTIVITY: Inhibits the activity of human and mouse soluble Fas (sFasL) and thereby sFasL-mediated lysis of Fas sensitive cells in a concentration range of 20-100µg/ml. When used with cross-linking enhancer (see Set Prod. No. ALX-850-016), the inhibitory activity of rhFas:Fc is increased approximately 20- to 50-fold. Concentrations of rhFas:Fc required to inhibit may vary depending on the cell viability and on the concentrations of sFasL used to kill the cells.
APPLICATION: ELISA (capture, 1µg/ml).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
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Product Specific Literature References
Characterization of Fas (Apo-1, CD95)-Fas ligand interaction: P. Schneider, et al.; J. Biol. Chem. 272, 18827 (1997) Abstract; Full Text
Cysteine 230 is essential for the structure and activity of the cytotoxic ligand TRAIL: J.L. Bodmer, et al.; J. Biol. Chem. 275, 20632 (2000) Abstract; Full Text
Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000) Abstract
TGF-beta induces the expression of the FLICE-inhibitory protein and inhibits Fas-mediated apoptosis of microglia: R. Schlapbach, et al.; Eur. J. Immunol. 30, 3680 (2000) Abstract
Mechanisms underlying neuronal death induced by chromogranin A-activated microglia: J. Ciesielski-Treska, et al.; J. Biol. Chem. 276, 13113 (2001) Abstract; Full Text
T cell costimulation by the TNF ligand BAFF: B. Huard et al.; J. Immunol. 167, 6225 (2001) Abstract
Productive HIV-1 infection of primary CD4+ T cells induces mitochondrial membrane permeabilization leading to a caspase-independent cell death: F. Petit et al; J. Biol. Chem. 277, 1477 (2002) Abstract; Full Text
Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex: N. Holler, et al.; Mol. Cell. Biol. 23, 1428 (2003) Abstract; Full Text
High expression of Fas ligand by synovial fluid-derived gamma delta T cells in Lyme arthritis: K. Roessner, et al.; J. Immunol. 170, 2702 (2003) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P25445: Fas (human) (precursor)
AfCS Signalling Gateway link A000600: Fas (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-850-016 Revised 23-Apr-08
Fas (human):Fc (human) (recombinant) Set
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SYNONYMS CD95 (human):Fc (human) (recombinant) Set
TNFRSF 6 (human):Fc (human) (recombinant) Set
APO-1 (human):Fc (human) (recombinant) Set
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Fas Kits & Sets
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-016-KI01   1 Set 460.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human Fas (aa 7-154) is fused to the Fc portion of human IgG1.
CONCENTRATION: 1mg/ml after reconstitution.
KIT/SET CONTAINS: 50µg of Fas (human):Fc (human) (recombinant) (Prod. No. ALX-522-002) and 20µg of Enhancer for Receptors (Prod. No. ALX-203-001), which increases the inhibitory activity by ~20- to 50-fold to a concentration range of 0.5-5µg/ml.
PURITY: ≥95% (SDS-PAGE).
FORMULATION: Lyophilized. Contains PBS.
RECONSTITUTION: Reconstitute Fas:Fc with 50µl and enhancer with 20µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Fas:Fc binds human, mouse and rat FasL (APO-1L; CD95L; CD178).
APPLICATION: Inhibits the activity of human and mouse soluble Fas ligand (sFasL) and thereby sFasL-mediated lysis of Fas sensitive cells. Concentration of rhFas:Fc required to inhibit may vary depending on the cell viability and the concentrations of sFasL used to kill the cells.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
TGF-beta induces the expression of the FLICE-inhibitory protein and inhibits Fas-mediated apoptosis of microglia: R. Schlapbach, et al.; Eur. J. Immunol. 30, 3680 (2000) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Enhancer for sFasL and rhFas:Fc are not identical and may show decreased efficiency when used together. We recommend to only use enhancer for rhFas:Fc.
Swiss-Prot link P25445: Fas (human) (precursor)
AfCS Signalling Gateway link A000600: Fas (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Monoclonal antibody-mediated tumor regression by induction of apoptosis: B.C. Trauth, et al.; Science 245, 301 (1989) Abstract
The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis: N. Itoh, et al.; Cell 66, 233 (1991) Abstract
Apoptosis mediated by the TNF-related cytokine and receptor families: C.F. Ware, et al.; J. Cell. Biochem. 60, 47 (1996) Abstract
Apoptosis by death factor: S. Nagata; Cell 88, 355 (1997) Abstract
Related Products
Further Categories Containing This Product:
Receptor / Ligand + Enhancer Set • CD Kits & Sets
 
 
ALX-748-014 Revised 24-Mar-05
FasL (human) cDNA Probe
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SYNONYMS APO-1L (human) cDNA Probe
CD95L (human) cDNA Probe
CD178 (human) cDNA Probe
TNFSF 6 (human) cDNA Probe
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY FasL cDNA Probes
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-748-014-C001   1 µg 140.00 USD Add To Cart
Product Specification
SOURCE/HOST: Derived from the coding region of the human FasL (APO-1L; CD95L; CD178) gene. Has a length of ~620bp located within the region between nucleotides 30-650.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (agarose gel electrophoresis)
FORMULATION: Lyophilized powder.
RECONSTITUTION: Reconstitute with 10µl sterile water or 10mM Tris . HCl containing 0.1mM Na2EDTA.
APPLICATION: Northern Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
DNA & RNA Probes / Related Products
 
 
ALX-522-001 Revised 10-Oct-08
FasL, Soluble (human) (recombinant)
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SYNONYMS APO-1L, Soluble (human) (recombinant)
CD95L, Soluble (human) (recombinant)
CD178, Soluble (human) (recombinant)
TNFSF 6, Soluble (human) (recombinant)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY FasL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-001-C010   10 µg 200.00 USD Add To Cart
ALX-522-001-3010 SuperPack 3x10 µg 410.00 USD Add To Cart
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Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
MW: ~32kDa (non-glycosylated), ~35kDa (glycosylated) (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG®-tag. Glycosylation of rhsFasL is similar to that of natural human FasL.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: Binds to human, mouse and rat Fas (CD95; APO-1).
BIOLOGICAL ACTIVITY: Kills Fas-sensitive cells at concentrations of >50ng/ml. Cross-linking enhancer (see Set Prod. No. ALX-850-014) increases the activity of rhsFasL approx. 50-fold. Attention: Results using sFasL may differ from those obtained with agonistic antibodies! (see [1]).
SPECIFIC ACTIVITY: ED50: 50ng/ml (A20 cells)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
[1] Agonist antibody and Fas ligand mediate different sensitivity to death in the signaling pathways of Fas and cytoplasmic mutants: A.R. Thilenius, et al.; Eur. J. Immunol. 27, 1108 (1997) Abstract
Characterization of Fas (Apo-1, CD95)-Fas ligand interaction: P. Schneider, et al.; J. Biol. Chem. 272, 18827 (1997) Abstract; Full Text
Mechanisms underlying neuronal death induced by chromogranin A-activated microglia: J. Ciesielski-Treska, et al.; J. Biol. Chem. 276, 13113 (2001) Abstract; Full Text
T cell costimulation by the TNF ligand BAFF: B. Huard, et al.; J. Immunol. 167, 6225 (2001) Abstract
Fas ligand overexpression on allograft endothelium inhibits inflammatory cell infiltration and transplant-associated intimal hyperplasia: M. Sata, et al.; J. Immunol. 166, 6964 (2001) Abstract
Fas-associated death domain protein (FADD) and caspase-8 mediate up-regulation of c-Fos by Fas ligand and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) via a FLICE inhibitory protein (FLIP)-regulated pathway: D. Siegmund, et al.; J. Biol. Chem. 276, 32585 (2001) Abstract; Full Text
BCR engagement induces Fas resistance in primary B cells in the absence of functional Bruton's tyrosine kinase: J.R. Tumang, et al.; J. Immunol. 168, 2712 (2002) Abstract
A second step of chemotaxis after transendothelial migration: keratinocytes undergoing apoptosis release IFN-gamma-inducible protein 10, monokine induced by IFN-gamma, and IFN-gamma-inducible alpha-chemoattractant for T cell chemotaxis toward epidermis in: S. Klunker, et al.; J. Immunol. 171, 1078 (2003) Abstract; Full Text
Cathepsin-B-dependent apoptosis triggered by antithymocyte globulins: a novel mechanism of T-cell depletion: M. C. Michallet, et al.; Blood 102, 3719 (2004) Abstract
The anti-apoptotic factor Bcl-2 can functionally substitute for the B cell survival but not for the marginal zone B cell differentiation activity of BAFF: A. Tardivel, et al.; Eur. J. Immunol. 34, 509 (2004) Abstract
Cytoskeleton-mediated death receptor and ligand concentration in lipid rafts forms apoptosis-promoting clusters in cancer chemotherapy: C. Gajate & F. Mollinedo; J. Biol. Chem. 280, 11641 (2005) Abstract; Full Text
Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development: V. Labi, et al.; J. Exp. Med. 205, 641 (2008) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P48023: FasL (human)
AfCS Signalling Gateway link A002830: FasL (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-850-014 Revised 23-Apr-08
FasL, Soluble (human) (recombinant) Set
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SYNONYMS APO-1L, Soluble (human) (recombinant) Set
CD95L, Soluble (human) (recombinant) Set
CD178, Soluble (human) (recombinant) Set
TNFSF 6, Soluble (human) (recombinant) Set
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY FasL Kits & Sets
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-014-KI02   1 Set 475.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG®-tag. Glycosylation of FasL, Soluble (human) (recombinant) is similar to natural human FasL.
CONCENTRATION: FasL: 0.1mg/ml after reconstitution
Enhancer: 1mg/ml after reconstitution
KIT/SET CONTAINS: 10µg of FasL, Soluble (human) (recombinant) (Prod. No. ALX-522-001) and 4x50µg of Enhancer for Ligands (Prod. No. ALX-804-034) which increases the biological activity (induction of apoptosis) by ~50-fold to a concentration range of 1-5ng/ml.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
RECONSTITUTION: Reconstitute FasL with 100µl and each vial of enhancer with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: FasL binds to human, mouse and rat Fas (CD95).
SPECIFIC ACTIVITY: ED50: 1ng/ml (A20 cells)
APPLICATION: Kills Fas-sensitive cells at concentrations of >1ng/ml in the presence of cross-linking enhancer.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Specific Literature References
Activation of Fas by FasL induces apoptosis by a mechanism that cannot be blocked by Bcl-2 or Bcl-x(L): D.C. Huang, et al.; PNAS 96, 14871 (1999) Abstract; Full Text
Fas engagement induces the maturation of dendritic cells (DCs), the release of interleukin (IL)-1beta, and the production of interferon gamma in the absence of IL-12 during DC-T cell cognate interaction: a new role for Fas ligand in in<: M. Rescigno, et al.; J. Exp. Med. 192, 1661 (2000) Abstract; Full Text
Fas ligand-induced c-Jun kinase activation in lymphoid cells requires extensive receptor aggregation but is independent of DAXX, and Fas-mediated cell death does not involve DAXX, RIP, or RAIDD: A. Villunger, et al.; J. Immunol. 165, 1337 (2000) Abstract; Full Text
Three adenovirus E3 proteins cooperate to evade apoptosis by tumor necrosis factor-related apoptosis-inducing ligand receptor-1 and -2: C.A. Benedict, et al.; J. Biol. Chem. 276, 3270 (2001) Abstract; Full Text
NF-κB signals induce the expression of c-FLIP: O. Micheau, et al.; Mol. Cell. Biol. 21, 5299 (2001) Abstract; Full Text
An essential role for membrane rafts in the initiation of Fas/CD95-triggered cell death in mouse thymocytes: A.O. Hueber, et al.; EMBO Rep. 3, 190 (2002) Abstract; Full Text
Potentiation of Fas-mediated apoptosis by an engineered glycosylphosphatidylinositol-linked Fas: P. Legembre, et al.; Cell Death Differ. 9, 329 (2002) Abstract; Full Text
Innate direct anticancer effector function of human immature dendritic cells. II. Role of TNF, lymphotoxin-alpha(1)beta(2), Fas ligand, and TNF-related apoptosis-inducing ligand: G. Lu, et al.; J. Immunol. 168, 1831 (2002) Abstract
Multiple pathways of TWEAK-induced cell death: M. Nakayama, et al.; J. Immunol. 168, 734 (2002) Abstract
Induction of apoptosis in malignant pleural mesothelioma cells by activation of the Fas (Apo-1/CD95) death-signal pathway: J.H. 4th Stewart et al.; J. Thorac. Cardiovasc. Surg. 123, 295 (2002) Abstract
Caspase-10 is recruited to and activated at the native TRAIL and CD95 death-inducing signalling complexes in a FADD-dependent manner but can not functionally substitute caspase-8: M.R. Sprick, et al.; EMBO J. 21, 4520 (2002) Abstract
Bcl-2 and Bcl-xL inhibit CD95-mediated apoptosis by preventing mitochondrial release of Smac/DIABLO and subsequent inactivation of X-linked inhibitor-of-apoptosis protein: X.M. Sun, et al.; J. Biol. Chem. 277, 11345 (2002) Abstract; Full Text
Hepatic natural killer cells exclusively kill splenic/blood natural killer-resistant tumor cells by the perforin/granzyme pathway: D. Vermijlen, et al.; J. Leukoc. Biol. 72, 668 (2002) Abstract
Intracellular localization of keratinocyte Fas ligand explains lack of cytolytic activity under physiological conditions: I. Viard-Leveugle, et al.; J. Biol. Chem. 278, 16183 (2003) Abstract; Full Text
Cutting edge: SDS-stable Fas microaggregates: an early event of Fas activation occurring with agonistic anti-Fas antibody but not with Fas ligand: P. Legembre, et al.; J. Immunol. 171, 5659 (2003) Abstract
The role of p53 and Fas in a model of acute murine graft-versus-host disease: S. Yada, et al.; J. Immunol. 174, 1291 (2005) Abstract; Full Text
CD1d-unrestricted human NKT cells release chemokines upon Fas engagement: M. Giroux and F. Denis; Blood 105, 703 (2005) Abstract; Full Text
Retinoic acid inhibits the proliferative response induced by CD40 activation and interleukin-4 in mantle cell lymphoma: M. Guidoboni, et al.; Cancer Res. 65, 587 (2005) Abstract; Full Text
Amplification of CD95 activation by caspase 8-induced endosomal acidification in rat hepatocytes: R. Reinehr, et al.; J. Biol. Chem. 283, 2211 (2008) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P48023: FasL (human)
AfCs Signalling Gateway link A002830: FasL (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Mature T cells of autoimmune lpr/lpr mice have a defect in antigen-stimulated suicide: J.H. Russell, et al.; PNAS 90, 4490 (1993) Abstract
Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways: B. Lowin, et al.; Nature 370, 650 (1994) Abstract
A role for CD95 ligand in preventing graft rejection: D. Bellgrau, et al.; Nature 377, 630 (1995) Abstract
Fas ligand-induced apoptosis as a mechanism of immune privilege: T.S. Griffith, et al.; Science 270, 1189 (1995) Abstract
Melanoma cell expression of Fas(Apo-1/CD95) ligand: implications for tumor immune escape: M. Hahne, et al.; Science 274, 1363 (1996) Abstract
Apoptosis by death factor: S. Nagata; Cell 88, 355 (1997) Abstract
Related Products
Further Categories Containing This Product:
Receptor / Ligand + Enhancer Set • CD Kits & Sets
 
 
ALX-522-020 Revised 16-May-06
SuperFasLigand™, Soluble (human) (recombinant)
Add to Clipboard
SYNONYMS APO-1L, Soluble (human) (recombinant)
CD95L, Soluble (human) (recombinant)
CD178, Soluble (human) (recombinant)
TNFSF 6, Soluble (human) (recombinant)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY FasL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-020-C005   5 µg 250.00 USD Add To Cart
ALX-522-020-3005 SuperPack 3x5 µg 500.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
MW: ~32kDa (nonglycosylated), ~35kDa (glycosylated).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG®-tag. Glycosylation of rhsSuperFasLigandTM is similar to natural human FasL.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with cell culture medium containing 5% fetal calf serum.
SPECIFICITY: Binds to human, mouse and rat Fas (CD95; APO-1).
BIOLOGICAL ACTIVITY: Kills Fas-sensitive cells at concentrations of >1ng/ml.
Note: Does not require enhancer.
SPECIFIC ACTIVITY: ED50: 1ng/ml (A20 cells).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Fas ligand (CD95L) protects neurons against perforin-mediated T lymphocyte cytotoxicity: I. Medana, et al.; J. Immunol. 167, 674 (2001) Abstract; Full Text
Tumor-cell resistance to death receptor-induced apoptosis through mutational inactivation of the proapoptotic Bcl-2 homolog Bax: H. LeBlanc, et al.; Nat. Med. 8, 274 (2002) Abstract
Inhibition of metalloproteinase cleavage enhances the cytotoxicity of Fas ligand: P.G. Knox, et al.; J. Immunol. 170, 677 (2003) Abstract; Full Text
NF-kappa B is required for surface Ig-induced Fas resistance in B cells: B.R. Schram & T.L. Rothstein; J. Immunol. 170, 3118 (2003) Abstract; Full Text
TNF-related apoptosis-inducing ligand (TRAIL) frequently induces apoptosis in Philadelphia chromosome-positive leukemia cells: K. Uno, et al.; Blood 101, 3658 (2003) Abstract
Fas Ligand Down-Regulates Cytokine-Induced Fas Receptor Expression on Insulinoma (NIT-1), But Not Islet Cells, from Autoimmune Nonobese Diabetic Mice: P. Augstein, et al.; Endocrinology 145, 2747 (2004) Abstract
In vitro engagement of CD3 and CD28 corrects T cell defects in chronic lymphocytic leukemia: M. Bonyhadi, et al.; J. Immunol. 174, 2366 (2005) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Note: Results using rhsSuperFasLigand™ may differ from those obtained with agonistic antibodies!

Swiss-Prot link P48023: FasL (human)
AfCs Signalling Gateway link A002830: FasL (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Mature T cells of autoimmune lpr/lpr mice have a defect in antigen-stimulated suicide: J.H. Russel, et al.; PNAS 90, 4409 (1993) Abstract
A role for CD95 ligand in preventing graft rejection: D. Bellgrau, et al.; Nature 377, 630 (1995) Abstract
Melanoma cell expression of Fas(Apo-1/CD95) ligand: implications for tumor immune escape: M. Hahne, et al.; Science 274, 1363 (1996) Abstract
Apoptosis by death factor