ALEXIS Biochemicals: Monoclonal Antibody to β-Catenin (8E4)

ALX-804-260/1

Revised 09-Oct-08

Monoclonal Antibody to β-Catenin (8E4)
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	Catenin / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-260/1-C100		100 µg	380.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Dog
CLONE:	8E4
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute in 1ml distilled water (15 min., RT).
IMMUNOGEN:	Synthetic peptide corresponding to human β-catenin aa 27-37.
SPECIFICITY:	Recognizes human, mouse, rat (weak) and dog β-catenin. Recognizes an epitope between aa 27-37 and an additional epitope in the C-Terminal region (see [5]). Note: For specific detection of dephospho-β-catenin use Prod. No. ALX-804-259.
APPLICATION:	Flow Cytometry (see Lit. Ref. [3]) Immunocytochemistry (see Lit. Ref. [4]) Immunohistochemistry (paraffin sections) (see Lit. Ref. [3]) Western Blot: 0.5µg/ml for HRPO/ECL detection. Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride. BSA may be used alternatively but milk is not recommended for blocking and dilution!
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL:	Included (Prod. No. ALX-840-022).

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Product Specific Literature References

[2] Sulindac targets nuclear beta-catenin accumulation and Wnt signalling in adenomas of patients with familial adenomatous polyposis and in human colorectal cancer cell lines: E.M. Boon, et al.; Br. J. Cancer 90, 224 (2004) Abstract
[1] Activation of the canonical beta-catenin pathway by histamine: S.H. Diks, et al.; J. Biol. Chem. 278, 52491 (2003) Abstract; Full Text
[3] Illegitimate WNT signaling promotes proliferation of multiple myeloma cells: P.W. Derksen, et al.; PNAS 101, 6122 (2004) Abstract; Full Text
[4] Modulation of intracellular beta-catenin localization and intestinal tumorigenesis in vivo and in vitro by sphingolipids: E.M. Schmelz, et al.; Cancer Res. 61, 6723 (2001) Abstract
[5] Wnt signaling and phosphorylation status of beta-catenin: importance of the correct antibody tools: M. van Noort, et al.; Blood 110, 2778 (2007) Abstract

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