• Home
  • Sitemap
  • Help
  • Technical Support
  • Contact
CD14 / Related Products
You are here: Product Lines > Inflammation > CD14 / Related Products
Toolbar - View Selection
 
Items 9 of 9
ALX-201-133 Revised 19-Feb-08
CD14 (human) (recombinant)
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-133-C010   10 µg 270.00 USD Add To Cart
Product Specification
MW: ~50kDa.
SOURCE/HOST: Produced in CHO cells.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ~90-95% (SDS-PAGE)
PURITY DETAIL: Affinity purified using MAb to CD14 (human) (biG 2/RoMo-1) (Prod. No. ALX-804-498).
FORMULATION: Lyophilized from PBS, pH 7.2.
ENDOTOXIN CONTENT: <0.00015EU/µg protein (LAL test; Chromogenix).
RECONSTITUTION: Reconstitute with 10µl distilled water. Further dilutions should be made with PBS
BIOLOGICAL ACTIVITY: Inhibits binding of LPS to CD14. Up to 20µg/ml CD14 inhibit binding of FITC-LPS (0.5µg/ml) to 6x105 CD14+ CHO transfectants (FACS).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Both membrane-bound and soluble forms of CD14 bind to gram-negative bacteria: R.S. Jack, et al.; Eur. J. Immunol. 25, 1436 (1995) Abstract
The myeloid differentiation antigen CD14 is N- and O-glycosylated. Contribution of N-linked glycosylation to different soluble CD14 isoforms: F. Stelter, et al.; Eur. J. Biochem. 236, 457 (1996) Abstract
Proteolysis of monocyte CD14 by human leukocyte elastase inhibits lipopolysaccharide-mediated cell activation: K. Le-Barillec, et al.; J. Clin. Invest. 103, 1039 (1999) Abstract; Full Text
General Information
The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 ist the 50kDa protein.
The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore it is present in a soluble form in mouse serum, urine and other body fluids.
MANUFACTURER Manufactured by Biometec.
Related Products
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-134 Revised 25-Sep-08
CD14 (mouse) (recombinant) (His)
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-134-C010   10 µg 370.00 USD Add To Cart
Product Specification
MW: ~45kDa
SOURCE/HOST: Produced in CHO cells.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY DETAIL: His-tag-affinity purified.
FORMULATION: Lyophilized from PBS, pH 7.2.
ENDOTOXIN CONTENT: <0.00015EU/µg protein (LAL test; Chromogenix).
RECONSTITUTION: Reconstitute with 10µl distilled water.
BIOLOGICAL ACTIVITY: Inhibits binding of LPS to CD14. Up to 5µg/ml mouse CD14 inhibit binding of FITC-LPS (0.5µg/ml) to mouse CD14+ CHO transfectants (FACS).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Monocytes can phagocytose Gram-negative bacteria by a CD14-dependent mechanism: U. Grunwald, et al.; J. Immunol. 157, 4119 (1996) Abstract
General Information
The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 ist the 50kDa protein.
MANUFACTURER Manufactured by Biometec.
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-850-302 Revised 16-Nov-05
CD14, Soluble (human) Detection Set [For ELISA Application]
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-302-KI01   1 Set 690.00 USD Add To Cart
Product Specification
QUANTITY: 96 wells (~80 tests).
FORMULATION: All reagents are stabilized with 0.01% thimerosal.
APPLICATION: For the quantitative detection of soluble human CD14 levels.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
General Information
MANUFACTURER Manufactured by Biometec.
Further Categories Containing This Product:
ELISA Sets
 
 
ALX-850-303 Revised 16-Nov-05
CD14, Soluble (mouse) Detection Set [For ELISA Application]
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-303-KI01   1 Set 990.00 USD Add To Cart
Product Specification
QUANTITY: 96 wells (~80 tests).
FORMULATION: All reagents are stabilized with 0.01% thimerosal.
APPLICATION: For the quantitative detection of soluble mouse CD14 levels.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
General Information
MANUFACTURER Manufactured by Biometec.
Further Categories Containing This Product:
ELISA Sets
 
 
ALX-804-552 Revised 29-Jan-08
Monoclonal Antibody to CD14 (mouse) (biG 57)
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-552-C100   100 µg 400.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: biG 57
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml after reconstitution.
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Lyophilized. Contains no preservatives.
RECONSTITUTION: Reconstitute with 100µl distilled water.
IMMUNOGEN: Recombinant mouse CD14.
SPECIFICITY: Recognizes mouse CD14.
APPLICATION: ELISA
Flow Cytometry
Note: Does not inhibit binding of LPS to CD14.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
General Information
The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in mouse serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.
BACKGROUND/TECHNICAL INFORMATION Binding titer at mouse CD14 transfected CHO-cells is more than 20’000.
Positive control: The antibody binds to mouse macrophage like cell line J774 (50%) and to native mouse CD14 in mouse EDTA-blood after 6 hours of stimulation with LPS (70%).
MANUFACTURER Manufactured by Biometec.
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-804-496 Revised 02-Jun-08
Monoclonal Antibody to CD14 (biG 10)
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-496-C100   100 µg 295.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Dog
Pig
Others
CLONE: biG 10
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml after reconstitution.
PURITY DETAIL: Protein G affinity-purified.
FORMULATION: Lyophilized from PBS. Contains no preservatives.
RECONSTITUTION: Reconstitute with 100µl distilled water.
IMMUNOGEN: Monocytes and immunoaffinity purified soluble human CD14.
SPECIFICITY: Recognizes human (epitope aa 9-13 & aa 39-44), pig, dog, horse and bovine CD14.
APPLICATION: ELISA
Flow Cytometry
Western Blot
Functional Application (Inhibits binding of LPS to CD14; total inhibition at 100µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli: F. Stelter, et al.; Eur J Biochem 243, 100 (1997) Abstract
General Information
The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.
BACKGROUND/TECHNICAL INFORMATION Binding titer at human CD14 transfected CHO-cells is more than 10'000.
MANUFACTURER Manufactured by Biometec.
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-804-497 Revised 08-Feb-06
Monoclonal Antibody to CD14 (biG 14)
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-497-C100   100 µg 295.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Dog
Pig
Others
CLONE: biG 14
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml after reconstitution.
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Lyophilized from PBS. Contains no preservatives.
RECONSTITUTION: Reconstitute with 100µl distilled water.
IMMUNOGEN: Monocytes of different species and immunoaffinity purified soluble human CD14.
SPECIFICITY: Recognizes human (epitope aa 39-44), bovine, pig, dog, horse and guinea pig CD14.
APPLICATION: ELISA
Flow Cytometry
Western Blot
Functional Application (Inhibits binding of LPS to CD14; 1µg/ml MAb inhibits binding of 0.5µg/ml LPS to CD14).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli: F. Stelter, et al.; Eur J Biochem 243, 100 (1997) Abstract
General Information
The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.
BACKGROUND/TECHNICAL INFORMATION Binding titer at human CD14 transfected CHO-cells is more than 10'000.
MANUFACTURER Manufactured by Biometec.
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-804-498 Revised 18-Jul-07
Monoclonal Antibody to CD14 (human) (biG 2/RoMo-1)
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-498-C100   100 µg 295.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: biG 2/RoMo-1
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml after reconstitution.
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Lyophilized from PBS. Contains no preservatives.
RECONSTITUTION: Reconstitute with 100µl distilled water.
IMMUNOGEN: Human monocytes.
SPECIFICITY: Recognizes human (epitope aa 147-152) CD14.
APPLICATION: ELISA
Flow Cytometry
Immunoprecipitation
Note: Does not inhibit binding of LPS to CD14.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli: F. Stelter, et al.; Eur J Biochem 243, 100 (1997) Abstract
General Information
The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.
BACKGROUND/TECHNICAL INFORMATION Binding titer at human CD14 transfected CHO-cells (FACS) is 1:5000.
MANUFACTURER Manufactured by Biometec.
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-804-499 Revised 08-Apr-08
Monoclonal Antibody to CD14 (mouse) (biG 53)
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-499-C100   100 µg 490.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: biG 53
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml after reconstitution.
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Lyophilized. Contains no preservatives.
RECONSTITUTION: Reconstitute with 100µl distilled water.
IMMUNOGEN: Recombinant mouse CD14.
SPECIFICITY: Recognizes mouse CD14. Binds to LPS-activated mouse monocytes.
APPLICATION: ELISA
Flow Cytometry
Functional Application (Inhibits binding of LPS to CD14; up to 1µg/ml CD14 inhibits binding of 0.5µg/ml FITC-LPS to 6x105 CD14+ CHO transfectants (FACS)).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in mouse serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.
BACKGROUND/TECHNICAL INFORMATION Binding titer at mouse CD14 transfected CHO-cells is more than 20'000.
MANUFACTURER Manufactured by Biometec.
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
© 2008 ALEXIS Corporation, Privacy Policy, Disclaimer, Feedbackanother CS2 website

Catalogs / Brochures

E-Newsletter

 

Shopping Cart [0]

 

Distributors

Online Product Ordering

Clipboard [0]

Your items will be kept only for the duration of your visit.