ALEXIS Biochemicals: Monoclonal Antibody to Akt1 (5G12)

ALX-804-633

Revised 05-Jun-07

Monoclonal Antibody to Akt1 (5G12)
SYNONYMS	anti-PKBα MAb (5G12) anti-Protein Kinase Bα MAb (5G12)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Akt [PKB] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-633-C100		100 µg	453.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Rat Dog
CLONE:	5G12
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.1mg/ml
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Liquid. In PBS containing 50% glycerol, 0.09% sodium azide, PEG and sucrose.
IMMUNOGEN:	Synthetic peptide corresponding to human Akt1 (Protein Kinase Bα; PKBα).
SPECIFICITY:	Recognizes human, rat and dog Akt1 (see Technical Note below).
APPLICATION:	Immunohistochemistry Western Blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL:	Included (Prod. No. ALX-840-038).

General Information

BACKGROUND/TECHNICAL INFORMATION

Technical Note: There are three mammalian isoforms of Akt: Akt1 (PKBα), Akt2 (PKBβ), and Akt3 (PKBγ). Akt1 shares 81% and 83% amino acid identity with Akt2 and Akt3, respectively. Although the three isoforms show broad tissue distribution, Akt1 is the most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. In particular, Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. Note that the phosphorylation site slightly differs between the three isoforms.Thus the phosphorylation site Ser⁴⁷³ of Akt1 corresponds to Ser⁴⁷⁴ in Akt2 and Ser⁴⁷² in Akt3 respectively.

Akt1: V⁴⁶¹DSERRPHFPQFS⁴⁷³YSASSTA⁴⁸⁰       Swiss-Prot-Link: P31749
Akt2: L⁴⁶¹LELDQRTHFPQFS⁴⁷⁴YSASIRE⁴⁸¹        Swiss-Prot-Link: P31751
Akt3:  D⁴⁶¹NERRPHFPQFS⁴⁷²YSASGRE⁴⁷⁹       Swiss-Prot-Link: Q9Y243

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Monoclonal Antibodies

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