ALEXIS Biochemicals: Monoclonal Antibody to E-cadherin (ECCD-2)

ALX-804-202

Revised 13-Mar-08

Monoclonal Antibody to E-cadherin (ECCD-2)
SYNONYMS	anti-Uvomorulin MAb (ECCD-2) anti-CD324 MAb (ECCD-2)
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	Cadherin Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-202-C100		100 µg	390.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Others
CLONE:	ECCD-2
ISOTYPE:	Rat IgG2a
SOURCE/HOST:	Obtained by fusing the P3-X63-Ag8 myeloma cell line with splenocytes of Donryu rat immunized with mouse liver E-cadherin fragment.
CONCENTRATION:	2mg/ml after reconstitution.
PURITY DETAIL:	Purified from serum-free culture supernatant by ammonium sulfate precipitation and anion exchange chromatography.
FORMULATION:	Lyophilized from 20mM TBS, pH 7.5, containing 1% BSA. Contains no preservatives.
RECONSTITUTION:	Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl₂, 1.0% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.
IMMUNOGEN:	Mouse liver E-cadherin fragment.
SPECIFICITY:	Recognizes human, mouse and dog E-cadherin. Does not cross-react with bovine or chicken E-cadherin.
APPLICATION:	Immunohistochemistry (frozen sections (10µg/ml), not recommended for paraffin sections) Western Blot (10µg/ml) Functional Application Does not inhibit E-cadherin dependent cell-cell contact and can be used to analyze the mechanism.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Specific Literature References

N-linked oligosaccharides are not involved in the function of a cell-cell binding glycoprotein E-cadherin: Y. Shirayoshi, et al.; Cell Struct. Funct. 11, 245 (1986) Abstract
Transformation of cell adhesion properties by exogenously introduced E- cadherin cDNA: A. Nagafuchi, et al.; Nature 329, 341 (1987) Abstract
The cadherins: cell-cell adhesion molecules controlling animal morphogenesis: M. Takeichi; Development 102, 639 (1988) Abstract
Cadherin cell adhesion receptors as a morphogenetic regulator: M. Takeichi; Science 251, 1451 (1991) Abstract
Recognition of E-cadherin on epithelial cells by the mucosal T cell integrin alpha M290 beta 7 (alpha E beta 7): P.I. Karecla, et al.; Eur. J. Immunol. 25, 852 (1995) Abstract
Role of IQGAP1, a target of the small GTPase Cdc42 and Rac1, in regulation of E-cadherin-mediated cell-cell adhesion: S. Kuroda, et al.; Science 281, 832 (1998) Abstract
The alpha isoform of protein kinase C is involved in signaling the response of desmosomes to wounding in cultured epithelial cells: S. Wallis, et al.; Mol. Biol. Cell. 11, 1077 (2000) Abstract
Recruitment and activation of Rac1 by the formation of E-cadherin- mediated cell-cell adhesion sites: M. Nakagawa, et al.; J. Cell. Sci. 114, 1829 (2001) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Buffers should contain Ca²⁺ to stabilize cadherin antigen. In case of immunohistochemical staining of acetone fixed frozen sections, Ca²⁺ is not necessary.

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