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Toll-IL-1 Receptor Adaptors & TLR Downstream Signalling
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ALX-804-361 Revised 09-May-07
Monoclonal Antibody to NF-κB (p65) (112A1021)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Transcription Factors / Related Products
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ALX-804-361-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 112A1021
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 526-539 of human NF-κB (p65 subunit) .
SPECIFICITY: Recognizes human and mouse NF-κB (p65 subunit ). Detects a band of ~65kDa by Western blot.
APPLICATION: Flow Cyctometry (2.5µg/ml) (see [1])
Western Blot (2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
Product Specific Literature References
[1] Human keratinocytes respond to interleukin-18: implication for the course of chronic inflammatory skin diseases: M. Wittmann, et al.; J. Invest. Dermatol. 124, 1225 (2005) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-849 Revised 16-Aug-07
Monoclonal Antibody to RIG-I (Alme-1)
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SYNONYMS anti-RIG-1 MAb (Alme-1)
anti-Retinoic Acid-inducible Gene 1 Protein MAb (Alme-1)
anti-DEAD-box Protein 58 MAb (Alme-1)
anti-Probable ATP-dependent RNA Helicase DDX58 MAb (Alme-1)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
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ALX-804-849-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Alme-1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIG-I (retinoic acid-inducible gene 1 protein) (aa 201-713).
SPECIFICITY: Recognizes human and mouse RIG-I.
APPLICATION: Immunohistochemistry (paraffin sections)
Immunoprecipitation (1:100)
Western blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
RIG-I (retinoic acid-inducible gene I; Ddx58), a member of the DEAD-box RNA helicases, is activated by cytoplasmic dsRNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Virology. Sensing viral RNA amid your own: T. Fujita; Science 314, 935 (2006) Abstract
5’-Triphosphate RNA is the ligand for RIG-I: V. Hornung, et al.; Science 314, 994 (2006) Abstract
RIG-I-mediated antiviral responses to single-stranded RNA bearing 5’-phosphates: A. Pichlmair, et al.; Science 314, 997 (2006) Abstract
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Further Categories Containing This Product:
Antiviral Signalling / Related Products • NF-kB Pathway Other Products • Interferons & Interferon Receptors Other Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Polyclonal Antibodies
 
 
ALX-804-139 Revised 09-May-07
Monoclonal Antibody to RIP2 (human) (Nick-1)
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SYNONYMS anti-Receptor-interacting Protein 2 (human) MAb (Nick-1)
anti-CARDIAK (human) MAb (Nick-1)
anti-CARD-containing Interleukin-1β Converting Enzyme Associated Kinase (human) MAb (Nick-1)
anti-RICK (human) MAb (Nick-1)
anti-RIPK2 (human) MAb (Nick-1)
anti-Receptor-interacting Serine/Threonine Protein Kinase 2 (human) MAb (Nick-1)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY RIP Kinases
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ALX-804-139-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Nick-1
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIP2 (receptor-interacting protein 2) (aa 1-322).
SPECIFICITY: Recognizes human RIP2.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-604.
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Product Specific Literature References
Participation of Rip2 in LPS signaling is independent of its kinase activity: C. Lu, et al.; J. Biol. Chem. 280, 16278 (2005) Abstract; Full Text
PIDD mediates NF-kB activation in Response to DNA damage: Janssens, et al.; Cell 123, 1079 (2005) Abstract
Participation of Rip2 in lipopolysaccharide signaling is independent of its kinase activity: C. Lu, et al.; J. Biol. Chem. 280, 16278 (2005) Abstract; Full Text
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Nod-complex / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Monoclonal Antibodies
 
 
ALX-804-155 Revised 27-Feb-07
Monoclonal Antibody to RIP3 (human) (Rippy-3)
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SYNONYMS anti-Receptor-interacting Protein 3 (human) MAb (Rippy-3)
anti-Receptor-interacting Serine/Threonine Protein Kinase 3 (human) MAb (Rippy-3)
anti-RIP-like Protein Kinase 3 (human) MAb (Rippy-3)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY RIP Kinases
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ALX-804-155-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Rippy-3
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIP3 (receptor-interacting protein 3).
SPECIFICITY: Recognizes human RIP3.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-608.
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General Information
Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNF-R1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3 [1-3]. RIP3 contains an N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNF-R1, mediates TNF-R1 induced apoptosis, and attenuates RIP and TNF-R1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues [2,3]. Meanwhile RIP4 has been identified and initially characterized. These recent data suggest that RIP4 is involved in NF-κB and JNK signalling and that caspase-dependent processing of RIP4 may negatively regulate NF-κB-dependent pro-survival or pro-inflammatory signals [4].
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB: P.W. Yu, et al.; Curr. Biol. 9, 539 (1999) Abstract
[2] RIP3, a novel apoptosis-inducing kinase: X. Sun, et al.; J. Biol. Chem. 274, 16871 (1999) Abstract; Full Text
[3] Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-kappaB: N.J. Pazdernik, et al.; Mol. Cell. Biol. 19, 6500 (1999) Abstract; Full Text
[4] RIP4 (DIK/PKK), a novel member of the RIP kinase family, activates NF-kappa B and is processed during apoptosis: E. Meylan, et al.; EMBO Rep. 3, 1201 (2002) Abstract; Full Text
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Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-831 Revised 22-Sep-05
Monoclonal Antibody to SARM (human) (Sarmy-1)
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SYNONYMS anti-TIR-1 (human) MAb (Sarmy-1)
anti-Sterile α and TIR Motif-containing Protein 1 (human) MAb (Sarmy-1)
anti-SAM and ARM-containing Protein (human) MAb (Sarmy-1)
anti-Sterile α Motif and Armadillo Repeat-containing Protein (human) MAb (Sarmy-1)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Toll-IL-1 Receptor Adaptors / Related Products
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ALX-804-831-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Sarmy-1
ISOTYPE: Mouse IgG2b
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
IMMUNOGEN: Recombinant human SARM (aa 93-292).
SPECIFICITY: Recognizes human SARM.
APPLICATION: Immunoprecipitation (1:200)
Note: Does not work for Western Blot.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
General Information
In mammals, TIR domain adapters, including Myd88, TIRAP, TICAM-1 (also called TRIF), and TRAM, act in Toll-like receptor (TLR) signaling pathway. The last member of this family called SARM (sterile and armadillo motifs) is highly conserved but has no known function in any organism. SARM is the only mammalian TIR-domain adapter proteins conserved in C. elegans, suggesting that SARM is the most ancient member of this class of signaling proteins. Overexpression of SARM fails to activate either NF-kB- or IRF-3-dependent reporter gene expression, two transcription factors that are sensitive to TLR signaling, suggesting that SARM is functionally distinct from other mammalian TIR domain-containing proteins.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Requirement for a conserved Toll/interleukin-1 resistance domain protein in the Caenorhabditis elegans immune response: N.T. Liberati, et al.; PNAS 101, 6593 (2004) Abstract
TLR-independent control of innate immunity in Caenorhabditis elegans by the TIR domain adaptor protein TIR-1, an ortholog of human SARM: C. Couillault, et al.; Nat. Immunol. 5, 488 (2004) Abstract
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Monoclonal Antibodies
 
 
ALX-804-372 Revised 22-May-08
Monoclonal Antibody to TANK-binding Kinase 1 (108A429)
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SYNONYMS anti-TBK1 MAb (108A429)
anti-NAK MAb (108A429)
anti-NF-κB-activating Kinase MAb (108A429)
anti-T2K MAb (108A429)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY IkB Kinases [IKKs] / Related Products
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ALX-804-372-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 108A429
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 563-577 (YYQFKKDKAERRLAYC) of human TBK1 (TANK-binding kinase 1).
SPECIFICITY: Recognizes human, mouse, rat, dog and bovine TBK1. Detects a band of ~80kDa by Western blot.
APPLICATION: Western Blot (1-2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
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Product Description
TANK-binding Kinase 1 (TBK1) is a member of the IKK family. TANK (TRAF-associated NF-κB activator) interacts with TBK1 and activates NF-κB in a kinase-dependent manner [1].
Product Specific Literature References
[1] NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase: J.L. Pomerantz & D. Baltimore; EMBO J. 18, 6694 (1999) Abstract; Full Text
[2] IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway: K.A. Fitzgerald, et al.; Nat. Immunol. 4, 491 (2003) Abstract
[3] Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I: R. Sumpter, Jr., et al.; J. Virol. 79, 2689 (2005) Abstract; Full Text
[4] Gene expression and antiviral activity of alpha/beta interferons and interleukin-29 in virus-infected human myeloid dendritic cells: P. Osterlund, et al.; J. Virol. 79, 9608 (2005) Abstract
Further Categories Containing This Product:
Antiviral Signalling / Related Products • Monoclonal Antibodies
 
 
ALX-804-829 Revised 31-Oct-06
Monoclonal Antibody to TIRAP (human) (Sebi-1)
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SYNONYMS anti-Toll-interleukin 1 Receptor Domain-containing Adapter Protein (human) MAb (Sebi-1)
anti-Mal (human) MAb (Sebi-1)
anti-MyD88 Adapter-like Protein (human) MAb (Sebi-1)
PRODUCT LINE Inflammation
PRODUCT CATEGORY Toll-IL-1 Receptor Adaptors / Related Products
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ALX-804-829-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Sebi-1
ISOTYPE: Rat IgG2a
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. Purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human TIRAP (Mal) (aa 1-215).
SPECIFICITY: Recognizes human TIRAP (Mal).
APPLICATION: Immunoprecipitation (1:200)
Western Blot (1:500)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
General Information
Toll-like receptors (TLRs) play an essential role in the detection and elimination of invading pathogens. Recognition of microbial components by TLRs triggers activation of the innate immune system. Upon stimulation, these receptors interact with specific TIR domain-containing adaptor proteins. Five such adaptors have been discovered to date, MyD88, Trif (TIR-domain-containing adaptor inducing interferon-β), TIRAP (TIR domain-containing adaptor protein)/Mal (MyD88 adaptor-like), TRAM (Trif-related adaptor molecule) and SARM (SAM and ARM-containing protein). MyD88 is common to all TLR-mediated pathways, which lead to the production of inflammatory cytokines, whereas TRIF mediates induction of IFN-β in TLR3 and TLR4 signalling pathways. TIRAP/Mal is implicated in the TLR2- and TLR4-mediated MyD88-dependent signalling pathway. TRAM is specifically involved in the TLR4-mediated TRIF-dependent pathway. Thus, TIR domain-containing adaptors provide specificity of TLR signalling.
General Literature References
TIRAP: an adapter molecule in the Toll signaling pathway: T. Horng, et al.; Nat. Immunol. 2, 835 (2001) Abstract; Full Text
Inferences, questions and possibilities in Toll-like receptor signalling: B. Beutler; Nature 430, 257 (2004) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-132 Revised 21-Aug-08
Monoclonal Antibody to Tollip (Kimmy-1)
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PRODUCT LINE Inflammation
PRODUCT CATEGORY Toll-IL-1 Receptor Adaptors / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-132-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Kimmy-1
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE).
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse Tollip.
SPECIFICITY: Recognizes human and mouse Tollip.
APPLICATION: Immunoprecipitation
Western Blot (5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at +4°C undiluted.
HANDLING: Do not freeze/thaw.
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Product Specific Literature References
Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor: K. Burns, et al.; Nat. Cell Biol. 2, 346 (2000) Abstract
Threonine 66 in the death domain of IRAK-1 is critical for interaction with signaling molecules but is not a target site for autophosphorylation: D. Neumann, et al.; J. Leukoc. Biol. 84, 807 (2008) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-154 Revised 16-Jun-08
Monoclonal Antibody to Tollip (Kimmy-2)
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PRODUCT LINE Inflammation
PRODUCT CATEGORY Toll-IL-1 Receptor Adaptors / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-154-C100   100 µg 360.00 USD Add To Cart
ALX-804-154B-C100 Biotin 100 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: Kimmy-2
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue supernatant.
CONCENTRATION: 1mg/ml
PURITY: 95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse Tollip (aa 1-274).
SPECIFICITY: Recognizes mouse and human (weak) Tollip.
APPLICATION: Immunocytochemistry (1:100)
Immunoprecipitation
Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.
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