ALEXIS Biochemicals: Monoclonal Antibody to Caspase-1 (mouse) (1H11)

ALX-804-530

Revised 14-Aug-07

Monoclonal Antibody to Caspase-1 (mouse) (1H11)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-530-C100		100 µg	320.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Mouse
CLONE:	1H11
ISOTYPE:	Rat IgG1
CONCENTRATION:	1mg/ml.
FORMULATION:	Liquid. Protein G sepharose purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN:	p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T²⁰⁶ALEMVKEVKEFAAC²²⁰) of mouse caspase-1.
SPECIFICITY:	Recognizes an epitope within the p20 fragment of mouse caspase-1. Detects a band of ~45kDa by Western blot. Does not cross-react with mouse caspase-11 or -12.
APPLICATION:	ELISA Flow Cytometry Western Blot Other applications not tested.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

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Product Specific Literature References

Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death: V.S. Marsden, et al.; Blood 107, 1872 (2006) Abstract; Full Text

General Information

Capases are a highly conserved family of cysteine proteases and are synthesized as inactive zymogens, which are cleaved at Asp-x sites during apoptosis, generating large and small subunits that heterodimerize to form the active enzyme. Caspase-1 is a member of the ICE/CED-3 family of caspases and is detected in the pro-form as a protein of 45 kDa.
Caspase-1 generates the bioactive form of the pro-inflammatory cytokine IL-1β from its biologically inactive precursor. Overexpression of caspase-1 in cells induces apoptosis, which can be inhibited by cytokine response modifier A (CrmA). Caspase-1 mRNA is predominantly expressed in the spleen and lung and to a lesser extent in skeletal muscle, kidney, testis and heart and within these tissues macrophages preferentially express caspase-1. Caspase-1 deficient mice have no gross abnormalities, however, macrophages from caspase-1-deficient mice are defective in IL-β processing and release.

General Literature References

Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques 25, 824 (1998), Methodology Abstract
Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9: L.A. O'Reilly, et al.; Cell Death Differ. 9, 832 (2002), Methodology Abstract

Further Categories Containing This Product:

Inflammasome • Monoclonal Antibodies

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