ALEXIS Biochemicals: MMP-2/TIMP-2 Proenzyme Complex (human)

ALX-200-420

Revised 18-Mar-08

MMP-2/TIMP-2 Proenzyme Complex (human)
SYNONYMS	Matrix Metalloproteinase 2/Tissue Inhibitor of Metalloproteinase 2 Complex (human)
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	MMPs

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-200-420-C005		5 µg	166.00 USD

Product Specification

EC:	3.4.24.24 (MMP-2)
SOURCE/HOST:	Isolated from human rheumatoid synovial fibroblasts. Requires activation.
CONCENTRATION:	~100µg/ml
PURITY:	≥95% (SDS-PAGE, Western blot)
PURITY DETAIL:	No other MMP contaminants are detectable.
FORMULATION:	Liquid. In 50mM TRIS-HCl pH 7.0, 200mM sodium chloride, 5mM CaCl₂, 1µM ZnCl₂, 0.05% NaN₃ and 0.05% BRIJ 35.
SPECIFIC ACTIVITY:	≥30mU/mg protein (Y. Masui, et al.; Biochem. Med. 17, 215 (1977)). One unit is defined as the amount of enzyme that hydrolyzes 1µmol Dnp-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-20°C
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Do always keep the enzyme on ice.
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Synthetic substrates for vertebrate collagenase: Y. Masui, et al.; Biochem. Med. 17, 215 (1977) Abstract
The complex between a tissue inhibitor of metalloproteinases (TIMP-2) and 72-kDa progelatinase is a metalloproteinase inhibitor: H. Kolkenbrock, et al.; Eur. J. Biochem. 198, 775 (1991) Abstract
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract
Activation of progelatinase A and progelatinase A/TIMP-2 complex by membrane type 2-matrix metalloproteinase: H. Kolkenbrock, et al.; Biol. Chem. 378, 71 (1997) Abstract

General Information

MMP-2 and TIMP-2 form a stable, but non-covalent 1:1 stoichiometric complex. The complex inhibits active matrix MMP's like collagenases and gelatinases and shows proteolytic activity after activation with APMA (4-aminophenylmercury acetate).

BACKGROUND/TECHNICAL INFORMATION

Precursor enzyme needs activation using 2mM AMPA (aminophenylmercuric acetate) or 1mM mersalylic acid for 60-120 min. at 37°C. Do not use trypsin for activation! Do not dilute the enzyme for activation!

The active enzyme is inhibited by TIMP-1 (tissue inhibitor of matrix metalloproteinase-1) and by chelators of divalent cations like EDTA or o-phenantroline.

Swiss-Prot link: MMP-2 (human) (precursor) (P08253), TIMP-2 (human) (precursor) (P16035)

Further Categories Containing This Product:

Natural Proteins • Tissue Inhibitors of Matrix Metalloproteinases [TIMPs]/Related Products • Enzymes

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