ALEXIS Biochemicals: MegaAPRIL™, Soluble (human) (recombinant)

ALX-522-035

Revised 14-Aug-08

MegaAPRIL™, Soluble (human) (recombinant)
SYNONYMS	APRIL, Soluble (human) (recombinant):ACRP30headless (human) A-Proliferation-inducing Ligand (human) (recombinant):ACRP30headless (human) TNFSF 13, Soluble (human) (recombinant):ACRP30headless (human) CD256, Soluble (human) (recombinant):ACRP30headless (human)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	APRIL

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-035-3010		3x10 µg	460.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
MW:	~34kDa (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of human APRIL (aa 88-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a FLAG^®-tag.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY:	Binds to human and mouse BCMA and TACI. Binds to proteoglycans.
BIOLOGICAL ACTIVITY:	Stimulates proliferation of serum-starved NIH 3T3 cells. Stimulates B cell proliferation.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

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Product Specific Literature References

DCs induce CD40-independent immunoglobulin class switching through BLyS and APRIL: M.B. Litinskiy, et al.; Nat. Immunol. 3, 822 (2002) Abstract
APRIL modulates B and T cell immunity: J.V. Stein, et al.; J. Clin. Invest. 109, 1587 (2002) Abstract; Full Text
Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL: B. He, et al.; J. Immunol. 172, 3268 (2004) Abstract; Full Text
Potential role of APRIL as autocrine growth factor for megakaryocytopoiesis: D. Bonci, et al.; Blood 104, 3169 (2004) Abstract; Full Text
BAFF regulates B cell survival by downregulating the BH3-only family member Bim via the ERK pathway: A. Craxton, et al.; J. Exp. Med. 202, 1363 (2005) Abstract
APRIL and BAFF promote increased viability of replicating human B2 cells via mechanism involving cyclooxygenase 2: P.K. Mongini, et al.; J. Immunol. 176, 6736 (2006) Abstract; Full Text
TACI regulates IgA production by APRIL in collaboration with HSPG: D. Sakurai, et al.; Blood 109 , 2961 (2007) Abstract
BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway: T. Endo, et al.; Blood 109, 703 (2007) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link O75888: APRIL (human)
AfCS Signalling Gateway link A000305: APRIL (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth: M. Hahne, et al.; J. Exp. Med. 188, 1185 (1998) Abstract
Interaction of the TNF homologues BLyS and APRIL with the TNF receptor homologues BCMA and TACI: S.A. Marsters, et al.; Curr. Biol. 10, 785 (2000) Abstract
A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth: P. Rennert, et al.; J. Exp. Med. 192, 1677 (2000) Abstract
Tumor necrosis factor (TNF) receptor superfamily member TACI is a high affinity receptor for TNF family members APRIL and BLyS: Y. Wu, et al.; J. Biol. Chem. 275, 35478 (2000) Abstract
APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity: G. Yu, et al.; Nat. Immunol. 1, 252 (2000) Abstract
B-cell maturation protein, which binds the tumor necrosis factor family members BAFF and APRIL, is dispensable for humoral immune responses: S. Xu & K.P. Lam; Mol. Cell. Biol. 21, 4067 (2001) Abstract

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Recombinant Proteins / Fusion Proteins

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