ALEXIS Biochemicals: Monoclonal Antibody to Fas (human) (2R2)

ALX-805-038

Revised 23-Jul-08

Monoclonal Antibody to Fas (human) (2R2)
SYNONYMS	anti-CD95 (human) MAb (2R2) anti-APO-1 (human) MAb (2R2) anti-TNFRSF 6 MAb (human) (2R2)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Fas Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-805-038-C100		100 µg	340.00 USD
ALX-805-038B-C100	Biotin	100 µg	390.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	2R2
ISOTYPE:	Mouse IgG3
SOURCE/HOST:	Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE).
PURITY DETAIL:	Protein A-affinity purified.
FORMULATION:	2R2: Liquid. In PBS containing 1% BSA. Contains no preservatives. 2R2-Biotin: Liquid. In PBS.
IMMUNOGEN:	Mouse L cells stably overexpressing human Fas (CD95; APO-1).
SPECIFICITY:	Recognizes human Fas.
APPLICATION:	2R2: Flow Cytometry Functional Application (induces apoptosis - no cross-linking required). - Can be used in DISC (death-inducing signalling complex assays): Co-immunoprecipitates FADD and caspase-8 after receptor activation. 2R2-Biotin: Flow Cytometry Immunocytochemistry Immunohistochemistry (frozen sections, paraffin sections) - Recommended working dilutions are 1:200-1:1000.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
HANDLING:	For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.

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Product Specific Literature References

Differential regulation and ATP requirement for caspase-8 and caspase-3 activation during CD95- and anticancer drug-induced apoptosis: D. Ferrari, et al.; J. Exp. Med. 188, 979 (1998) Abstract; Full Text
Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction: S. Wesselborg, et al.; Blood 93, 3053 (1999) Abstract; Full Text
IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt, et al.; Eur. J. Immunol. 30, 1769 (2000) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

IHC (PS) Antigen Retrieval: we recommend using a citric acid solution together with microwave retrieval (5 x 5 minutes at high intensity).

Further Categories Containing This Product:

CD Antibodies • Monoclonal Antibodies

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