ALEXIS Biochemicals: Monoclonal Antibody to Dnmt3a (mouse) (64B1446)

ALX-804-370

Revised 14-Feb-08

Monoclonal Antibody to Dnmt3a (mouse) (64B1446)
SYNONYMS	anti-DNA Methyltransferase-3a MAb (mouse) (64B1446)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	DNA & RNA Methyl & Alkyl Transferases/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-370-C100		100 µg	293.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Mouse
CLONE:	64B1446
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.5mg/ml
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN:	Recombinant mouse Dnmt3a (DNA methyltransferase-3a).
SPECIFICITY:	Recognizes C-terminal epitope aa 705-908 of mouse Dnmt3a [1]. Weakly cross-reacts with human Dnmt3a.
APPLICATION:	Immunocytochemistry (5µg/ml) Immunohistochemistry (1:400) (see [8]) Immunoprecipitation, Chromatin Immunoprecipitation (see [4]) Western Blot (2µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C

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Product Specific Literature References

[1] Lsh, a member of the SNF2 family, is required for genome-wide methylation: K. Dennis, et al.; Genes Dev. 15, 2940 (2001) Abstract; Full Text
[2] A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation: T. Chen, et al.; J. Biol. Chem. 277, 38746 (2002) Abstract
[3] Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin: Q. Yan, et al.; Mol. Cell. Biol. 23, 8416 (2003) Abstract; Full Text
[4] Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer: Y.W. Leu, et al.; Cancer Res. 64, 8184 (2004) Abstract
[5] De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2’-deoxycytidine: M. Oka, et al.; Oncogene 24, 3091 (2005) Abstract
[6] Myc represses transcription through recruitment of DNA methyltransferase corepressor: C. Brenner, et al.; EMBO 24, 336 (2005) Abstract
[7] Cell and stage of transformation-specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model: J.M. Stempak, et al.; Carcinogenesis 26, 981 (2005) Abstract
[8] Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system: J. Feng, et al.; J. Neurosci. Res. 79, 734 (2005) Abstract

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