ALEXIS Biochemicals: Monoclonal Antibody to ERK2 (6G11)

ALX-804-288

Revised 20-Sep-07

Monoclonal Antibody to ERK2 (6G11)
SYNONYMS	anti-MAPK2 MAb (6G11) anti-MAP Kinase 2 MAb (6G11)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	ERK1 & ERK2/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-288-C100		100 µg	380.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Dog
CLONE:	6G11
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:	Synthetic peptide comprising the pThr-Glu-pTyr motif in activated ERK1 and ERK2.
SPECIFICITY:	Recognizes the C-terminus of human, mouse, rat and dog ERK2. Does not cross-react with ERK1.
APPLICATION:	Immunoprecipitation Western Blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer milk: 2x PBS, 5-8% (w/v) dry milk powder and 0.1% (v/v) Tween 20.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL INCLUDED:	yes

Product Specific Literature References

IL-4-induced priming of human intestinal mast cells for enhanced survival and Th2 cytokine generation is reversible and associated with increased activity of ERK1/2 and c-Fos: A. Lorentz, et al.; J. Immunol. 174, 6751 (2005) Abstract; Full Text

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Monoclonal Antibodies

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