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ALX-804-590 Revised 14-Jul-08
M30 CytoDEATH™ Monoclonal Antibody
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Cytokeratin-based Apoptosis Detection
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-590-T200   200 tests 465.00 USD Add To Cart
ALX-804-590B-T200 Biotin 200 tests 495.00 USD Add To Cart
ALX-804-590F-T200 FITC 200 tests 495.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Monkey
Others
CLONE: M30
ISOTYPE: Mouse IgG2b
CONCENTRATION: M30 & M30-FITC: 0.1mg/ml
M30-Biotin: 0.5mg/ml
FORMULATION: Liquid. In 200μl PBS containing 0.1% BSA, PEG, sucrose and 0.09% sodium azide.
IMMUNOGEN: CK18 (cytokeratin 18) fragments purified from supernatant from human carcinoma cell line WiDr CCL218.
SPECIFICITY: Recognizes the CK18-Asp396 neo-epitope (M30) on human, monkey and bovine caspase-cleaved CK18. Does not cross-react with intact CK18.
APPLICATION: M30 & M30-Biotin:
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (frozen sections; paraffin sections)
Western Blot

M30-FITC:
Flow Cytometry
Immunocytochemistry

A dilution of 1:100 in incubation buffer is generally recommended. Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Immunocytochemical detection and mapping of a cytokeratin 18 neo-epitope exposed during early apoptosis: M.P. Leers, et al.; J. Pathol. 187, 567 (1999) Abstract
Apoptosis of circulating tumor cells in prostate cancer patients: C. J. Larson, et al.; Cytometry A. 62, 46 (2004) Abstract
Uncovering the roles of intermediate filaments in apoptosis: N. Marceau; Methods Cell Biol. 78, 95 (2004) Abstract
Maternal smoking, intrauterine growth restriction, and placental apoptosis: C. Vogt Isaksen; Pediatr. Dev. Pathol. 7, 433 (2004) Abstract
Modular effects of estradiol on ethanol-induced apoptosis in human intestinal epithelial cells: K. Asai, et al.; Scand. J. Gastroenterol. 40, 326 (2005) Abstract
Apoptosis resistance in Barrett’s esophagus: ex vivo bioassay of live stressed tissues: K. Dvorakova, et al.; Am. J. Gastroenterol. 100, 424 (2005) Abstract
Oncosis represents the main type of cell death in mouse models of cholestasis: P. Fickert, et al.; J. Hepatol. 42, 378 (2005) Abstract
Mcl-1 expression in gestational trophoblastic disease correlates with clinical outcome: a differential expression study: P.Y. Fong, et al.; Cancer 103, 268 (2005) Abstract
Assessment of apoptosis by immunohistochemical markers compared to cellular morphology in ex vivo-stressed colonic mucosa: H. Holubec, et al.; J. Histochem. Cytochem. 53, 229 (2005) Abstract
Inhibition of bcl-2 enhances the efficacy of chemotherapy in renal cell carcinoma: I. Kausch, et al.; Eur. Urol. 47, 703 (2005) Abstract
Expression of tumour necrosis factor-related apoptosis-inducing ligand death receptors in sporadic and hereditary colorectal tumours: Potential targets for apoptosis induction: J.J. Koornstra, et al.; Eur. J. Cancer 41, 1195 (2005) Abstract
Expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in human preeclamptic placenta: possible implications in the process of trophoblast apoptosis: H. Lee, et al.; Placenta 26, 226 (2005) Abstract
Role of bax mutations in apoptosis in colorectal cancers with microsatellite instability: C. Miquel, et al.; Am. J. Clin. Pathol. 123, 562 (2005) Abstract
Fas ligand expression by maternal decidual cells is negatively correlated with the abundance of leukocytes present at the maternal-fetal interface: Q. Qiu, et al.; J. Reprod. Immunol. 65, 121 (2005) Abstract
Differential susceptibility of metastatic and primary oral cancer cells to TRAIL-induced apoptosis: N. Vigneswaran, et al.; Int. J. Oncol. 26, 103 (2005) Abstract
Effects of vitamin restriction and supplementation on rat intestinal epithelial cell apoptosis: B. Vijayalakshmi, et al.; Free Radic. Biol. Med. 38, 1614 (2005) Abstract
Expression levels of Akt in nimesulide-treated squamous carcinoma cell lines of the head and neck: L. Vormittag, et al.; Oncol. Rep. 13, 207 (2005) Abstract
For a comprehensive bibliography please click here:
General Information
BACKGROUND/TECHNICAL INFORMATION Cytokeratin 18 expression has been shown in but is not limited to cells and tissues from lung, breast, intestine, gut, colon, kidney, pancreas, bladder, trophoblasts ... etc.
The M30 CytoDEATH™ monoclonal antibody has been shown in numerous independent studies to be specific to the CK18-Asp396 caspase cleavage site (neo-epitope (M30)) and represents a well-established, validated apoptosis marker that does not react with viable or necrotic cells.
M30 CytoDEATH™ detects human, monkey and bovine apoptotic epithelial cells and tissues and has been validated for many applications including immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) tissues.
Apoptosis detection using M30 CytoDEATH™ has been described in several publications to serve as a superior technique compared to TUNEL or detection of γH2AX phosphorylation. Assessment of apoptosis by immunohistochemical markers compared to cellular morphology in ex vivo‑stressed colonic mucosa under conditions of DNA-damage may be repaired and results in false-positive counts of apoptotic cells. In addition has modest or transient caspase activation without the cleavage of death substrates been attributed to non-suicidal processes such as proliferation, differentiation and inflammation.

For more information on procedures and materials required please see also the Original Manufacturers Product Description
(click here to download pdf for M30 CytoDEATH™ Monoclonal Antibody)
(click here to download pdf for M30 CytoDEATH™ Monoclonal Antibody (Biotin))
(click here to download pdf for M30 CytoDEATH™ Monoclonal Antibody (FITC))
MANUFACTURER Manufactured by Peviva AB.
Further Categories Containing This Product:
Tumor MarkersMonoclonal Antibodies
 
 

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