ALEXIS Biochemicals: KillerTRAIL™, Soluble (human) (recombinant) (BULK)

ALX-201-123

Revised 06-Jun-08

KillerTRAIL™, Soluble (human) (recombinant) (BULK)
SYNONYMS	Apo-2L, Soluble (human) (recombinant) TNFSF 10, Soluble (human) (recombinant) CD253, Soluble (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	TRAIL [Apo-2L]

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-123-C500		500 µg	1'400.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide.
CONCENTRATION:	0.5mg/ml.
PURITY:	≥99% (SDS-PAGE)
PURITY DETAIL:	Affinity purified.
FORMULATION:	Liquid. In 20mM HEPES, pH 7.9, containing 120mM sodium chloride, 0.01% Tween 20, 1% sucrose, 1mM DTT.
ENDOTOXIN CONTENT:	<0.2EU/µg protein (LAL test; Bio Whittaker).
RECONSTITUTION:	For a special storage and dilution buffer see KillerTRAIL™ Storage and Dilution Buffer (Prod. No. ALX-505-005).
SPECIFICITY:	Binds to human and mouse TRAIL receptors and osteoprotegerin (OPG).
BIOLOGICAL ACTIVITY:	Induces apoptosis in a concentration range of 10-100ng/ml. Note: This protein does not require a cross-linking enhancer for its potent biological activity. For cell lines that require extensive cross-linking of the TRAIL-Rs for killing (e.g. Jurkat) use SuperKillerTRAIL™ (Prod. No. ALX-201-115).
SPECIFIC ACTIVITY:	ED₅₀: 20ng/ml (BJAB cells)
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.

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Product Specific Literature References

The cytokines tumor necrosis factor-alpha (TNF-alpha ) and TNF-related apoptosis-inducing ligand differentially modulate proliferation and apoptotic pathways in human keratinocytes expressing the human papillomavirus-16 E7 oncoprotein J.R. Basile, et al.; J. Biol. Chem. 276, 22522 (2001) Abstract; Full Text
The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines: Q. Liao, et al.; FEBS Lett. 503, 151 (2001) Abstract; Full Text
TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors: M. Plasilova, et al.; Leukemia 16, 67 (2002) Abstract
TRAIL and its receptors in the colonic epithelium: a putative role in the defense of viral infections: J. Sträter, et al.; Gastroenterology 122, 659 (2002) Abstract
Chronic lymphocytic leukemic cells exhibit apoptotic signaling via TRAIL-R1: M. MacFarlane, et al.; Cell Death Differ. 12, 773 (2005) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P50591: TRAIL (human)
AfCS Signalling Gateway link A002862: TRAIL (mouse)

ALX-505-005	KillerTRAIL™ Storage and Dilution Buffer
ALX-804-296	Monoclonal Antibody to TRAIL (human) (2E5)
ALX-201-115	SuperKillerTRAIL™, Soluble (human) (recombinant)