ALEXIS Biochemicals: Monoclonal Antibody to Hrs and Hrs-2 (A-5)

ALX-804-382

Revised 21-Feb-08

Monoclonal Antibody to Hrs and Hrs-2 (A-5)
SYNONYMS	anti-HGS MAb (A-5) anti-Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate MAb (A-5)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	Hepatocyte Growth Factor [HGF] & Receptors/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-382-C050		50 µg	290.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Monkey
CLONE:	A-5
ISOTYPE:	Mouse IgG2a
CONCENTRATION:	1mg/ml
FORMULATION:	Liquid. In PBS containing 10% glycerol.
IMMUNOGEN:	Recombinant full length rat Hrs-2 (hepatocyte growth factor-regulated tyrosine kinase substrate).
SPECIFICITY:	Recognizes human, mouse, rat and monkey Hrs (HGS) and Hrs-2.
APPLICATION:	Immunohistochemistry (1:100-1:1'000) Immunoprecipitation (2µg/ml) Western Blot (1:2'000-1:3'000)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 3 months when stored at +4°C.
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Isoform-specific exocytotic protein mRNA expression in hypothalamic magnocellular neurons: regulation after osmotic challenge: G. Jacobsson, et al.; Neuroendocrinology 70, 392 (1999) Abstract
The cellular and developmental expression of hrs-2 in rat: S. Tsujimoto, et al.; Eur. J. Neurosci. 11, 3047 (1999) Abstract
Hrs-2 regulates receptor-mediated endocytosis via interactions with Eps15: A.J. Bean, et al.; J. Biol. Chem. 275, 15271 (2000) Abstract; Full Text
Distinct protein domains are responsible for the interaction of Hrs-2 with SNAP-25. The role of Hrs-2 in 7 S complex formation: S. Tsujimoto & A.J. Bean; J. Biol. Chem. 275, 2938 (2000) Abstract; Full Text
SNAP-25-associated Hrs-2 protein colocalizes with AQP2 in rat kidney collecting duct principal cells: A. Shukla, et al.; Am. J. Physiol. Renal. Physiol. 281, F546 (2001) Abstract; Full Text
CART: an Hrs/actinin-4/BERP/myosin V protein complex required for efficient receptor recycling: Q. Yan, et al.; Mol. Biol. Cell 16, 2470 (2005) Abstract; Full Text
Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) interacts with PELP1 and activates MAPK: S.K. Rayala, et al.; J. Biol. Chem. 281, 4395 (2006) Abstract; Full Text

General Information

Hrs/Hgs (hepatocyte growth factor-regulated tyrosine kinase substrate) is a tyrosine-phosphorylated member of a protein family characterized by a conserved 3´-phosphoinositide-interacting RING zinc finger domain, the so called FYVE finger. A variant of Hrs, Hrs-2 is an ATPase that has been shown to play a regulatory role in the docking and/or fusion of synaptic vesicles to plasma membranes through a calcium-regulated interaction with SNAP-25. Hrs-2 physically interacts with Eps15, a protein required for receptor-mediated endocytosis. The Hrs-2/Eps15 interaction is calcium dependent, inhibited by SNAP-25 and alpha-adaptin, and results in the inhibition of receptor-mediated endocytosis. Immunoelectron microscopy reveals Hrs-2 localization on the limiting membrane of multivesicular bodies, organelles in the endosomal pathway. Recent data suggest that Hrs-2 acts to provide communication between endo- and exocytic processes.

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