• Home
  • Sitemap
  • Help
  • Technical Support
  • Contact
Toll-like Receptors [TLRs]/Related Products
You are here: Product Lines > Inflammation > Toll-like Receptors [TLRs]/Related Products
 
Toolbar - View Selection
 
 Items 1-20 of 110 Page 1 of 6 Select Page: 1 2 3 4 5 6  >>  
ALX-430-064 Revised 06-Jul-07
Auranofin
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY Lipoxygenases/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-430-064-M025   25 mg 35.00 USD Add To Cart
ALX-430-064-M100   100 mg 68.00 USD Add To Cart
Product Specification
FORMULA: C20H34AuO9PS
MW: 678.5
CAS NUMBER: 34031-32-8
MERCK INDEX: 14: 878
PURITY: ≥98%
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO or 100% ethanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
USE/STABILITY: Solutions are stable for up to 3 months when stored at -20 °C.
HAZARD: TOXIC.

Product Description
Gold derivative in clinical use as an anti-arthritic. Acts as an inhibitor of several leukocyte activation pathways. Inhibits release of inflammatory mediators from human macrophages, pulmonary mast cell and basophils. Also inhibits human neutrophil 5-lipoxygenase. Efficient inducer of mitochondrial membrane permeability transition pore in the presence of calcium ions, potentially referable to its inhibition of mitochondrial thioredoxin reductase.
Product Specific Literature References
Antiarthritic gold compounds effectively quench electronically excited singlet oxygen: E.J. Corey, et al.; Science 236, 68 (1987) Abstract
Modulation of mediator release from human basophils and pulmonary mast cells and macrophages by auranofin: M. Columbo, et al.; Biochem. Pharmacol. 39, 285 (1990) Abstract
Auranofin stimulates LTA hydrolase and inhibits 5-lipoxygenase/LTA synthase activity of isolated human neutrophils: W.H. Betts, et al.; Biochem. Pharmacol. 39, 1233 (1990) Abstract
Auranofin inhibits the activation pathways of polymorphonuclear leukocytes at multiple sites: R. Rudkowski, et al.; Biochem. Pharmacol. 41, 1921 (1991) Abstract
Induction of mitochondrial permeability transition by auranofin, a Gold(I)-phosphine derivative: M.P. Rigobello, et al.; Br. J. Pharmacol. 136, 1162 (2002) Abstract
General Literature References
Auranofin: W.F. Kean, et al.; Br. J. Theumatol. 36, 560 (1997), (Review) Abstract; Full Text
Further Categories Containing This Product:
MPTP [Mitochondrial Transition Pore]/Related Products • TLRs Other Products • Anti-inflammatory Agents Other Products
 
 
ALX-201-133 Revised 19-Feb-08
CD14 (human) (recombinant)
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-133-C010   10 µg 270.00 USD Add To Cart
Product Specification
MW: ~50kDa.
SOURCE/HOST: Produced in CHO cells.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ~90-95% (SDS-PAGE)
PURITY DETAIL: Affinity purified using MAb to CD14 (human) (biG 2/RoMo-1) (Prod. No. ALX-804-498).
FORMULATION: Lyophilized from PBS, pH 7.2.
ENDOTOXIN CONTENT: <0.00015EU/µg protein (LAL test; Chromogenix).
RECONSTITUTION: Reconstitute with 10µl distilled water. Further dilutions should be made with PBS
BIOLOGICAL ACTIVITY: Inhibits binding of LPS to CD14. Up to 20µg/ml CD14 inhibit binding of FITC-LPS (0.5µg/ml) to 6x105 CD14+ CHO transfectants (FACS).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Both membrane-bound and soluble forms of CD14 bind to gram-negative bacteria: R.S. Jack, et al.; Eur. J. Immunol. 25, 1436 (1995) Abstract
The myeloid differentiation antigen CD14 is N- and O-glycosylated. Contribution of N-linked glycosylation to different soluble CD14 isoforms: F. Stelter, et al.; Eur. J. Biochem. 236, 457 (1996) Abstract
Proteolysis of monocyte CD14 by human leukocyte elastase inhibits lipopolysaccharide-mediated cell activation: K. Le-Barillec, et al.; J. Clin. Invest. 103, 1039 (1999) Abstract; Full Text
General Information
The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 ist the 50kDa protein.
The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore it is present in a soluble form in mouse serum, urine and other body fluids.
MANUFACTURER Manufactured by Biometec.
Related Products
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins • CD Proteins
 
 
ALX-201-134 Revised 20-Feb-07
CD14 (mouse) (recombinant) (His)
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-134-C010   10 µg 370.00 USD Add To Cart
Product Specification
MW: ~50kDa
SOURCE/HOST: Produced in CHO cells.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY DETAIL: His-tag-affinity purified.
FORMULATION: Lyophilized from PBS, pH 7.2.
ENDOTOXIN CONTENT: <0.00015EU/µg protein (LAL test; Chromogenix).
RECONSTITUTION: Reconstitute with 10µl distilled water.
BIOLOGICAL ACTIVITY: Inhibits binding of LPS to CD14. Up to 5µg/ml mouse CD14 inhibit binding of FITC-LPS (0.5µg/ml) to mouse CD14+ CHO transfectants (FACS).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Monocytes can phagocytose Gram-negative bacteria by a CD14-dependent mechanism: U. Grunwald, et al.; J. Immunol. 157, 4119 (1996) Abstract
General Information
The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 ist the 50kDa protein.
MANUFACTURER Manufactured by Biometec.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins • CD Proteins
 
 
ALX-850-302 Revised 16-Nov-05
CD14, Soluble (human) Detection Set [For ELISA Application]
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-302-KI01   1 Set 690.00 USD Add To Cart
Product Specification
QUANTITY: 96 wells (~80 tests).
FORMULATION: All reagents are stabilized with 0.01% thimerosal.
APPLICATION: For the quantitative detection of soluble human CD14 levels.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
General Information
MANUFACTURER Manufactured by Biometec.
Further Categories Containing This Product:
ELISA Sets
 
 
ALX-850-303 Revised 16-Nov-05
CD14, Soluble (mouse) Detection Set [For ELISA Application]
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-303-KI01   1 Set 990.00 USD Add To Cart
Product Specification
QUANTITY: 96 wells (~80 tests).
FORMULATION: All reagents are stabilized with 0.01% thimerosal.
APPLICATION: For the quantitative detection of soluble mouse CD14 levels.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
General Information
MANUFACTURER Manufactured by Biometec.
Further Categories Containing This Product:
ELISA Sets
 
 
ALX-505-008 Revised 29-May-07
ddWater (endotoxin-free)
Add to Clipboard
PRODUCT LINE Molecular Biology
PRODUCT CATEGORY Buffers/Detergents/Solutions
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-505-008-LD15   1.5 ml 45.00 USD Add To Cart
Product Specification
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
Product Description
Sterile, double distilled endotoxin-free water for use with TLRgrade™ or endotoxin-free grade reagents. Product has been subjected to multiple rounds of LPS removal by adsorption with activated charcoal [1].

Related Products:

ODN 1668 (TLRgrade™) (synthetic) (Prod. No. ALX-746-001)    
ODN 1826 (TLRgrade™) (synthetic) (Prod. No. ALX-746-002)      
ODN 1585 (TLRgrade™) (synthetic) (Prod. No. ALX-746-003)     
ODN M362 (TLRgrade™) (synthetic) (Prod. No. ALX-746-004)    
ODN 2216 (TLRgrade™) (synthetic) (Prod. No. ALX-746-005)      
ODN 2006 (TLRgrade™) (synthetic) (Prod. No. ALX-746-006)    
ODN 2395 (TLRgrade™) (synthetic) (Prod. No. ALX-746-020)     
ODN 1720 (TLRgrade™) (synthetic) (Control) (Prod. No. ALX-746-200)    
ODN 1982 (TLRgrade™) (synthetic) (Control) (Prod. No. ALX-746-201)     
ODN 2118 (TLRgrade™) (synthetic) (Control) (Prod. No. ALX-746-203)     
ODN M383 (TLRgrade™) (synthetic) (Control) (Prod. No. ALX-746-204)     
ODN 2243 (TLRgrade™) (synthetic) (Control) (Prod. No. ALX-746-205)   
ODN 2137 (TLRgrade™) (synthetic) (Control) (Prod. No. ALX-746-206)
iODN 2088 (TLRgrade™) (synthetic) (Inhibitory for mouse) (Prod. No. ALX-746-250)
iODN (ttaggg)4 (TLRgrade™) (synthetic) (Inhibitory for human) (Prod. No. ALX-746-251)
Poly(I:C) . potassium salt (TLRgrade™) (synthetic) (Prod. No. ALX-746-021)
Product Specific Literature References
The removal of 14C labeled endotoxin by activated charcoal: A.S. Pegues, et al.; Int. J. Artif. Organs 2, 153 (1979) Abstract
Related Products
Further Categories Containing This Product:
Lipopolysaccharides [LPS]/Related Products • Oligodeoxynucleotides [ODNs]
 
 
ALX-522-058 Revised 05-Jun-08
Flagellin (high purity)
Add to Clipboard
SYNONYMS FliC (high purity)
PRODUCT LINE Inflammation
PRODUCT CATEGORY TLR Agonists Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-058-C010   10 µg 298.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: Isolated from Salmonella typhimurium strain 14028.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water.
SPECIFICITY: Binds to human and mouse TLR5 (Toll-like receptor 5).
BIOLOGICAL ACTIVITY: Activation of TLR5 in human epithelial cell assays based on NF-κB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, β-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Flagellin stimulation of intestinal epithelial cells triggers CCL20-mediated migration of dendritic cells: F. Sierro, et al.; PNAS 98, 13722 (2001) Abstract; Full Text
Pathophysiological role of Toll-like receptor 5 engagement by bacterial flagellin in colonic inflammation: S.H. Rhee, et al.; PNAS 102, 13610 (2005) Abstract
General Information
Motile bacteria produce flagella, filaments composed mainly of the structural subunit flagellin. Flagellins are highly conserved molecules among both gram negative and gram positive bacteria, especially in 170 N-terminal and 100 C-terminal amino acids. In mammalian cells, Toll-like receptor 5 (TLR5) senses various flagellins resulting in activation of innate and adaptive immunity. The phase 1 flagellin FliC from Salmonella typhimurium (serotype H 'I'), a protein of 494 amino acids, upregulates production of chemokines and antimicrobial compounds in human epithelial cells and monocytes and induces maturation of dendritic cells.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P06179: Flagellin (Salmonella typhimurium)
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5: F. Hayashi, et al.; Nature 410, 1099 (2001) Abstract
Salmonella typhimurium translocates flagellin across intestinal epithelia, inducing a proinflammatory response: A.T. Gewirtz, et al.; J. Clin. Invest. 107, 99 (2001) Abstract
Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression: A.T. Gewirtz, et al.; J. Immunol. 167, 1882 (2001) Abstract
Salmonella flagellin-dependent proinflammatory responses are localized to the conserved amino and carboxyl regions of the protein: T.D. Eaves-Pyles, et al.; J. Immunol. 167, 7009 (2001) Abstract
Flagellin, a novel mediator of Salmonella-induced epithelial activation and systemic inflammation: I kappa B alpha degradation, induction of nitric oxide synthase, induction of proinflammatory mediators, and cardiovascular dysfunction: T. Eaves-Pyles, et al.; J. Immunol. 166, 1248 (2001) Abstract
Enteroaggregative Escherichia coli expresses a novel flagellin that causes IL-8 release from intestinal epithelial cells: T.S. Steiner, et al.; J. Clin. Invest. 105, 1769 (2000) Abstract
Salmonella flagellin induces tumor necrosis factor alpha in a human promonocytic cell line: F. Ciacci-Woolwine, et al.; Infect. Immun. 66, 1127 (1998) Abstract
Locations of terminal segments of flagellin in the filament structure and their roles in polymerization and polymorphism: Y. Mimori-Kiyosue, et al.; J. Mol. Biol. 270, 222 (1997) Abstract
Further Categories Containing This Product:
Natural Proteins
 
 
ALX-420-040 Revised 05-Mar-07
Gardiquimod
Add to Clipboard
SYNONYMS 1-(4-Amino-2-ethylaminomethylimidazo[4,5-c]quinolin-1-yl)-2-methylpropan-2-ol
PRODUCT LINE Inflammation
PRODUCT CATEGORY TLR Agonists Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-420-040-M025   25 mg 130.00 USD Add To Cart
ALX-420-040-M100   100 mg 390.00 USD Add To Cart
Product Specification
FORMULA: C17H23N5O
MW: 313.4
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO (>40mg/ml), dioxane (7mg/ml), methanol (>40mg/ml) or acetonitrile (2mg/ml).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Selective ligand for human or mouse Toll-like receptor 7 (TLR7). Induces the activation of NF-κB in HEK 293 cells expressing TLR7. At high concentrations (3µg/ml) slightly activates TLR8. More active than Imiquimod (Prod. No. ALX-420-039).
 
 
ALX-420-039 Revised 27-Feb-07
Imiquimod
Add to Clipboard
SYNONYMS 1-(2-Methylpropyl)-1H-imidazo[4,5-c]quinolin-4-amine
R-837
PRODUCT LINE Inflammation
PRODUCT CATEGORY TLR Agonists Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-420-039-M100   100 mg 55.00 USD Add To Cart
ALX-420-039-M250   250 mg 120.00 USD Add To Cart
Product Specification
FORMULA: C14H16N4
MW: 240.3
CAS NUMBER: 99011-02-6
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO, dimethyl formamide or water (warm to 37°C, vortex); insoluble in 100% ethanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for up to 1 year after receipt when stored at -20°C. Stock solutions are stable for up to 3 months when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C.

Product Description
Topical immune response modifier that inhibits angiogenesis. Up-regulates IL-18 and down-regulates MMP-9 through recognition of Toll-like receptor 7 (TLR7) and subsequent activation of MyD88-dependent pathway.
Product Specific Literature References
Imiquimod applied topically: a novel immune response modifier and new class of drug: R.L. Miller, et al.; Int. J. Immunopharmacol. 21, 1 (1999) Abstract
Small anti-viral compounds activate immune cells via the TLR7 MyD88-dependent signaling pathway: H. Hemmi, et al.; Nat. Immunol. 3, 196 (2002) Abstract
In vivo and in situ modulation of the expression of genes involved in metastasis and angiogenesis in a patient treated with topical imiquimod for melanoma skin metastases: C. Hesling, et al.; Br. J. Dermatol. 150, 761 (2004) Abstract
Imiquimod as an antiangiogenic agent: V.W. Li, et al.; J. Drugs Dermatol. 4, 708 (2005) Abstract
Imiquimod is a strong inhibitor of tumor cell-induced angiogenesis: S. Majewski, et al.; Int. J. Dermatol. 44, 14 (2005) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Stimulation of TLR7 can be achieved with 1-10µg/ml of the compound for 6 to 24 hours.

Related Products:
- ddWater (endotoxin-free) (Prod. No. ALX-505-008)
- PBS (endotoxin-free) (Prod. No. ALX-505-007)
Related Products
Further Categories Containing This Product:
Antitumor Agents (Apoptosis Inducers) • Angiogenesis Modulators Other Products • MMPs Other Products • Inflammasome