ALEXIS Biochemicals: LPS from Salmonella minnesota R595 (Re) (TLRgrade™) (Ready-to-Use)

ALX-581-008

Revised 10-Sep-08

LPS from Salmonella minnesota R595 (Re) (TLRgrade™) (Ready-to-Use)
SYNONYMS	Lipopolysaccharide from Salmonella minnesota R595 (Re) (TLRgrade™) (Ready-to-Use)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	Lipopolysaccharides [LPS] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-581-008-L002		2 ml	155.00 USD

Product Specification

RTECS:	UX2990000
SOURCE/HOST:	Rough (R)-form LPS, isolated and purified from Salmonella minnesota R595 (Re mutant) by a modification of the PCP extraction method [1,2], converted to the uniform sodium salt form [3] and dissolved in sterile pyrogen-free double distilled water.
CONCENTRATION:	1mg/ml
PURITY:	Absence of detectable protein or DNA contaminants with agonistic TLR activity.
FORMULATION:	Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
ACTIVITY:	Strong activator of Toll-like receptor (TLR) 4. Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice. No further re-extraction required. Smooth (S)-form LPS are commonly the preferred choice for whole animal studies, whereas Rough (R)-form LPS are primarily used in cellular in vitro activation studies.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored at +4°C.
HANDLING:	Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Avoid inhalation of LPS and prevent these compounds from entering the bloodstream. Keep sterile. Use must be restricted to qualified personnel.
HAZARD:	HARMFUL. HIGHLY PYROGENIC.

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Product Specific Literature References

[1] A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969) Abstract
[2] Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Luderitz; Methods in Carbohydrate Chem. 9, 11 (1993)
[3] Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Luderitz; Eur. J. Biochem. 54, 603 (1975) Abstract
[4] Oligosaccharides of Hyaluronan activate dendritic cells via toll-like receptor 4: C. Termeer, et al.; J. Exp. Med. 195, 99 (2002) Abstract; Full Text
[5] CD36 is a sensor of diacylglycerides: K. Hoebe, et al.; Nature 433, 523 (2005) Abstract
[6] CD14 is required for MyD88-independent LPS signaling: Z. Jiang, et al.; Nat. Immunol. 6, 565 (2005 6, 565 (2005) Abstract
[7] Toll-like receptor 9-induced type I IFN protects mice from experimental colitis: K. Katakura, et al.; J. Clin. Invest. 115, 695 (2005) Abstract; Full Text
[8] R-form LPS, the master key to the activation ofTLR4/MD-2-positive cells: M. Huber, et al.; Eur. J. Immunol. 36, 701 (2006) Abstract

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