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Inflammation
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ALX-581-019 Revised 10-Sep-08
LPS from Salmonella enteritidis S-Form (TLRgrade™) (Ready-to-Use)
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SYNONYMS Lipopolysaccharide from Salmonella enteritidis S-Form (TLRgrade™) (Ready-to-Use)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Lipopolysaccharides [LPS] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-581-019-L002   2 ml 155.00 USD Add To Cart
Product Specification
SOURCE/HOST: Smooth (S)-form LPS isolated and purified from Salmonella enteritidis (wild type) by a modification of the PCP extraction method [1,2], converted to the uniform sodium salt form [3] and dissolved in sterile pyrogen-free double distilled water.
CONCENTRATION: 1mg/ml
PURITY: Absence of detectable protein or DNA contaminants with agonistic TLR activity.
FORMULATION: Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
ACTIVITY: Specific activator of Toll-like receptor 4 (TLR4). Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice.
Smooth (S)-form LPS are commonly the preferred choice for whole animal studies, whereas Rough (R)-form LPS are primarily used in cellular in vitro activation studies.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at +4°C.
HANDLING: Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Avoid inhalation of LPS and prevent these compounds from entering the bloodstream. Keep sterile. Use must be restricted to qualified personnel.
HAZARD: HIGHLY PYROGENIC. HARMFUL.
Product Specific Literature References
[1] A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969) Abstract
[2] Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Luderitz; Meth. Carbohydr. Chem. 9, 11 (1993)
[3] Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Luderitz; Eur. J. Biochem. 54, 603 (1975) Abstract
 
 
ALX-581-020 Revised 10-Sep-08
LPS from Salmonella minnesota S-Form (TLRgrade™) (Ready-to-Use)
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SYNONYMS Lipopolysaccharide from Salmonella minnesota S-Form (TLRgrade™) (Ready-to-Use)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Lipopolysaccharides [LPS] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-581-020-L002   2 ml 155.00 USD Add To Cart
Product Specification
SOURCE/HOST: Smooth (S)-form LPS isolated and purified from Salmonella minnesota (wild type) by a modification of the PCP extraction method [1,2], converted to the uniform sodium salt form [3] and dissolved in sterile pyrogen-free double distilled water.
CONCENTRATION: 1mg/ml
PURITY: Absence of detectable protein or DNA contaminants with agonistic TLR activity.
FORMULATION: Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
ACTIVITY: Specific activator of Toll-like receptor 4 (TLR4). Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice.
Smooth (S)-form LPS are commonly the preferred choice for whole animal studies, whereas Rough (R)-form LPS are primarily used in cellular in vitro activation studies.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at +4°C.
HANDLING: Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Avoid inhalation of LPS and prevent these compounds from entering the bloodstream. Keep sterile. Use must be restricted to qualified personnel.
HAZARD: HIGHLY PYROGENIC. HARMFUL.
Product Specific Literature References
[1] A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969) Abstract
[2] Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Luderitz; Meth. Carbohydr. Chem. 9, 11 (1993)
[3] Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Luderitz; Eur. J. Biochem. 54, 603 (1975) Abstract
 
 
ALX-581-150 Revised 16-Sep-08 New product
LPS from Salmonella abortus equi S-form (TLRgrade™) (Ready-to-Use) (Biotin)
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SYNONYMS Lipopolysaccharide from Salmonella abortus equi S-form (TLRgrade™) (Ready-to-Use) (Biotin)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Lipopolysaccharides [LPS] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-581-150-R500   500 µl 395.00 USD Add To Cart
Product Specification
SOURCE/HOST: Smooth (S)-form LPS, isolated and purified from Salmonella abortus equi by modification of the phenol water extraction and PCP method [1]. LPS-Biotin was prepared using the biotin reagent biotinamidocaproate N-hydroxysuccinimide ester. Briefly, LPS at 10mg/ml in distilled water was mixed with biotin reagent in sodium bicarbonate buffer. The reaction mixture was stirred, dialyzed extensively against distilled water in the dark, and sterile filtered.
CONCENTRATION: 1mg/ml
PURITY: Absence of dectectable protein or DNA contaminants with agonistic TLR activity.
FORMULATION: Liquid. Sterile, ready-to-use solution in pyrogen-free double-distilled water.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: After opening, prepare aliquots and store at -20°C. Use must be restricted to qualified personnel. Keep sterile. Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Avoid inhalation of LPS and prevent these compounds from entering the bloodstream.
HAZARD: HIGHLY PYROGENIC. HARMFUL.
Product Description
Useful reagent for evaluating LPS binding and cell activation in white blood cells.
Product Specific Literature References
[1] Newer aspects of the chemsitry and biology of bacterial lipopolysaccharides, with special reference to their lipid A component: C. Galanos, et al.; Int. Rev. Biochem. 14, 239 (1977)
[2] Lipopolysaccharide-cell interaction and induced cellular activation in whole blood of septic patients: R. Salomao, et al.; J. Endotoxin Res. 8, 371 (2002) Abstract
[3] Influence of EDTA and heparin on lipopolysaccharide binding and cell activation, evaluated at single-cell level in whole blood: M.K. Brunialti, et al.; Cytometry 50, 14 (2002) Abstract; Full Text
[4] Peripheral blood mononuclear cell activation induced by Leptospira interrogans glycolipoprotein: D. Diament, et al.; Infect. Immun. 70, 1677 (2002) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Note: As the biotinylation reagent and standard biotinylation protocol favors protein/peptide (contaminants) about 1000-fold over sugar as a target, it is mandatory to use >99.9% pure LPS to exclude the biotinylation of contaminants. These are usually present in many commercial LPS preparations and would lead to misleading observations of the tracking/staining with the reagents.
TLRgrade™ LPS fulfills such high quality requirements and the biotinylation procedure is chosen to preserve TLR4 (Toll-like receptor 4) binding and cellular activation using TLRgrade™ LPS-Biotin.

Related Product:

- For the unconjugated LPS, please see LPS from Salmonella abortus equi S-form (TLRgrade™) (Ready-to-Use) (Prod. No. ALX-581-009).
Related Products
Further Categories Containing This Product:
Biotinylated Reagents Other Products
 
 
ALX-581-200 Revised 16-Sep-08
Lipid A from E. coli, Serotype R515 (Re) (TLRgrade™) (Ready-to-Use)
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PRODUCT LINE Inflammation
PRODUCT CATEGORY Lipopolysaccharides [LPS] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-581-200-L002   2 ml 320.00 USD Add To Cart
Product Specification
SOURCE/HOST: Isolated from E. coli R515 (Re mutant). Prepared by mild acid hydrolysis from purified Escherichia coli R515 (Re) LPS (isolated by a modification of the PCP extraction method [1,2]), converted to the uniform salt form [3] and dissolved in sterile pyrogen-free double distilled water.
CONCENTRATION: 1mg/ml
PURITY: ≥99.9% (regarding protein or DNA contaminants; KDO not detectable)
FORMULATION: Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
ACTIVITY: Activates Toll-like receptor (TLR) 4. Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Keep sterile.
HAZARD: HIGHLY PYROGENIC. HARMFUL.
Product Images
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Product Specific Literature References
[1] A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969) Abstract
[2] Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Luderitz; Methods in Carbohydrate Chem. 9, 11 (1993)
[3] Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Luderitz; Eur. J. Biochem. 54, 603 (1975) Abstract
[4] R-form LPS, the master key to the activation ofTLR4/MD-2-positive cells: M. Huber, et al.; Eur. J. Immunol. 36, 701 (2006) Abstract
 
 
ALX-581-201 Revised 16-Sep-08
Lipid A from Salmonella minnesota R595 (Re) (TLRgrade™) (Ready-to-Use)
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PRODUCT LINE Inflammation
PRODUCT CATEGORY Lipopolysaccharides [LPS] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-581-201-L002   2 ml 320.00 USD Add To Cart
Product Specification
SOURCE/HOST: Isolated from Salmonella minnesota R595 (Re mutant). Prepared by mild acid hydrolysis from purified Salmonella minnesota R595 (Re) LPS (isolated by a modification of the PCP extraction method [1,2]), converted to the uniform salt form [3] and dissolved in sterile pyrogen-free double distilled water.
CONCENTRATION: 1mg/ml
PURITY: ≥99.9% (regarding protein or DNA contaminants; KDO not detectable)
FORMULATION: Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
ACTIVITY: Activates Toll-like receptor (TLR) 4. Does not activate TLR2 as determined with splenocytes and macrophages from TLR4 deficient mice.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Keep sterile.
HAZARD: HIGHLY PYROGENIC. HARMFUL.
Product Specific Literature References
[1] A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969) Abstract
[2] Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Luderitz; Methods in Carbohydrate Chem. 9, 11 (1993)
[3] Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Luderitz; Eur. J. Biochem. 54, 603 (1975) Abstract
[4] CD14 is required for MyD88-independent LPS signaling: Z. Jiang, et al.; Nat. Immunol. 6, 565 (2005) Abstract
[5] IL-12 family members: differential kinetics of their TLR4-mediated induction by Salmonella Enteritidis and the impact of IL-10 in bone marrow-derived macrophages: N. Schuetze, et al.; Int. Immunol. 17, 649 (2005) Abstract
 
 
ALX-581-202 Revised 16-Sep-08
Monophosphoryl Lipid A from Salmonella minnesota R595 (Re) (TLRgrade™) (Ready-to-Use)
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SYNONYMS MPL-A from Salmonella minnesota R595 (Re) (TLRgrade™) (Ready-to-Use)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Lipopolysaccharides [LPS] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-581-202-L001   1 ml 320.00 USD Add To Cart
Product Specification
SOURCE/HOST: Isolated from Salmonella minnesota R595 (Re mutant). Prepared by acid hydrolysis from purified Salmonella minnesota R595 (Re) LPS (isolated by a modification of the PCP extraction method [1,2]), converted to the uniform salt form [3] and dissolved in sterile pyrogen-free double distilled water.
CONCENTRATION: 1mg/ml
PURITY: ≥99.9% (regarding protein or DNA contaminants; KDO not detectable)
FORMULATION: Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
ACTIVITY: Activates Toll-like receptor (TLR) 4. Does not activate TLR2 as determined with splenocytes and macrophages from TLR4 deficient mice. Essentially lacks the endotoxic and pyrogenic activity of lipid A.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Keep sterile.
Product Specific Literature References
[1] A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969) Abstract
[2] Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Luderitz; Meth. Carbohydr. Chem. 9, 11 (1993)
[3] Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Luderitz; Eur. J. Biochem. 54, 603 (1975) Abstract
 
 
ALX-581-203 Revised 16-Sep-08 New product
Monophosphoryl Lipid A from E. coli, Serotype R515 (Re) (TLRgrade™) (Ready-to-Use)
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SYNONYMS MPL-A from E. coli, Serotype R515 (Re) (TLRgrade™) (Ready-to-Use)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Lipopolysaccharides [LPS] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-581-203-L001   1 ml 395.00 USD Add To Cart
Product Specification
SOURCE/HOST: Isolated from E. coli, Serotype R515 (Re mutant). Prepared by acid hydrolysis from purified E. coli, Serotype R515 (Re) LPS (isolated by a modification of the PCP extraction method [1,2]), converted to the uniform salt form [3] and dissolved in sterile pyrogen-free double distilled water.
CONCENTRATION: 1mg/ml
PURITY: ≥99.9% (regarding protein or DNA contaminants; KDO not detectable)
FORMULATION: Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
ACTIVITY: Activates TLR4 (Toll-like receptor 4). Does not activate TLR2 as determined with splenocytes and macrophages from TLR4 deficient mice. Essentially lacks the endotoxic and pyrogenic activity of lipid A.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Keep sterile.
Product Specific Literature References
[1] A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969) Abstract
[2] Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Luderitz; Meth. Carbohydr. Chem. 9, 11 (1993)
[3] Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Luderitz; Eur. J. Biochem. 54, 603 (1975) Abstract
 
 
ALX-746-001 Revised 16-Sep-08
ODN 1668 (TLRgrade™) (synthetic)
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PRODUCT LINE Inflammation
PRODUCT CATEGORY Oligodeoxynucleotides [ODNs]
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-746-001-T100   100 tests 155.00 USD Add To Cart
Product Specification
SEQUENCE: 5’-tccatgacgttcctgatgct-3’
(lower case letters indicate phosphorothioate linkage).
MW: 6383 (ammonium salt)
SOURCE/HOST: Synthetic.
QUANTITY: 15nmol (~96µg). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate).
PURITY DETAIL: Activity and endotoxin tested - TLRgrade™.
FORMULATION: Lyophilized. Sterile.
ENDOTOXIN CONTENT: <0.02EU/µg (LAL test; BioWhittaker)
RECONSTITUTION: For a 100µM stock solution, dissolve the total vial content in 150µl endotoxin-free water (included) or PBS (to order separately).
To obtain optimal dissolving we recommend the following procedure:
- Add 50% of the solvent and let dissolve for 10 min.
- Add remaining 50% of the solvent and mix thoroughly.
- Moderate warming may aid dissolving.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
USE/STABILITY: Aqueous stock solution is stable for 1 day when stored at +4°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. For maximum product recovery after thawing, centrifuge the vial before opening the cap. Protect from light.
Product Images
Please click on thumbnails to enlarge.
Product Description
CpG oligodeoxynucleotide (Type B) with phosphorothioate backbone. Specific ligand for mouse TLR9 (Toll-like receptor 9).
General Information
BACKGROUND/TECHNICAL INFORMATION

Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008).

Related Products:

- ODN 1668 (TLRgrade™) (synthetic) (BULK) (Prod. No. ALX-746-051)

- PBS (endotoxin-free) (Prod. No. ALX-505-007)

- ODN 1720 (TLRgrade™) (synthetic) (Control) (Prod. No. ALX-746-200)

- iODN 2088 (TLRgrade™) (synthetic) (Inhibitory for mouse) (Prod. No. ALX-746-250)
Related Products
 
 
ALX-746-002 Revised 16-Sep-08
ODN 1826 (TLRgrade™) (synthetic)
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SYNONYMS CpG-B (TLRgrade™) (synthetic)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Oligodeoxynucleotides [ODNs]
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-746-002-T100   100 tests 155.00 USD Add To Cart
Product Specification
SEQUENCE: 5’-tccatgacgttcctgacgtt-3’
(lower case letters indicate phosphorothioate linkage).
MW: 6383 (ammonium salt)
SOURCE/HOST: Synthetic.
QUANTITY: 15nmol (~96µg). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate).
PURITY DETAIL: Activity and endotoxin tested - TLRgrade™.
FORMULATION: Lyophilized. Sterile.
ENDOTOXIN CONTENT: <0.02EU/µg (LAL test; BioWhittaker)
RECONSTITUTION: For a 100µM stock solution, dissolve the total vial content in 150µl endotoxin-free water (included) or PBS (to order separately).
To obtain optimal dissolving we recommend the following procedure:
- Add 50% of the solvent and let dissolve for 10 min.
- Add remaining 50% of the solvent and mix thoroughly.
- Moderate warming may aid dissolving.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
USE/STABILITY: Aqueous stock solution is stable for 1 day when stored at +4°C.
Product Images
Please click on thumbnails to enlarge.
Product Description
CpG oligodeoxynucleotide (Type B) with phosphorothioate backbone. Specific ligand for mouse TLR9 (Toll-like receptor 9). For inactive control compound see ODN 1982 (Prod. No. ALX-746-201).
General Information
BACKGROUND/TECHNICAL INFORMATION

Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008).

Related Products:

- ODN 1826 (TLRgrade™) (synthetic) (BULK) (Prod. No. ALX-746-052)

- PBS (endotoxin-free) (Prod. No. ALX-505-007)

- ODN 1982 (TLRgrade™) (synthetic) (Control) (Prod. No. ALX-746-201)

- iODN 2088 (TLRgrade™) (synthetic) (Inhibitory for mouse) (Prod. No. ALX-746-250)
Related Products
 
 
ALX-746-003 Revised 16-Sep-08
ODN 1585 (TLRgrade™) (synthetic)
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PRODUCT LINE Inflammation
PRODUCT CATEGORY Oligodeoxynucleotides [ODNs]
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-746-003-T100   100 tests 155.00 USD Add To Cart
Product Specification
SEQUENCE: 5’-ggGGTCAACGTTGAgggggg-3’
(lower case letters indicate phosphorothioate linkage).
MW: 6643 (ammonium salt)
SOURCE/HOST: Synthetic.
QUANTITY: 14.9nmol (~99µg). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate).
PURITY DETAIL: Activity and endotoxin tested - TLRgrade™.
FORMULATION: Lyophilized. Sterile.
ENDOTOXIN CONTENT: <0.02EU/µg (LAL test; BioWhittaker)
RECONSTITUTION: For a 100µM stock solution, dissolve the total vial content in 14