ALEXIS Biochemicals: Monoclonal Antibody to CD18 (human) (MEM-48)

ALX-805-062

Revised 15-Jan-08

Monoclonal Antibody to CD18 (human) (MEM-48)
SYNONYMS	anti-β2 Integrin (human) MAb (MEM-48)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	CD11a, CD18 [LFA-1] & CD54 [ICAM-1], CD102 [ICAM-2], CD50 [ICAM-3]/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-805-062-C100		100 µg	195.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	MEM-48
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
PURITY DETAIL:	Protein A-affinity purified.
FORMULATION:	Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN:	Leukocytes from a patient suffering from a LGL-type leukemia.
SPECIFICITY:	Recognizes residues 534-546 in cysteine-rich repeat 3 of the human CD18 (β2 integrin). Cross-reacts with all three leukocyte integrins.
APPLICATION:	Flow Cytometry Immunohistochemistry (frozen sections) Immunoprecipitation Western Blot (Recommended dilution: 2µg/ml in buffer containing laurylmaltoside, 2x non-reducing SDS; SDS-PAGE (6% separating gel, 4% stacking gel), positive control: Kg-1a human leukemia cell lysate) Functional Application (induces high-affinity LFA-1 conformation in leukocytes)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Specific Literature References

Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein: V. Bazil, et al.; Folia Biol. (Praha) 36, 41 (1990) Abstract
The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases: T. Cinek and V. Horejsi; J. Immunol. 149, 2262 (1992) Abstract
Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins: L. Ottonello, et al.; Blood 93, 3505 (1999) Abstract; Full Text
Monoclonal Lym-1 antibody-targeted lysis of B lymphoma cells by neutrophils. Evidence for two mechanisms of FcgammaRII-dependent cytolysis: L. Ottonello, et al.; J. Leukoc. Biol. 68, 662 (2000) Abstract
Human leukocytes contain a large pool of free forms of CD18: K. Drbal; BBRC 275, 295 (2000) Abstract
A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins: K. Drbal, et al.; Immunobiology 203, 687 (2001) Abstract
A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells: K. Drbal, et al.; Blood 98, 1561 (2001) Abstract
Regulation of CD18 expression on neutrophils in response to fluid shear stress: S. Fukuda and G.W. Schmid-Schonbein; PNAS 100, 13152 (2003) Abstract; Full Text

Further Categories Containing This Product:

CD Antibodies • Complement Receptors/Related Products • Monoclonal Antibodies

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