ALEXIS Biochemicals: LTβR (human):Fc (human) (recombinant)

ALX-522-041

Revised 20-Jul-06

LTβR (human):Fc (human) (recombinant)
SYNONYMS	Tumor Necrosis Factor Receptor 2-related Protein TNFRSF 3 (human):Fc (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	HVEM & LIGHT / LT-betaR / DcR3 / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-041-C050		50 µg	360.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
MW:	Migrates as a doublet at ~56kDa and ~60kDa (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The cysteine-rich region of human LTβR (aa 1-220) is fused to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute in 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds human and mouse LIGHT and LTα1β2.
BIOLOGICAL ACTIVITY:	Inhibits human LIGHT- and LTα1β2-mediated effects such as cytotoxic effects on HT29 target cells.
APPLICATION:	Detection of human membrane-bound LIGHT in combination with PAb to human IgG1.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Specific Literature References

The lymphotoxin-β receptor is necessary and sufficient for LIGHT-mediated apoptosis of tumor cells: I.A. Rooney, et al.; J. Biol. Chem. 275, 14307 (2000) Abstract; Full Text
Lymphotoxin-mediated regulation of gammadelta cell differentiation by alphabeta T cell progenitors: B. Silva-Santos, et al.; Science 307, 925 (2005) Abstract
Interactions of tumor necrosis factor (TNF) and TNF receptor family members in the mouse and human: C. Bossen, et al.; J. Biol. Chem. 281, 13964 (2006) Abstract; Full Text

General Information

LTβR is a 75kDa type I transmembrane protein belonging to the TNF receptor family. It is 414 aa long and contains 4 cysteine-rich repeats in its extracellular domain. It is expressed on monocytes, fibroblasts, stromal and muscle cells. It is known to activate nuclear factor NF-κB, to interact with NIK and to induce cell death via TNF receptor-associated factor 3 (TRAF3). LTβR has two known ligands: LIGHT and LTα1β2. It has been shown to be involved in splenic architecture development, lymph node genesis and affinity maturation, as well as in the development of diabetes and colitis. It has been implicated in the induction of HIV replication and impairs prion replication in mice lacking follicular dendritic cells.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P36941: LTβR (human) (precursor) AfCS Signalling Gateway link A001440: LTβR (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.