ALEXIS Biochemicals: Monoclonal Antibody to µ-Calpain (Domain III) (9A4H8D3)

ALX-804-050

Revised 13-Nov-06

Monoclonal Antibody to µ-Calpain (Domain III) (9A4H8D3)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calpains/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-050-R100		100 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Pig Others
CLONE:	9A4H8D3
ISOTYPE:	Mouse IgG1
FORMULATION:	Liquid. Ascites containing 0.05% sodium azide.
IMMUNOGEN:	Purified bovine µ-calpain.
SPECIFICITY:	Recognizes an epitope in domain III (aa 465-520) of µ-calpain from several mammalian sources (e.g. human, mouse, rat, pig, bovine). Detects a band of ~80kDa by Western blot. Does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.
APPLICATION:	Immunocytochemistry (1:100) Immunohistochemistry (1:100) Western Blot (1:2’000) Note: Not recommended for immunoprecipitation.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Description

This antibody has been successfully used in Western blot procedures to detect µ-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, and rat myoblasts, kidney, liver and spleen. Immunofluorescence staining of µ-calpain in porcine LLC-PK1 cells with 804-050 results in diffuse cytoplasmic staining.

Product Specific Literature References

A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells: R.D. Lane, et al.; Exp. Cell Res. 203, 5 (1992) Abstract
Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains: J. Cong, et al.; J. Biol. Chem. 268, 25740 (1993) Abstract; Full Text
Calpain-dependent proteolytic cleavage of the p35 cyclin-dependent kinase 5 activator to p25: G. Kusakawa, et al.; J. Biol. Chem. 275, 17166 (2000) Abstract; Full Text
Calpain is a major cell death effector in selective striatal degeneration induced in vivo by 3-nitropropionate: implications for Huntington’s disease: N. Bizat, et al.; J. Neurosci. 23, 5020 (2003) Abstract; Full Text

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Monoclonal Antibodies

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