ALEXIS Biochemicals: Monoclonal Antibody to Cytochrome c (native) (blocking) (6H2.B4)

ALX-804-295

Revised 21-Sep-05

Monoclonal Antibody to Cytochrome c (native) (blocking) (6H2.B4)
SYNONYMS	anti-Cyt c (native) MAb (blocking) (6H2.B4)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Cytochrome c

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-295-C100		100 µg	293.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat
CLONE:	6H2.B4
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.5mg/ml
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN:	Tissue-derived mouse cytochrome c.
SPECIFICITY:	Recognizes native human, mouse and rat cytochrome c and immunoprecipitates the native protein of ~15kDa. Does not cross-react with denatured cytochrome c.
APPLICATION:	Immunocytochemistry (see [4,6]) Immunohistochemistry (frozen section (2-4µg/ml)) Immunoprecipitation (2µg/10⁶ cells) Functional Application (blocks pro-apoptotic effect of cytosolic cytochrome c) Note: Not recommended for Western blot. For Western blot use MAb to Cytochrome c (denatured) (7H8.2C12) (Prod. No. ALX-804-245).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months when stored at +4°C.

Product Specific Literature References

[1] Common structural features among monoclonal antibodies binding the same antigenic region of cytochrome c: S.C. Goshorn, et al.; J. Biol. Chem. 266, 2134 (1991) Abstract; Full Text
[2] Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c: X. Liu, et al.; Cell 86, 147 (1996) Abstract
[3] XIAP regulates DNA damage-induced apoptosis downstream of caspase-9 cleavage: R. Datta, et al.; J. Biol. Chem. 275, 31733 (2000) Abstract; Full Text
[4] Activation of calpain I converts excitotoxic neuron death into a caspase-independent cell death: S. Lankiewicz, et al.; J. Biol. Chem. 275, 17064 (2000) Abstract; Full Text
[5] Anandamide induces apoptosis in human cells via vanilloid receptors. Evidence for a protective role of cannabinoid receptors: M. Maccarrone, et al.; J. Biol. Chem. 275, 31938 (2000) Abstract; Full Text
[6] Abrin triggers cell death by inactivating a thiol-specific antioxidant protein: S.F. Shih, et al.; J. Biol. Chem. 276, 21780 (2001) Abstract; Full Text
[7] Quantitation of mitochondrial alterations associated with apoptosis: M. Castedo, et al.; J. Immunol. Meth. 265, 39 (2002) Abstract
[8] Curcumin inhibits UV irradiation-induced oxidative stress and apoptotic biochemical changes in human epidermoid carcinoma A431 cells: W.H. Chan, et al.; J. Cell Biochem. 90, 327 (2003) Abstract
[9] Anti-apoptotic effects of curcumin on photosensitized human epidermal carcinoma A431 cells: W.H. Chan and H.J. Wu; J. Cell Biochem. 92, 200 (2004) Abstract
[10] Human colon cancer cells lacking Bax resist curcumin-induced apoptosis and Bax requirement is dispensable with ectopic expression of Smac or downregulation of Bcl-XL: R. Rashmi, et al.; Carcinogenesis 26, 713 (2005) Abstract

Further Categories Containing This Product:

Mitochondria-based Apoptosis Detection • Monoclonal Antibodies

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