ALEXIS Biochemicals: Monoclonal Antibody to Estrogen Receptor α (33)

ALX-803-004

Revised 15-Apr-08

Monoclonal Antibody to Estrogen Receptor α (33)
SYNONYMS	anti-ERα (33)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Estrogen & Estrogen Receptors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-803-004-C050		50 µg	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Others
CLONE:	33
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.5mg/ml
FORMULATION:	Liquid. Ascites diluted in PBS containing 0.1% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 247-261 (E²⁴⁷VGMMKGGIRKDRRG²⁶¹) of the DNA binding domain of ERα (estrogen receptor α). This sequence is completely conserved in many species including mouse, rat, pig, bovine, sheep, chicken and frog.
SPECIFICITY:	Recognizes both the steroid occupied and unoccupied forms of human, mouse, rat, bovine and monkey ERα. Detects a band of ~67kDa by Western blot. Does not cross-react with androgen receptor, PR (progesterone receptor), GR (glucocorticoid receptor) or ERβ. Does not inhibit binding of ER to nuclei and DNA.
APPLICATION:	Flow Cytometry Gel Supershift Assays. Immunocytochemistry (10µg/ml) Immunohistochemistry (frozen sections; paraffin sections): 5µg/ml. Staining of ERα in mouse Leydig cells results in both nuclear and cytoplasmic staining. Immunoprecipitation Western Blot: 1µg/ml Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:	For Blocking Peptide see Prod. No. ALX-155-028.

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Product Specific Literature References

Development and characterization of monoclonal antibodies to a specific domain of human estrogen receptor: A. Traish, et al.; Steroids 55, 196 (1990) Abstract
Cellular localization of estrogen receptors on neurones in various regions of cultured rat CNS: coexistence with cholinergic and galanin receptors: E. Hosli & L. Hosli; Int. J. Dev. Neurosci. 17, 317 (1999) Abstract
Measurement of estrogen receptors in intact cells by flow cytometry: S. Cao, et al.; Cytometry 41, 109 (2000) Abstract
Histochemical and electrophysiological evidence for estrogen receptors on cultured astrocytes: colocalization with cholinergic receptors: E. Hosli, et al.; Int. J. Dev. Neurosci. 18, 101 (2000) Abstract

General Information

Steroid receptors are a large superfamily of ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Estrogen receptor (ER) has been classified into two distinct isoforms, α and β. ERα is known to interact in a ligand dependent and independent manner with several known coactivators including Receptor-Interacting Protein 140 (RIP140/ERAP140), ERAP160, and Steroid Receptor Coactivator-1 (SRC-1) to enhance transcriptional activity of target genes.

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