ALEXIS Biochemicals: Monoclonal Antibody to CFTR (CF3)

ALX-804-214

Revised 18-Feb-08

Monoclonal Antibody to CFTR (CF3)
SYNONYMS	anti-Cystic Fibrosis Transmembrane Conductance Regulator MAb (CF3) anti-ABCC7 MAb (CF3)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Cl- Channels

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-214-R100		100 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
CLONE:	CF3
ISOTYPE:	Mouse IgM
FORMULATION:	Liquid. Diluted ascites containing 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 103-117(G¹⁰³RIIASYDPDNKEER¹¹⁷) in the first extracellular loop of human and mouse CFTR (cystic fibrosis transmembrane conductance regulator). This sequence is highly conserved in mouse, sheep, bovine and Xenopus laevis CFTR.
SPECIFICITY:	Recognizes human and mouse CFTR. Detects a band of ~170kDa by Western blot.
APPLICATION:	Immunocytochemistry: 1:500. Staining of CFTR in mouse epithelial cells results in cell surface staining, consistent with localization at the plasma membrane. Antibody also detects one or more immunologically related proteins in murine cell line Heb7a that does not contain CFTR mRNA. Immunoprecipitation Western Blot: 1:500 Functional Application: inhibits epithelial uptake of S. typhi in some mouse cell lines.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Production and characterisation of monoclonal and polyclonal antibodies to different regions of thy cystic fibrosis transmembrane conductance regulator: detection of immunologically related proteins: J. Walker, et al.; J. Cell Sci. 108, 2433 (1995) Abstract
Cystic fibrosis transmembrane conductance regulator is vital to sperm fertilizing capacity and male fertility: W.M. Xu, et al.; PNAS 104, 9816 (2007) Abstract

General Information

Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (δ F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake.

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