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ALX-804-127 Revised 02-Jul-08
Monoclonal Antibody to FLIP (Dave-2)
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SYNONYMS anti-CASPER MAb (Dave-2)
anti-CLARP MAb (Dave-2)
anti-I-FLICE MAb (Dave-2)
anti-Usurpin MAb (Dave-2)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY FLIP
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-127-C100   100 µg 370.00 USD Add To Cart
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Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Dave-2
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
IMMUNOGEN: Recombinant human FLIP (aa 1-480).
SPECIFICITY: Recognizes an epitope (aa 1-200) present in both short (FLIPs) and long (FLIPL) splice variants of human and mouse FLIP.
APPLICATION: Immunoprecipitation
Western Blot
Note: Not recommended for immunohistochemistry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-601.
Product Specific Literature References
Fas engagement induces the maturation of dendritic cells (DCs), the release of interleukin (IL)-1beta, and the production of interferon gamma in the absence of IL-12 during DC-T cell cognate interaction: a new role for Fas ligand in inf: M. Rescigno, et al.; J. Exp. Med. 192, 1661 (2000) Abstract; Full Text
Protein kinase C regulates FADD recruitment and death-inducing signaling complex formation in Fas/CD95-induced apoptosis: M. Gomez-Angelats & J.A. Cidlowski; J. Biol. Chem. 276, 44944 (2001) Abstract; Full Text
Fas aggregation does not correlate with Fas-mediated apoptosis: Y. Lee & E. Shacter; J. Immunol. 167, 82 (2001) Abstract
NF-kB signals induce the expression of c-FLIP: O. Micheau, et al.; Mol. Cell. Biol. 21, 5299 (2001) Abstract; Full Text
Fas-associated death domain protein (FADD) and caspase-8 mediate up-regulation of c-Fos by Fas ligand and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) via a FLICE inhibitory protein (FLIP)-regulated pathway: D. Siegmund, et al.; J. Biol. Chem. 276, 32585 (2001) Abstract; Full Text
Characterization of the human FLICE-inhibitory protein locus and comparison of the anti-apoptotic activity of four different flip isoforms: M. Djerbi, et al.; Scand. J. Immunol. 54, 180 (2002) Abstract
c-FLIP efficiently rescues TRAF-2-/- cells from TNF-induced apoptosis: C. Guiet, et al.; Cell Death Differ. 9, 138 (2002) Abstract
The caspase 8 inhibitor c-FLIP(L) modulates T-cell receptor-induced proliferation but not activation-induced cell death of lymphocytes: S.M. Lens, et al.; Mol. Cell. Biol. 22, 5419 (2002) Abstract
Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Signaling in Activated T Cells Abrogates TRAIL-Induced Apoptosis Upstream of the Mitochondrial Amplification Loop and Caspase-8: T.S. Soderstrom, et al.; J. Immunol. 169, 2851 (2002) Abstract
Expression of the long form of human FLIP by retroviral gene transfer of hemopoietic stem cells exacerbates experimental autoimmune encephalomyelitis

: M. Djerbi, et al.; J. Immunol. 170, 2064 (2003) Abstract; Full Text
Bile acids stimulate cFLIP phosphorylation enhancing TRAIL-mediated apoptosis: H. Higuchi, et al.; J. Biol. Chem. 278, 454 (2003) Abstract; Full Text
Chemotherapy enhances TNF-related apoptosis-inducing ligand DISC assembly in HT29 human colon cancer cells: S. Lacour, et al.; Oncogene 22, 1807 (2003) Abstract
Fas-associated protein with death domain (FADD)-independent recruitment of c-FLIPL to death receptor 5: T.G. Jin, et al.; J. Biol. Chem. 279, 55594 (2004) Abstract; Full Text
Targeting Bcl-x(L) in esophageal squamous cancer to sensitize to chemotherapy plus TRAIL-induced apoptosis while normal epithelial cells are protected by blockade of caspase 9: K. Kim, et al.; Cell Death Differ. 11, 583 (2004) Abstract
The anti-apoptotic factor Bcl-2 can functionally substitute for the B cell survival but not for the marginal zone B cell differentiation activity of BAFF: A. Tardivel, et al.; Eur. J. Immunol. 34, 509 (2004) Abstract
The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover: L. Chang, et al.; Cell 124, 601 (2006) Abstract
A Protective Role for the Human SMG-1 Kinase against Tumor Necrosis Factor-α-induced Apoptosis: V. Oliveira, et al.; J. Biol. Chem. 283, 13174 (2008) Abstract; Full Text

General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Inhibition of death receptor signals by cellular FLIP: M. Irmler, et al.; Nature 388, 190 (1997) Abstract
Apoptosis. Placing death under control: D. Wallach; Nature 388, 123 (1997) Abstract
Inhibition of fas death signals by FLIPs: J. Tschopp, et al.; Curr. Opin. Immunol. 10, 552 (1998) Abstract
Inhibition of death receptor signaling by FLICE-inhibitory protein as a mechanism for immune escape of tumors: L.E. French & J. Tschopp; J. Exp. Med. 190, 891 (1999) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 

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