ALEXIS Biochemicals: MegaCD40L™, Soluble (human) (recombinant)

ALX-522-110

Revised 27-Mar-08

MegaCD40L™, Soluble (human) (recombinant)
SYNONYMS	CD40L, Soluble (human) (recombinant):ACRP30headless (mouse) CD154, Soluble (human) (recombinant):ACRP30headless (mouse) TNFSF 5, Soluble (human) (recombinant):ACRP30headless (mouse) gp39, Soluble (human) (recombinant):ACRP30headless (mouse)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	CD40 & CD40L [CD154]/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-110-C010		10 µg	380.00 USD

Product Specification

MW:	~35-40kDa
SOURCE/HOST:	Produced in CHO cells. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG^®-tag.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
BIOLOGICAL ACTIVITY:	Binds to human CD40. Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Images

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Product Description

MegaCD40L™ is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30/adiponectin. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to [CD40L+enhancer] combinations (Prod. No. ALX-850-064).

Product Specific Literature References

Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex: N. Holler, et al.; Mol. Cell. Biol. 23, 1428 (2003) Abstract; Full Text
Impaired CD40L signaling is a cause of defective IL-12 and TNF-alpha production in Sezary syndrome: circumvention by hexameric soluble CD40L: L.E. French, et al.; Blood 105, 219 (2005) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P29965: CD40L (human) Swiss-Prot link Q60994: ACRP30 (mouse) (precursor) FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER	Manufactured by Apotech Corporation.