ALEXIS Biochemicals: Monoclonal Antibody to Jagged-1 (human) (J1G53-3)

ALX-804-734

Revised 09-Jul-08

Monoclonal Antibody to Jagged-1 (human) (J1G53-3)
SYNONYMS	anti-HJ1 (human) MAb (J1G53-3) anti-CD339 (human) MAb (J1G53-3)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Delta-like [DLL] & Jagged / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-734-C100		100 µg	320.00 USD
ALX-804-734PC-C050	PerCP	50 µg	350.00 USD
ALX-804-734F-C050	FITC	50 µg	350.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	J1G53-3
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml
FORMULATION:	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:	Recombinant human Jagged-1.
SPECIFICITY:	Recognizes human Jagged-1.
APPLICATION:	ELISA (direct and indirect: 1:500-1:5’000) Flow Cytometry (1:20-1:100) Western Blot (1:500-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 1-2 weeks when stored at +4°C.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Product Images

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Product Description

Detects human full length Jagged-1 by Western blot and endogenous Jagged-1 expressed on CD4⁺ T cells by flow cytometry.

General Information

The Notch family of proteins are involved in the regulation of key biological processes such as cell fate determination, proliferation, differentiation, and apoptosis. Jagged-1 (HJ1; CD339), a cell surface glycoprotein, is one of several ligands that activate multiple Notch receptors and is involved in mediating Notch signalling. Jagged-1 signalling through Notch has been shown to play a role in hematopoiesis. Jagged-1 also seems to be involved in early and late stages of mammalian cardiovascular development. It has been shown to enhance fibroblast growth factor-induced angiogenesis (in vitro). Mutations in the human Jagged-1 gene cause Alagille syndrome.

MANUFACTURER

Manufactured by AdipoGen, Inc.

General Literature References

An antisense oligonucleotide to the notch ligand jagged enhances fibroblast growth factor-induced angiogenesis in vitro: A.B. Zimrin, et al.; J. Biol. Chem. 271, 32499 (1996) Abstract; Full Text
Mutations in the human Jagged1 gene are responsible for Alagille syndrome: T. Oda, et al.; Nat. Genet. 16, 235 (1997) Abstract
Expression of Notch-1 and its ligands, Delta-like-1 and Jagged-1, is critical for glioma cell survival and proliferation: B.W. Purow, et al.; Cancer Res. 65, 2353 (2005) Abstract; Full Text
Jagged1 protein enhances the differentiation of mesenchymal stem cells into cardiomyocytes: H. Li, et al.; BBRC 341, 320 (2006) Abstract
Endothelial expression of the Notch ligand Jagged1 is required for vascular smooth muscle development: F.A. High, et al.; PNAS 105, 1955 (2008) Abstract

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