ALEXIS Biochemicals: Monoclonal Antibody to µ-Calpain (Domain II) (2H2A7C2)

ALX-804-051

Revised 09-Feb-05

Monoclonal Antibody to µ-Calpain (Domain II) (2H2A7C2)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calpains / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-051-R100		100 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Rat Pig Others
CLONE:	2H2A7C2
ISOTYPE:	Mouse IgG1
FORMULATION:	Liquid. Ascites containing 0.05% sodium azide.
IMMUNOGEN:	Purified bovine µ-calpain.
SPECIFICITY:	Recognizes an epitope in domain II (aa 245-265) of µ-calpain from several mammalian sources (e.g. human, rat, pig, bovine). Detects a band of ~80kDa by Western blot. Does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.
APPLICATION:	Immunocytochemistry (1:25) Western Blot (1:1'000) Note: Not recommended for immunoprecipitation.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

The calpain (Ca²⁺-dependent proteinase or Ca²⁺-activated neutral protease) system consists of two ubiquitous forms of calpain (m-calpain and µ-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered Ca²⁺ homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.
m- and µ-calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa/80 kDa complexes are thought to be inactive proenzymes which, upon binding of Ca²⁺, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

BACKGROUND/TECHNICAL INFORMATION

Immunocytochemical staining of µ-calpain in pig LLC-PK1 cells with Prod. No. ALX-804-051 results in diffuse cytoplasmic staining.

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Monoclonal Antibodies

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