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ALX-804-201 Revised 23-Jun-08
Monoclonal Antibody to E-cadherin (HECD-1)
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SYNONYMS anti-Uvomorulin MAb (HECD-1)
anti-CD324 MAb (HECD-1)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Cadherin Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-201-C100   100 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
CLONE: HECD-1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of BALB/c mouse immunized with human breast tumor cell line MCF-7.
CONCENTRATION: 2mg/ml after reconstitution.
PURITY DETAIL: Ammonium sulfate precipitation and ion exchange chromatography.
FORMULATION: Lyophilized from 10mM PBS, pH 7.4 , containing 1% BSA. Contains no preservatives.
RECONSTITUTION: Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl2, 1.0% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.
IMMUNOGEN: Human breast tumor cell line MCF-7.
SPECIFICITY: Recognizes human and guinea pig E-cadherin. Does not cross-react with rat, dog, bovine or chicken E-cadherin.
APPLICATION: ELISA (2-10µg/ml)
Flow Cytometry (1µg/ml)
Immunohistochemistry (frozen sections (2-10µg/ml), paraffin sections (2-10µg/ml))
Western Blot (2-10µg/ml)
Functional Application (100µg/ml for inhibition of E-cadherin dependent cell-cell contact)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Specific Literature References
Cadherin cell-adhesion molecules in human epithelial tissues and carcinomas: Y. Shimoyama, et al.; Cancer Res. 49, 2128 (1989) Abstract
Expression of immunoreactive E-cadherin adhesion molecules in human cancers: H. Shiozaki, et al.; Am. J. Pathol. 139, 17 (1991) Abstract
Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections: S.R. Shi, et al.; J. Histochem. Cytochem. 39, 741 (1991) Abstract
Cadherin intercellular adhesion molecule in hepatocellular carcinomas: loss of E-cadherin expression in an undifferentiated carcinoma: Y. Shimoyama & S. Hirohashi; Cancer Lett. 57, 131 (1991) Abstract
Expression of E- and P-cadherin in gastric carcinomas: Y. Shimoyama & S. Hirohashi; Cancer Res. 51, 2185 (1991) Abstract
Cadherin cell adhesion receptors as a morphogenetic regulator: M. Takeichi; Science 251, 1451 (1991) Abstract
Immunohistochemical localization of cell adhesion molecule epithelial cadherin in human arachnoid villi and meningiomas: Y. Tohma, et al.; Cancer Res. 52, 1981 (1992) Abstract
J.A. Jankowski, et al.; Int. J. Oncol. 4, 441 (1994)
CD123bright plasmacytoid predendritic cells: progenitors undergoing cell fate conversion?: M.R. Comeau, et al.; J. Immunol. 169, 75 (2002) Abstract; Full Text
Changing etiology of tubal pregnancy following IVF: A. Revel, et al.; Hum. Reprod. 6, 1372 (2008) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Ca2+ should be contained in all buffers to stabilize cadherin antigen.
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
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