ALEXIS Biochemicals: MMP-8 Proenzyme (human)

ALX-200-421

Revised 07-Nov-07

MMP-8 Proenzyme (human)
SYNONYMS	Matrix Metalloproteinase 8 (human) Neutrophil Collagenase (human)
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	MMPs

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-200-421-C005		5 µg	136.00 USD

Product Specification

MW:	~85kDa.
EC:	3.4.24.34
SOURCE/HOST:	Isolated from stimulated human neutrophil granulocytes (buffy coat). Requires activation.
CONCENTRATION:	100µg/ml
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. In 50mM TRIS-HCl, pH 7.0, containing 200mM NaCl, 5mM CaCl₂, 1µM ZnCl₂, 0.05% BRIJ 35, and 0.05% sodium azide.
SPECIFIC ACTIVITY:	≥60mU/mg protein (Y. Masui, et al.; Biochem. Med. 17, 215 (1977)). One unit is defined as the amount of enzyme that hydrolyzes 1µmol 2,4-dinitrophenyl-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Stable for 1 week when stored at +4°C and for serveral weeks when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles.

Product Description

MMP-8 is a glycoprotein containing complex N-linked oligosaccharides. It hydrolyzes type I over type II, and III collagens. Activated MMP-8 is inhibited by TIMP-1 (Prod. No. ALX-200-426).

Product Specific Literature References

Synthetic substrates for vertebrate collagenase: Y. Masui, et al.; Biochem. Med. 17, 215 (1977) Abstract
Secreted forms of human neutrophil collagenase: K.A. Hasty, et al.; J. Biol. Chem. 261, 5645 (1986) Abstract; Full Text
Characterization of 58-kilodalton human neutrophil collagenase: comparison with human fibroblast collagenase: S.K. Mallya, et al.; Biochemistry 29, 10628 (1990) Abstract
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Precursor enzyme needs activation using 2mM AMPA (aminophenylmercuric acetate) or 1mM mersalylic acid for 60 min. at 37°C. Alternatively use 0.1mM PCMB (p-chloromercuribenzoate) or 10µg/ml trypsin for 20 min. at 25°C; PCMB is substantially more effective.

The active enzyme is inhibited by TIMP-1 (tissue inhibitor of matrix metalloproteinase-1) and by chelators of divalent cations like EDTA or o-phenantroline.

Swiss-Prot link P22894: MMP-8 (human) (precursor)
AfCS Signalling Gateway link A001478: MMP-8 (mouse)

Further Categories Containing This Product:

Natural Proteins • Enzymes

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