ALEXIS Biochemicals: LIGHT, Soluble (human) Detection Set [For ELISA Application]

APO-54N-029

Revised 02-Apr-08

LIGHT, Soluble (human) Detection Set [For ELISA Application]
SYNONYMS	HVEML, Soluble (human) Detection Set [For ELISA Application] TNFSF 14, Soluble (human) Detection Set [For ELISA Application] CD258, Soluble (human) Detection Set [For ELISA Application]
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	HVEM & LIGHT / LT-betaR / DcR3 / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-029-KI01		1 Set	540.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SENSITIVITY:	0.2ng/ml (range 0 to 20ng/ml).
QUANTITY:	5x96 wells
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).
APPLICATION:	For the quantitative determination of human LIGHT (HVEML; TNFSF 14) from biological fluids (serum, plasma and cell culture supernatant).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C

General Information

LIGHT (derived from "homologous to Lymphotoxins, exhibits Inducible expression, and competes with Herpes simplex virus (HSV) Glycoprotein D for Herpes virus entry mediator (HVEM /TR2), a receptor expressed by T lymphocytes")is a 29 kDa type 2 transmembrane protein that belongs to the TNF superfamily [1, 2]. It is expressed in granulocytes, monocytes, platelets, immature dendritic cells and activated T cells. LIGHT is active either as membrane-anchored or secreted (soluble) forms. Three receptors of the TNF superfamily: lymphotoxin receptor (LTR), herpesvirus entry mediator (HVEM), and decoy receptor 3 (DcR3) binds to the ligand. LIGHT is involved in T-cell regulation and dendritic cell maturation [3]. It triggers several signaling pathways such as NF-κB, non-canonical NF-κB, Stat3, ERK5 / BMK1 and JNK [4]. LIGHT is released from platelets and induces a proinflammatory state in vascular endothelial cells [5]. It suppress tumor formation in vivo by inducing apoptotic cell death. Embryonic stem (ES) cells are differentiated in the presence of LIGHT [6]. Mice overexpressing LIGHT spontaneously develop severe autoimmune disease. LIGHT has been reported to increase in Rhumatoid Arthritis (RA) patients and to induce a RANKL dependent and -independent osteoclasts formation [7].
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link O43557: Tumor Necrosis Factor Ligand Superfamily Member 14 (human)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

[1] LIGHT-HVEM signaling and the regulation of T cell-mediated immunity: S.W. Granger & S. Rickert; Cytokine Growth Factor Rev. 14, 289 (2003) Abstract
[2] LIGHT, a new member of the TNF superfamily, and lymphotoxin alpha are ligands for herpesvirus entry mediator: D.N. Mauri, et al. ; Immunity 8, 21 (1998) Abstract
[3] LIGHT, a TNF-like molecule, costimulates T cell proliferation and is required for dendritic cell-mediated allogeneic T cell response: K. Tamada, et al. ; J. Immunol. 164, 4105 (2000) Abstract
[4] LIGHT, a member of the TNF superfamily, activates Stat3 mediated by NIK pathway: N. Nadiminty, et al. ; BBRC 359, 379 (2007) Abstract
[5] Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes: K. Otterdal, et al. ; Blood 108, 928 (2006) Abstract
[6] LIGHT induces differentiation of mouse embryonic stem cells associated with activation of ERK5: G.M. Zou, et al. ; Oncogene 25, 463 (2006) Abstract
[7] LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis: J.R. Edwards, et al. ; Arthritis Rheum. 54, 1451 (2006) Abstract

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