ALEXIS Biochemicals: LIF (mouse) (recombinant)

ALX-201-242

Revised 07-Aug-06

LIF (mouse) (recombinant)
SYNONYMS	Leukemia Inhibitory Factor (mouse) (recombinant)
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	Cell Differentiation

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-242-L001		1 ml	280.00 USD

Product Specification

MW:	~20kDa
SOURCE/HOST:	Produced in E. coli.
QUANTITY:	10⁷U
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In PBS containing 0.5% BSA. Contains no preservatives.
BIOLOGICAL ACTIVITY:	Induces macrophage differentiation and proliferation of hematopoietic stem cells; maintains indifferentiated state of of embryonic stem (ES) cell cultures; inhibits proliferation of the mouse myeloid cell line M1.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 6 months when stored at +4°C.
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells: R.L. Williams, et al.; Nature 336, 684 (1988) Abstract
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells: C.C. Shih, et al.; Blood 95, 1957 (2000) Abstract
Leukemia inhibitory factor reduces body fat mass in ovariectomized mice: J.O. Jansson, et al.; Eur. J. Endocrinol. 154, 349 (2006) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Maintenance of ES Cells
1’000U/ml are recommended to maintain indifferentiated ES cells, R1 or E14 cells.
M1Growth Inhibition Assay:
50units/ml are defined as the concentration of LIF that induces 50% of differentiated cells in tissue culture medium.

Further Categories Containing This Product:

Recombinant Proteins/Fusion Proteins

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