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ALX-480-021
Revised 16-Dec-04
Adenosine 5'-triphosphate . disodium salt
SYNONYMS
ATP . 2Na
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Adenosine Derivatives Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-480-021-G001
1 g
15.00 USD
ALX-480-021-G005
5 g
45.00 USD
Product Specification
FORMULA:
C
10
H
14
N
5
O
13
P
3
. 2Na
MW:
505.2 . 46.0
CAS NUMBER:
51963-61-2
MERCK INDEX:
14:
155
PURITY:
≥99%
APPEARANCE:
White to off-white powder.
SOLUBILITY:
Soluble in water.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from moisture. Keep cool and dry.
Further Categories Containing This Product:
Inflammasome
ALX-840-602
Revised 24-Nov-05
Positive Control (Cell Lysate) for Antibodies to Asc (human)
SYNONYMS
Positive Control (Cell Lysate) for Antibodies to Pycard (human)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
Positive Controls (Cell Lysates & Extracts)
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-840-602-R100
100 µl
70.00 USD
Product Specification
SOURCE/HOST:
Whole cell lysate of HEK 293T cells transfected with an expression plasmid for human Asc (aa 1-195) fused at the C-terminus to a FLAG
®
-tag.
QUANTITY:
100µl corresponding to 2x10
5
cells.
FORMULATION:
Liquid. In 5x sample buffer (312.5mM TRIS, pH 6.8, containing 10% SDS, 6% glycerol, 0.075% bromophenol blue, 10% β-mercaptoethanol and 10mM DTT).
APPLICATION:
Positive control in Western blot for PAb to Asc (human) (AL177) (Prod. No.
ALX-210-905
). Add 13µl sterile water and 2µl DTT (100mM) to 5µl of cell lysate.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION
FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER
Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Caspase Recruitment Domain [CARD] Proteins/Related Products
•
Inflammasome
ALX-201-056
Revised 21-Jul-08
Caspase-1 (active) (human) (recombinant)
SYNONYMS
Interleukin 1
β Converting Enzyme (active) (human) (recombinant)
IL-1β Converting Enzyme (active) (human) (recombinant)
ICE (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-056-U025
25 U
231.00 USD
ALX-201-056-U100
100 U
385.00 USD
Product Specification
EC:
3.4.22.36
SOURCE/HOST:
Produced in
E. coli
.
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
~20’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate YVAD-
p
NA (
p
NA:
p
-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
QUALITY CONTROL:
Routinely tested for its ability to cleave the caspase-1 colorimetric substrate Ac-YVAD-
p
NA (Prod. No.
ALX-260-026
).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
Caspase-1 is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20kDa) and small (10kDa) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form.
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P29466:
Caspase-1 (human) (precursor)
AfCS Signalling Gateway link A000492:
Caspase-1 (mouse)
Further Categories Containing This Product:
Enzymes
•
Inflammasome
•
Recombinant Proteins/Fusion Proteins
ALX-850-211
Revised 22-Apr-08
Caspase-1 Colorimetric Assay Kit
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Kits & Sets
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-850-211-KI01
25 tests
175.00 USD
Product Specification
KIT/SET CONTAINS:
25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl YVAD-
p
NA (1mM), 100µl DTT (1M), 25ml Dilution Buffer.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from light. Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening.
Product Description
Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate YVAD-pNA.
General Information
The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.
MANUFACTURER
Manufactured by BioVision.
Further Categories Containing This Product:
Inflammasome
•
Activity Assays
ALX-850-212
Revised 21-Apr-08
Caspase-1 Fluorometric Assay Kit
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Kits & Sets
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-850-212-KI01
25 tests
175.00 USD
Product Specification
KIT/SET CONTAINS:
25ml Cell Lysis Buffer, 2ml 2x Reaction Buffer, 125µl YVAD-AFC Substrate (1mM), 100µl DTT (1M).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from light. Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening.
Product Description
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate YVAD-AFC.
General Information
YVAD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.
MANUFACTURER
Manufactured by BioVision.
Further Categories Containing This Product:
Inflammasome
•
Activity Assays
ALX-201-094
Revised 03-May-06
Caspase-5 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-094-U025
25 U
203.00 USD
ALX-201-094-U100
100 U
371.00 USD
Product Specification
EC:
3.4.22.-
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
~5'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
In combination with caspase-5 activity assays, the enzyme is useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase-5 activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P51878:
Caspase-5 (human) (precursor)
AfCS Signalling Gateway link A000500:
Caspase-5 (mouse)
Further Categories Containing This Product:
Enzymes
•
Inflammasome
•
Recombinant Proteins/Fusion Proteins
ALX-850-217
Revised 24-Jan-05
Caspase-5 Colorimetric Assay Kit
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Kits & Sets
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-850-217-KI01
25 tests
203.00 USD
Product Specification
KIT/SET CONTAINS:
25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl WEHD-pNA Substrate (4mM), 100µl DTT (1M), 25ml Dilution Buffer.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening.
Product Description
Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate WEHD-pNA.
General Information
The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.
MANUFACTURER
Manufactured by BioVision.
Further Categories Containing This Product:
Inflammasome
•
Activity Assays
ALX-850-218
Revised 24-Jan-05
Caspase-5 Fluorometric Assay Kit
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Kits & Sets
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-850-218-KI01
25 tests
189.00 USD
Product Specification
KIT/SET CONTAINS:
25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl WEHD-AFC Substrate (1mM), 100µl DTT (1M).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening.
Product Description
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate WEHD-AFC.
General Information
WEHD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-5 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.
MANUFACTURER
Manufactured by BioVision.
Further Categories Containing This Product:
Inflammasome
•
Activity Assays
ALX-420-039
Revised 14-Aug-08
Imiquimod
SYNONYMS
1-(2-Methylpropyl)-1H-imidazo[4,5-c]quinolin-4-amine
R-837
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
TLR Agonists Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-420-039-M100
100 mg
55.00 USD
ALX-420-039-M250
250 mg
120.00 USD
Product Specification
FORMULA:
C
14
H
16
N
4
MW:
240.3
CAS NUMBER:
99011-02-6
APPEARANCE:
White to off-white powder.
SOLUBILITY:
Soluble in DMSO (3mg/ml) or dimethyl formamide; slightly soluble in water (warm to 37°C, vortex); insoluble in 100% ethanol.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for up to 1 year after receipt when stored at -20°C. Stock solutions are stable for up to 3 months when stored at -20°C.
HANDLING:
After reconstitution, prepare aliquots and store at -20°C.
Product Description
Topical immune response modifier that inhibits angiogenesis. Up-regulates IL-18 and down-regulates MMP-9 through recognition of Toll-like receptor 7 (TLR7) and subsequent activation of MyD88-dependent pathway.
Product Specific Literature References
Imiquimod applied topically: a novel immune response modifier and new class of drug:
R.L. Miller, et al.; Int. J. Immunopharmacol.
21
, 1 (1999)
Abstract
Small anti-viral compounds activate immune cells via the TLR7 MyD88-dependent signaling pathway:
H. Hemmi, et al.; Nat. Immunol.
3
, 196 (2002)
Abstract
In vivo and in situ modulation of the expression of genes involved in metastasis and angiogenesis in a patient treated with topical imiquimod for melanoma skin metastases:
C. Hesling, et al.; Br. J. Dermatol.
150
, 761 (2004)
Abstract
Imiquimod as an antiangiogenic agent:
V.W. Li, et al.; J. Drugs Dermatol.
4
, 708 (2005)
Abstract
Imiquimod is a strong inhibitor of tumor cell-induced angiogenesis:
S. Majewski, et al.; Int. J. Dermatol.
44
, 14 (2005)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Stimulation of TLR7 can be achieved with 1-10µg/ml of the compound for 6 to 24 hours.
Related Products:
- ddWater (endotoxin-free) (Prod. No.
ALX-505-008
)
- PBS (endotoxin-free) (Prod. No.
ALX-505-007
)
Related Products
ALX-505-007
PBS (endotoxin-free)
ALX-505-008
ddWater (endotoxin-free)
Further Categories Containing This Product:
Antitumor Agents (Apoptosis Inducers)
•
Angiogenesis Modulators Other Products
•
MMPs Other Products
•
Inflammasome
ALX-804-530
Revised 14-Aug-07
Monoclonal Antibody to Caspase-1 (mouse) (1H11)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-530-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
1H11
ISOTYPE:
Rat IgG1
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. Protein G sepharose purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN:
p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T
206
ALEMVKEVKEFAAC
220
) of mouse caspase-1.
SPECIFICITY:
Recognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45kDa by Western blot. Does not cross-react with mouse caspase-11 or -12.
APPLICATION:
ELISA
Flow Cytometry
Western Blot
Other applications not tested.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death:
V.S. Marsden, et al.; Blood
107
, 1872 (2006)
Abstract
;
Full Text
General Information
Capases are a highly conserved family of cysteine proteases and are synthesized as inactive zymogens, which are cleaved at Asp-x sites during apoptosis, generating large and small subunits that heterodimerize to form the active enzyme. Caspase-1 is a member of the ICE/CED-3 family of caspases and is detected in the pro-form as a protein of 45 kDa.
Caspase-1 generates the bioactive form of the pro-inflammatory cytokine IL-1β from its biologically inactive precursor. Overexpression of caspase-1 in cells induces apoptosis, which can be inhibited by cytokine response modifier A (CrmA). Caspase-1 mRNA is predominantly expressed in the spleen and lung and to a lesser extent in skeletal muscle, kidney, testis and heart and within these tissues macrophages preferentially express caspase-1. Caspase-1 deficient mice have no gross abnormalities, however, macrophages from caspase-1-deficient mice are defective in IL-β processing and release.
General Literature References
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins
:
L.A. O'Reilly, et al.; Biotechniques
25
, 824 (1998), Methodology
Abstract
Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9
:
L.A. O'Reilly, et al.; Cell Death Differ.
9
, 832 (2002), Methodology
Abstract
Further Categories Containing This Product:
Inflammasome
•
Monoclonal Antibodies
ALX-804-531
Revised 10-Mar-06
Monoclonal Antibody to Caspase-1 (mouse) (4G8)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-531-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
4G8
ISOTYPE:
Rat IgG1
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. Protein G sepharose purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN:
p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T
206
ALEMVKEVKEFAAC
220
) of mouse caspase-1.
SPECIFICITY:
Recognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45kDa by Western blot. Does not cross-react with mouse caspase-11 or -12.
APPLICATION:
ELISA
Flow Cytometry
Western Blot
Other applications not tes
