ALEXIS Biochemicals: Monoclonal Antibody to FOXP3 (mouse) (MF333F)

ALX-804-708

Revised 30-Jan-07

Monoclonal Antibody to FOXP3 (mouse) (MF333F)
SYNONYMS	anti-Forkhead Box Protein P3 (mouse) MAb (MF333F) anti-Scurfin (mouse) MAb (MF333F)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Forkhead Box [FOX] Proteins / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-708-C050		50 µg	220.00 USD
ALX-804-708-C100		100 µg	290.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Mouse
CLONE:	MF333F
ISOTYPE:	Rat IgG2a
CONCENTRATION:	1mg/ml
FORMULATION:	Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:	Recombinant mouse FOXP3 (forkhead box protein P3).
SPECIFICITY:	Recognizes both natural and recombinant mouse FOXP3. Detects a band of ~46-49kDa by Western blot.
APPLICATION:	ELISA (direct and indirect: 1:2’000-1:10’000) Immunocytochemistry Immunohistochemistry (frozen sections) (see [3], [4]) Western Blot (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

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Product Specific Literature References

[1] Immunosurveillance of Erbb2 carcinogenesis in transgenic mice is concealed by a dominant regulatory T-cell self-tolerance: E. Ambrosino, et al.; Cancer Res. 66, 7734 (2006) Abstract
[2] Regulated compartmentalization of programmed cell death-1 discriminates CD4+CD25+ resting regulatory T cells from activated T cells: G. Raimondi, et al.; J. Immunol. 176, 2808 (2006) Abstract
[3] Dendritic cells expressing transgenic galectin-1 delay onset of autoimmune diabetes in mice: M.J. Perone, et al.; J. Immunol. 177, 5278 (2006) Abstract
[4] "Alternatively activated" dendritic cells preferentially secrete IL-10, expand Foxp3+CD4+ T cells, and induce long-term organ allograft survival in combination with CTLA4-Ig: Y.Y. Lan, et al.; J. Immunol. 177, 5868 (2006) Abstract

General Information

MANUFACTURER

Manufactured by AdipoGen, Inc.

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