ALEXIS Biochemicals: Leptin (human) (recombinant)

ALX-201-034

Revised 02-May-06

Leptin (human) (recombinant)
SYNONYMS	OB Gene Product (human) (recombinant)
PRODUCT LINE	Obesity & Adipokines
PRODUCT CATEGORY	Leptin/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-034-M001		1 mg	190.00 USD

Product Specification

MW:	~16kDa.
MERCK INDEX:	14: 5443
SOURCE/HOST:	Produced in E. coli. Homologous to human serum leptin.
PURITY:	≥98% (SDS-PAGE)
FORMULATION:	Lyophilized from sterile filtered solution in 10mM sodium citrate, pH 4.0.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein.
RECONSTITUTION:	Reconstitute with sterile water or 0.4% sodium bicarbonate, pH 8-9. Do not reconstitute to less than 0.1mg/ml. Further dilutions should be made with medium containing 0.1% HSA or BSA.
BIOLOGICAL ACTIVITY:	Induces proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted protein is stable for 4 weeks when stored at +4°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.

General Information

Leptin, the product of the ob (obese) gene, is a 16kDa protein consisting of 146 amino acid residues. Leptin is produced in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and regulation of body weight. It targets the central nervous system, particularly hypothalamus, affecting food intake. Leptin levels are high in most obese individuals. Studies have shown that it may also influence reproductive function.

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P41159: Leptin (human) (precursor)

General Literature References

Leptin and the regulation of body weight in mammals: J.M. Friedman and J.L. Halaas; Nature 395, 763 (1998), review Abstract
Mutation screening and identification of a sequence variation in the human ob gene coding region: R.V. Considine, et al.; BBRC 220, 735 (1996) Abstract
Serum immunoreactive leptin concentrations in normal-weight and obese humans  : R.V. Considine, et al. ; New Engl. J. Med. 334, 292 (1996) Abstract
Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects  : M. Maffei, et al.; Nature Medicine 1, 1155 (1995) Abstract
Effects of the obese gene product on body weight regulation in ob/ob mice  : M.A. Pelleymounter, et al. ; Science 269, 540 (1995) Abstract
Evidence against either a premature stop codon or the absence of obese gene mRNA in human obesity  : R.V. Considine, et al. ; J. Clin. Invest 95, 2986 (1995) Abstract
Overexpression of the obese (ob) gene in adipose tissue of the human subjects  : F. Lönnqvist, et al. ; Nature Medicine 1, 950 (1995) Abstract
Positional cloning of the mouse obese gene and its human homologue  : Y. Zhang, et al.; Nature 372, 425 (1994) Abstract

Further Categories Containing This Product:

Recombinant Proteins/Fusion Proteins

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